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. 1997 Apr;89(4):259–267.

Hepatic cells' mitotic and peritoneal macrophage phagocytic activities during Trypanosoma musculi infection in zinc-deficient mice.

P A Humphrey 1, M Ashraf 1, C M Lee 1
PMCID: PMC2608211  PMID: 9145631

Abstract

The effects of zinc deficiency on hepatic cell mitotic and peritoneal macrophage phagocytic activities were examined in mice infected with Trypanosoma musculi or immunized with parasitic products. On a full-complement or pair-fed diet, infected and homogenate-inoculated mice showed mitotic activity gains of 7.9% to 80.3% and 6.5% to 99.0%, respectively. Infected and homogenate-inoculated mice on a zinc-deficient diet showed 21.8% to 95.7% and 17.2% to 65.2%, respectively, more dividing liver cells compared with controls. In comparison to controls, macrophages isolated from infected and homogenate-immunized mice on full-complement or pair-fed diets had phagocytized 13.4% to 31.4% more latex particles from day 50 to 80. In the zinc-deficient group, macrophages isolated from infected mice had significant numbers of phagocytized latex particles (1.8% to 38.5%) from day 20 to day 80 compared with controls. The homogenate-immunized mice also had increased numbers (18.6 to 30.8%) of phagocytized latex particles.

