Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1987 Sep;55(9):2204–2207. doi: 10.1128/iai.55.9.2204-2207.1987

Serological response to the P fimbriae of uropathogenic Escherichia coli in pyelonephritis.

J M de Ree, J F van den Bosch
PMCID: PMC260679  PMID: 2887515

Abstract

Uropathogenic Escherichia coli strains isolated from four patients with pyelonephritis were characterized by their O:K serotype, hemolysin production, mannose-resistant hemagglutination, and the serotype of the P fimbriae. These P fimbriae were serotyped with specific monoclonal antibodies. Serum samples from the patients were analyzed for the presence of specific antibodies to the P fimbriae. In all cases antifimbrial antibodies were found, strongly suggesting that these P fimbriae are expressed in vivo. However, the antibodies in the patient sera were not able to inhibit the mannose-resistant hemagglutination. This finding suggests that these antibodies react with the fimbrial components and not with the minor components which are responsible for adhesion.

Full text

PDF
2205

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Edén C. S., Hagberg L., Hanson L. A., Korhonen T., Leffler H., Olling S. Adhesion of Escherichia coli in urinary tract infection. Ciba Found Symp. 1981;80:161–187. doi: 10.1002/9780470720639.ch11. [DOI] [PubMed] [Google Scholar]
  2. Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hagberg L., Jodal U., Korhonen T. K., Lidin-Janson G., Lindberg U., Svanborg Edén C. Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981 Feb;31(2):564–570. doi: 10.1128/iai.31.2.564-570.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Harber M. J. Bacterial adherence. Eur J Clin Microbiol. 1985 Jun;4(3):257–261. doi: 10.1007/BF02013648. [DOI] [PubMed] [Google Scholar]
  5. Harber M. J., Chick S., Mackenzie R., Asscher A. W. Lack of adherence to epithelial cells by freshly isolated urinary pathogens. Lancet. 1982 Mar 13;1(8272):586–588. doi: 10.1016/s0140-6736(82)91749-4. [DOI] [PubMed] [Google Scholar]
  6. Hull R. A., Gill R. E., Hsu P., Minshew B. H., Falkow S. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981 Sep;33(3):933–938. doi: 10.1128/iai.33.3.933-938.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Korhonen T. K., Nurmiaho E. L., Ranta H., Edén C. S. New Method for isolation of immunologically pure pili from Escherichia coli. Infect Immun. 1980 Feb;27(2):569–575. doi: 10.1128/iai.27.2.569-575.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Korhonen T. K., Väisänen-Rhen V., Rhen M., Pere A., Parkkinen J., Finne J. Escherichia coli fimbriae recognizing sialyl galactosides. J Bacteriol. 1984 Aug;159(2):762–766. doi: 10.1128/jb.159.2.762-766.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Källenius G., Möllby R., Svenson S. B., Helin I., Hultberg H., Cedergren B., Winberg J. Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet. 1981 Dec 19;2(8260-61):1369–1372. doi: 10.1016/s0140-6736(81)92797-5. [DOI] [PubMed] [Google Scholar]
  10. Orskov I., Orskov F. Serology of Escherichia coli fimbriae. Prog Allergy. 1983;33:80–105. [PubMed] [Google Scholar]
  11. Rene P., Dinolfo M., Silverblatt F. J. Serum and urogenital antibody responses to Escherichia coli pili in cystitis. Infect Immun. 1982 Nov;38(2):542–547. doi: 10.1128/iai.38.2.542-547.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rene P., Silverblatt F. J. Serological response to Escherichia coli pili in pyelonephritis. Infect Immun. 1982 Aug;37(2):749–754. doi: 10.1128/iai.37.2.749-754.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Svanborg Edén C., Bjursten L. M., Hull R., Hull S., Magnusson K. E., Moldovano Z., Leffler H. Influence of adhesins on the interaction of Escherichia coli with human phagocytes. Infect Immun. 1984 Jun;44(3):672–680. doi: 10.1128/iai.44.3.672-680.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Svanborg Edén C., Hagberg L., Hanson L. A., Hull S., Hull R., Jodal U., Leffler H., Lomberg H., Straube E. Bacterial adherence--a pathogenetic mechanism in urinary tract infections caused by Escherichia coli. Prog Allergy. 1983;33:175–188. [PubMed] [Google Scholar]
  15. Svenson S. B., Källenius G., Möllby R., Hultberg H., Winberg J. Rapid identification of P-fimbriated Escherichia coli by a receptor-specific particle agglutination test. Infection. 1982;10(4):209–214. doi: 10.1007/BF01666912. [DOI] [PubMed] [Google Scholar]
  16. Uhlin B. E., Norgren M., Båga M., Normark S. Adhesion to human cells by Escherichia coli lacking the major subunit of a digalactoside-specific pilus-adhesin. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1800–1804. doi: 10.1073/pnas.82.6.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg-Edén C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet. 1981 Dec 19;2(8260-61):1366–1369. doi: 10.1016/s0140-6736(81)92796-3. [DOI] [PubMed] [Google Scholar]
  18. Väisänen V., Korhonen T. K., Jokinen M., Gahmberg C. G., Ehnholm C. Blood group M specific haemagglutinin in pyelonephritogenic Escherichia coli. Lancet. 1982 May 22;1(8282):1192–1192. doi: 10.1016/s0140-6736(82)92264-4. [DOI] [PubMed] [Google Scholar]
  19. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies for serotyping the P fimbriae of uropathogenic Escherichia coli. J Clin Microbiol. 1986 Jul;24(1):121–125. doi: 10.1128/jcm.24.1.121-125.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies raised against Pap fimbriae recognize minor component(s) involved in receptor binding. Microb Pathog. 1987 Feb;2(2):113–121. doi: 10.1016/0882-4010(87)90103-3. [DOI] [PubMed] [Google Scholar]
  21. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies that recognize the P fimbriae F71, F72, F9, and F11 from uropathogenic Escherichia coli. Infect Immun. 1985 Dec;50(3):900–904. doi: 10.1128/iai.50.3.900-904.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Die I., van Megen I., Hoekstra W., Bergmans H. Molecular organisation of the genes involved in the production of F7(2) fimbriae, causing mannose-resistant haemagglutination, of a uropathogenic Escherichia coli 06:K2:H1:F7 strain. Mol Gen Genet. 1984;194(3):528–533. doi: 10.1007/BF00425570. [DOI] [PubMed] [Google Scholar]
  23. van den Bosch J. F., Postma P., Koopman P. A., de Graaff J., MacLaren D. M., van Brenk D. G., Guinée P. A. Virulence of urinary and faecal Escherichia coli in relation to serotype, haemolysis and haemagglutination. J Hyg (Lond) 1982 Jun;88(3):567–577. doi: 10.1017/s002217240007042x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. van der Bosch J. F., Verboom-Sohmer U., Postma P., de Graaff J., MacLaren D. M. Mannose-sensitive and mannose-resistant adherence to human uroepithelial cells and urinary virulence of Escherichia coli. Infect Immun. 1980 Jul;29(1):226–233. doi: 10.1128/iai.29.1.226-233.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES