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. 1987 Oct;55(10):2327–2331. doi: 10.1128/iai.55.10.2327-2331.1987

Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera.

W A Petri Jr 1, M P Joyce 1, J Broman 1, R D Smith 1, C F Murphy 1, J I Ravdin 1
PMCID: PMC260708  PMID: 2888730

Abstract

Cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. We determined whether human immune sera contain blocking antibody for the 170-kilodalton (kDa) galactose or N-acetylgalactosamine (Gal/GalNAc)-binding lectin of Entamoeba histolytica. By Western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here prominently recognized a 170-kDa amebic protein. Western blot of the purified Gal/GalNAc lectin with pooled human immune sera (PHIS) confirmed that the 170-kDa band was the adherence lectin. Immunoprecipitation of [35S]methionine-metabolically-labeled amebae with the antilectin monoclonal antibody H8-5 and with PHIS demonstrated that the 170-kDa lectin was the major antigen recognized by PHIS. The in vitro adherence of E. histolytica trophozoites to CHO cells at 4 degrees C was inhibited by prior exposure of amebae to greater than or equal to 1.0% PHIS. The humoral response to the Gal/GalNAc-binding lectin of the parasite may contribute to the development of protective immunity against invasive amebiasis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bracha R., Mirelman D. Adherence and ingestion of Escherichia coli serotype 055 by trophozoites of Entamoeba histolytica. Infect Immun. 1983 Jun;40(3):882–887. doi: 10.1128/iai.40.3.882-887.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calderón J., Avila E. E. Antibody-induced caps in Entamoeba histolytica: isolation and electrophoretic analysis. J Infect Dis. 1986 May;153(5):927–932. doi: 10.1093/infdis/153.5.927. [DOI] [PubMed] [Google Scholar]
  3. Chadee K., Meerovitch E. The pathogenesis of experimentally induced amebic liver abscess in the gerbil (Meriones unguiculatus). Am J Pathol. 1984 Oct;117(1):71–80. [PMC free article] [PubMed] [Google Scholar]
  4. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  5. Kettis A. A., Thorstensson R., Utter G. Antigenicity of Entamoeba histolytica strain nih 200: a survey of clinically relevant antigenic components. Am J Trop Med Hyg. 1983 May;32(3):512–522. doi: 10.4269/ajtmh.1983.32.512. [DOI] [PubMed] [Google Scholar]
  6. Krupp I. M. Antibody response in intestinal and extraintestinal amebiasis. Am J Trop Med Hyg. 1970 Jan;19(1):57–62. doi: 10.4269/ajtmh.1970.19.57. [DOI] [PubMed] [Google Scholar]
  7. Mathews H. M., Moss D. M., Visvesvara G. S. Analysis of antigens from membrane and soluble fractions of Entamoeba histolytica. J Protozool. 1986 Aug;33(3):328–334. doi: 10.1111/j.1550-7408.1986.tb05619.x. [DOI] [PubMed] [Google Scholar]
  8. Mattern C. F., Keister D. B., Caspar P. A. Experimental amebiasis. III. A rapid in vitro assay for virulence of Entamoeba histolytica. Am J Trop Med Hyg. 1978 Sep;27(5):882–887. [PubMed] [Google Scholar]
  9. Meza I., Sabanero M., Cazares F., Bryan J. Isolation and characterization of actin from Entamoeba histolytica. J Biol Chem. 1983 Mar 25;258(6):3936–3941. [PubMed] [Google Scholar]
  10. Ravdin J. I., Croft B. Y., Guerrant R. L. Cytopathogenic mechanisms of Entamoeba histolytica. J Exp Med. 1980 Aug 1;152(2):377–390. doi: 10.1084/jem.152.2.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ravdin J. I., Guerrant R. L. Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. Study with mammalian tissue culture cells and human erythrocytes. J Clin Invest. 1981 Nov;68(5):1305–1313. doi: 10.1172/JCI110377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ravdin J. I., John J. E., Johnston L. I., Innes D. J., Guerrant R. L. Adherence of Entamoeba histolytica trophozoites to rat and human colonic mucosa. Infect Immun. 1985 May;48(2):292–297. doi: 10.1128/iai.48.2.292-297.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ravdin J. I., Murphy C. F., Salata R. A., Guerrant R. L., Hewlett E. L. N-Acetyl-D-galactosamine-inhibitable adherence lectin of Entamoeba histolytica. I. Partial purification and relation to amoebic virulence in vitro. J Infect Dis. 1985 May;151(5):804–815. doi: 10.1093/infdis/151.5.804. [DOI] [PubMed] [Google Scholar]
  14. Ravdin J. I., Petri W. A., Murphy C. F., Smith R. D. Production of mouse monoclonal antibodies which inhibit in vitro adherence of Entamoeba histolytica trophozoites. Infect Immun. 1986 Jul;53(1):1–5. doi: 10.1128/iai.53.1.1-5.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salata R. A., Martinez-Palomo A., Murray H. W., Conales L., Trevino N., Segovia E., Murphy C. F., Ravdin J. I. Patients treated for amebic liver abscess develop cell-mediated immune responses effective in vitro against Entamoeba histolytica. J Immunol. 1986 Apr 1;136(7):2633–2639. [PubMed] [Google Scholar]
  16. Salata R. A., Murray H. W., Rubin B. Y., Ravdin J. I. The role of gamma interferon in the generation of human macrophages cytotoxic for Entamoeba histolytica trophozoites. Am J Trop Med Hyg. 1987 Jul;37(1):72–78. doi: 10.4269/ajtmh.1987.37.72. [DOI] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tsutsumi V., Mena-Lopez R., Anaya-Velazquez F., Martinez-Palomo A. Cellular bases of experimental amebic liver abscess formation. Am J Pathol. 1984 Oct;117(1):81–91. [PMC free article] [PubMed] [Google Scholar]
  19. Walsh J. A. Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Rev Infect Dis. 1986 Mar-Apr;8(2):228–238. doi: 10.1093/clinids/8.2.228. [DOI] [PubMed] [Google Scholar]

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