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. 1989 Oct;57(10):3221–3225. doi: 10.1128/iai.57.10.3221-3225.1989

Evidence for direct regulation of diphtheria toxin gene transcription by an Fe2+-dependent DNA-binding repressor, DtoxR, in Corynebacterium diphtheriae.

G Fourel 1, A Phalipon 1, M Kaczorek 1
PMCID: PMC260793  PMID: 2506133

Abstract

Previous studies provided indirect evidence that in Corynebacterium diphtheriae regulation of diphtheria toxin gene (tox) transcription by iron is mediated by a bacterial repressor. By performing in vitro protein-DNA binding experiments, we establish that a corynebacterial Fe2+-sensitive protein, named DtoxR, can bind to a palindromic motif present in the tox promoter region. Binding of this factor prevents the interaction of the transcription initiation machinery with presumptive critical promoter elements, providing evidence that DtoxR is responsible for the repression of toxinogenesis observed in iron-containing growth medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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