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. 1987 Jan;55(1):211–216. doi: 10.1128/iai.55.1.211-216.1987

A murine monoclonal antibody specific for the outer core oligosaccharide of Salmonella lipopolysaccharide.

R S Tsang, K H Chan, P Y Chau, K C Wan, M H Ng, S Schlecht
PMCID: PMC260304  PMID: 2432013

Abstract

Immunoglobulin G3 murine monoclonal antibody T6 specific for the lipopolysaccharide of Salmonella O serogroups A to E was established. By using R mutants of Salmonella spp., Escherichia coli, and Shigella spp., the major reactive epitope with T6 was tentatively identified as the terminal disaccharide, N-acetylglucosamine 1.2----alpha glucose, of the core oligosaccharide. T6 was reactive with 10 clinical isolates of each of the Salmonella O serogroups A to E but not with 58 isolates of other gram-negative bacteria. Its selective reactivity against Salmonella spp. renders T6 a potentially more useful reagent than the conventional polyvalent serum for the identification of Salmonella spp. It may also serve as a useful molecular tool for the study of the outer core structure of all Salmonella and related species.

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Selected References

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