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. 1989 Oct;57(10):3226–3229. doi: 10.1128/iai.57.10.3226-3229.1989

Immunological responsiveness of chinchillas to outer membrane and isolated fimbrial proteins of nontypeable Haemophilus influenzae.

L O Bakaletz 1, B M Tallan 1, W J Andrzejewski 1, T F DeMaria 1, D J Lim 1
PMCID: PMC260794  PMID: 2570753

Abstract

Thin, nonhemagglutinating fimbriae have been demonstrated on 100% of the clinical isolates of nontypeable Haemophilus influenzae recovered from children with chronic otitis media tested in this laboratory (L. O. Bakaletz, B.M. Tallan, T.M. Hoepf, T.F. DeMaria, H.G. Birck, and D.J. Lim, Infect. Immun. 56:331-335, 1988). Chinchillas with induced otitis media responded to this surface-located antigen of both the infecting and a heterologous strain. Antibodies were found in both serum and middle ear fluids.

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Selected References

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  1. Apicella M. A., Shero M., Dudas K. C., Stack R. R., Klohs W., LaScolea L. J., Murphy T. F., Mylotte J. M. Fimbriation of Haemophilus species isolated from the respiratory tract of adults. J Infect Dis. 1984 Jul;150(1):40–43. doi: 10.1093/infdis/150.1.40. [DOI] [PubMed] [Google Scholar]
  2. Bakaletz L. O., DeMaria T. F., Lim D. J. Phagocytosis and killing of bacteria by middle ear macrophages. Arch Otolaryngol Head Neck Surg. 1987 Feb;113(2):138–144. doi: 10.1001/archotol.1987.01860020030007. [DOI] [PubMed] [Google Scholar]
  3. Bakaletz L. O., Tallan B. M., Hoepf T., DeMaria T. F., Birck H. G., Lim D. J. Frequency of fimbriation of nontypable Haemophilus influenzae and its ability to adhere to chinchilla and human respiratory epithelium. Infect Immun. 1988 Feb;56(2):331–335. doi: 10.1128/iai.56.2.331-335.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barenkamp S. J., Munson R. S., Jr, Granoff D. M. Outer membrane protein and biotype analysis of pathogenic nontypable Haemophilus influenzae. Infect Immun. 1982 May;36(2):535–540. doi: 10.1128/iai.36.2.535-540.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carlone G. M., Thomas M. L., Rumschlag H. S., Sottnek F. O. Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species. J Clin Microbiol. 1986 Sep;24(3):330–332. doi: 10.1128/jcm.24.3.330-332.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giebink G. S., Payne E. E., Mills E. L., Juhn S. K., Quie P. G. Experimental otitis media due to Streptococcus pneumoniae: immunopathogenic response in the chinchilla. J Infect Dis. 1976 Dec;134(6):595–604. doi: 10.1093/infdis/134.6.595. [DOI] [PubMed] [Google Scholar]
  7. Gnehm H. E., Pelton S. I., Gulati S., Rice P. A. Characterization of antigens from nontypable Haemophilus influenzae recognized by human bactericidal antibodies. Role of Haemophilus outer membrane proteins. J Clin Invest. 1985 May;75(5):1645–1658. doi: 10.1172/JCI111872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howie V. M., Ploussard J. H., Lester R. L., Jr Otitis media: a clinical and bacteriological correlation. Pediatrics. 1970 Jan;45(1):29–35. [PubMed] [Google Scholar]
  9. LiPuma J. J., Gilsdorf J. R. Structural and serological relatedness of Haemophilus influenzae type b pili. Infect Immun. 1988 May;56(5):1051–1056. doi: 10.1128/iai.56.5.1051-1056.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Loeb M. R., Smith D. H. Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infect Immun. 1980 Dec;30(3):709–717. doi: 10.1128/iai.30.3.709-717.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy T. F., Apicella M. A. Antigenic heterogeneity of outer membrane proteins of nontypable Haemophilus influenzae is a basis for a serotyping system. Infect Immun. 1985 Oct;50(1):15–21. doi: 10.1128/iai.50.1.15-21.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy T. F., Apicella M. A. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev Infect Dis. 1987 Jan-Feb;9(1):1–15. doi: 10.1093/clinids/9.1.1. [DOI] [PubMed] [Google Scholar]
  13. Murphy T. F., Bartos L. C., Campagnari A. A., Nelson M. B., Apicella M. A. Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae. Infect Immun. 1986 Dec;54(3):774–779. doi: 10.1128/iai.54.3.774-779.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy T. F., Bartos L. C. Human bactericidal antibody response to outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect Immun. 1988 Oct;56(10):2673–2679. doi: 10.1128/iai.56.10.2673-2679.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Murphy T. F., Bartos L. C. Purification and analysis with monoclonal antibodies of P2, the major outer membrane protein of nontypable Haemophilus influenzae. Infect Immun. 1988 May;56(5):1084–1089. doi: 10.1128/iai.56.5.1084-1089.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murphy T. F., Bartos L. C., Rice P. A., Nelson M. B., Dudas K. C., Apicella M. A. Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody. J Clin Invest. 1986 Oct;78(4):1020–1027. doi: 10.1172/JCI112656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Murphy T. F., Bernstein J. M., Dryja D. M., Campagnari A. A., Apicella M. A. Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations. J Infect Dis. 1987 Nov;156(5):723–731. doi: 10.1093/infdis/156.5.723. [DOI] [PubMed] [Google Scholar]
  18. Paterson A. J., Macsween K. F., Pennington T. H. Haemophilus influenzae subtyping by SDS-PAGE of whole-cell polypeptides. Epidemiol Infect. 1987 Aug;99(1):179–189. doi: 10.1017/s0950268800067017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Spinola S. M., Peacock J., Denny F. W., Smith D. L., Cannon J. G. Epidemiology of colonization by nontypable Haemophilus influenzae in children: a longitudinal study. J Infect Dis. 1986 Jul;154(1):100–109. doi: 10.1093/infdis/154.1.100. [DOI] [PubMed] [Google Scholar]
  20. Watanabe N., Briggs B. R., Lim D. J. Experimental otitis media in chinchillas. I. Baseline immunological investigation. Ann Otol Rhinol Laryngol Suppl. 1982 May-Jun;93:1–8. [PubMed] [Google Scholar]

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