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Selected References

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  1. Aboko-Cole G. F., Lee C. M. Trypanosoma lewisi, Trypanosoma rhodesiense: mitotic activity in normal and folic acid deficient rats. Z Parasitenkd. 1975 Sep 12;47(2):71–77. doi: 10.1007/BF00382630. [DOI] [PubMed] [Google Scholar]
  2. COHN Z. A., BENSON B. THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY. J Exp Med. 1965 Jan 1;121:153–170. doi: 10.1084/jem.121.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cardoni R. L., Docampo R., Casellas A. M. Metabolic requirements for the damage of Trypanosoma cruzi epimastigotes by human polymorphonuclear leukocytes. J Parasitol. 1982 Aug;68(4):547–552. [PubMed] [Google Scholar]
  4. Chvapil M. Effect of zinc on cells and biomembranes. Med Clin North Am. 1976 Jul;60(4):799–812. [PubMed] [Google Scholar]
  5. Clinton B. A., Ortiz-Ortiz L., Garcia W., Martinez T., Capin R. Trypanosoma cruzi: early immune responses in infected mice. Exp Parasitol. 1975 Jun;37(3):417–425. doi: 10.1016/0014-4894(75)90012-0. [DOI] [PubMed] [Google Scholar]
  6. Cohn Z. A. Activation of mononuclear phagocytes: fact, fancy, and future. J Immunol. 1978 Sep;121(3):813–816. [PubMed] [Google Scholar]
  7. Cousins R. J. Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiol Rev. 1985 Apr;65(2):238–309. doi: 10.1152/physrev.1985.65.2.238. [DOI] [PubMed] [Google Scholar]
  8. Fairweather-Tait S. J. Zinc in human nutrition. Nutr Res Rev. 1988 Jan;1(1):23–37. doi: 10.1079/NRR19880005. [DOI] [PubMed] [Google Scholar]
  9. Forbes R. M., Parker H. M., Erdman J. W., Jr Effects of dietary phytate, calcium and magnesium levels on zinc bioavailability to rats. J Nutr. 1984 Aug;114(8):1421–1425. doi: 10.1093/jn/114.8.1421. [DOI] [PubMed] [Google Scholar]
  10. Fraker P. J., Gershwin M. E., Good R. A., Prasad A. Interrelationships between zinc and immune function. Fed Proc. 1986 Apr;45(5):1474–1479. [PubMed] [Google Scholar]
  11. Fraker P. J. Zinc deficiency: a common immunodeficiency state. Surv Immunol Res. 1983;2(2):155–163. [PubMed] [Google Scholar]
  12. Haynes D. C., Golub M. S., Gershwin M. E., Cheung A. T., Hurley L. S., Hendrickx A. G. Long-term marginal zinc deprivation in rhesus monkeys. I. Effects on adult female breeders before conception. Am J Clin Nutr. 1987 Jun;45(6):1492–1502. doi: 10.1093/ajcn/45.6.1492. [DOI] [PubMed] [Google Scholar]
  13. Haynes D. C., Golub M. S., Gershwin M. E., Hurley L. S., Hendrickx A. G. Long-term marginal zinc deprivation in rhesus monkeys. II. Effects on maternal health and fetal growth at midgestation. Am J Clin Nutr. 1987 Jun;45(6):1503–1513. doi: 10.1093/ajcn/45.6.1503. [DOI] [PubMed] [Google Scholar]
  14. House R. V., Dean J. H. Adoptive cell transfer studies to examine the role of T lymphocytes in immunity to Trypanosoma musculi. J Parasitol. 1988 Oct;74(5):819–827. [PubMed] [Google Scholar]
  15. Hurley L. S., Swenerton H. Congenital malformations resulting from zinc deficiency in rats. Proc Soc Exp Biol Med. 1966 Dec;123(3):692–696. doi: 10.3181/00379727-123-31578. [DOI] [PubMed] [Google Scholar]
  16. Lee C. M., Aboko-Cole F., Fletcher J. Effect of malnutrition on susceptibility of mice to Trypanosoma musculi: vitamin A-deficiency. Z Parasitenkd. 1976 Mar 31;49(1):1–10. doi: 10.1007/BF00445013. [DOI] [PubMed] [Google Scholar]
  17. Lee C. M., Lincicome D. R. Trypanosoma duttoni: oxygen uptake by liver slices of normal and pantothenate-deficient mice. Z Parasitenkd. 1971 Jun 24;36(4):346–354. doi: 10.1007/BF00259641. [DOI] [PubMed] [Google Scholar]
  18. Matossian-Rogers A. Specificity of the macrophage spreading test with reference to Leishmania antigens and correlation with delayed hypersensitivity. Clin Exp Immunol. 1979 Apr;36(1):38–45. [PMC free article] [PubMed] [Google Scholar]
  19. Menard M. P., Cousins R. J. Zinc transport by brush border membrane vesicles from rat intestine. J Nutr. 1983 Jul;113(7):1434–1442. doi: 10.1093/jn/113.7.1434. [DOI] [PubMed] [Google Scholar]
  20. Murray P. K., Jennings F. W., Murray M., Urquhart G. M. The nature of immunosuppression in Trypanosoma brucei infections in mice. I. The role of the macrophage. Immunology. 1974 Nov;27(5):815–824. [PMC free article] [PubMed] [Google Scholar]
  21. NORTH R. J., MACKANESS G. B. ELECTRON MICROSCOPICAL OBSERVATIONS ON THE PERITONEAL MACROPHAGES OF NORMAL MICE AND MICE IMMUNISED WITH LISTERIA MONOCYTOGENES. II. STRUCTURE OF MACROPHAGES FROM IMMUNE MICE AND EARLY CYTOPLASMIC RESPONSE TO THE PRESENCE OF INGESTED BACTERIA. Br J Exp Pathol. 1963 Dec;44:608–611. [PMC free article] [PubMed] [Google Scholar]
  22. Nävert B., Sandström B., Cederblad A. Reduction of the phytate content of bran by leavening in bread and its effect on zinc absorption in man. Br J Nutr. 1985 Jan;53(1):47–53. doi: 10.1079/bjn19850009. [DOI] [PubMed] [Google Scholar]
  23. PRASAD A. S., HALSTED J. A., NADIMI M. Syndrome of iron deficiency anemia, hepatosplenomegaly, hypogonadism, dwarfism and geophagia. Am J Med. 1961 Oct;31:532–546. doi: 10.1016/0002-9343(61)90137-1. [DOI] [PubMed] [Google Scholar]
  24. Pattison S. E., Cousins R. J. Zinc uptake and metabolism by hepatocytes. Fed Proc. 1986 Nov;45(12):2805–2809. [PubMed] [Google Scholar]
  25. Prasad A. S. Clinical, biochemical, and pharmacological role of zinc. Annu Rev Pharmacol Toxicol. 1979;19:393–426. doi: 10.1146/annurev.pa.19.040179.002141. [DOI] [PubMed] [Google Scholar]
  26. Richards M. P., Cousins R. J. Metallothionein and its relationship to the metabolism of dietary zinc in rats. J Nutr. 1976 Nov;106(11):1591–1599. doi: 10.1093/jn/106.11.1591. [DOI] [PubMed] [Google Scholar]
  27. Richards M. P., Cousins R. J. Zinc-binding protein: relationship to short term changes in zinc metabolism. Proc Soc Exp Biol Med. 1976 Oct;153(1):52–56. doi: 10.3181/00379727-153-39479. [DOI] [PubMed] [Google Scholar]
  28. Solomons N. W. Competitive interaction of iron and zinc in the diet: consequences for human nutrition. J Nutr. 1986 Jun;116(6):927–935. doi: 10.1093/jn/116.6.927. [DOI] [PubMed] [Google Scholar]
  29. Underwood E. J. Trace metals in human and animal health. J Hum Nutr. 1981 Feb;35(1):37–48. doi: 10.3109/09637488109143484. [DOI] [PubMed] [Google Scholar]
  30. Verma P. C., Gupta R. P., Sadana J. R., Gupta R. K. Effect of experimental zinc deficiency and repletion on some immunological variables in guinea-pigs. Br J Nutr. 1988 Jan;59(1):149–154. doi: 10.1079/bjn19880018. [DOI] [PubMed] [Google Scholar]
  31. Villalta F., Kierszenbaum F. Role of polymorphonuclear cells in Chagas' disease. I. Uptake and mechanisms of destruction of intracellular (amastigote) forms of Trypanosoma cruzi by human neutrophils. J Immunol. 1983 Sep;131(3):1504–1510. [PubMed] [Google Scholar]
  32. Wechsler D. S., Kongshavn P. A. Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice. Infect Immun. 1985 Jun;48(3):787–794. doi: 10.1128/iai.48.3.787-794.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Ziegler J. L. Cryoglobulinaemia in tropical splenomegaly syndrome. Clin Exp Immunol. 1973 Sep;15(1):65–78. [PMC free article] [PubMed] [Google Scholar]

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