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. 1986 Sep;53(3):600–605. doi: 10.1128/iai.53.3.600-605.1986

Identification of antibody classes and Fc receptors responsible for phagocytosis of Trypanosoma musculi by mouse macrophages.

P Vincendeau, M Daëron, S Daulouede
PMCID: PMC260833  PMID: 3744553

Abstract

The phagocytosis of Trypanosoma musculi by macrophages in the presence of specific antibodies was investigated. In 14-day-infected mice, opsonic antibodies were detected in serum, and phagocytosis of parasites by peritoneal macrophages was observed. The mechanism of T. musculi phagocytosis was analyzed. The binding of trypanosomes to peritoneal macrophages and J774 cells was observed in the presence of serum from hyperimmune mice and from mice infected 14 or 28 days earlier, but not in the presence of control mouse serum or sera from 7-day-infected mice. Binding was partially inhibited by mouse monoclonal immunoglobulins G1 (IgG1) or IgG2a and almost completely inhibited by a mixture of both. Binding was also partially inhibited by the anti-Fc gamma 1/gamma 2b receptor monoclonal antibody 2.4G2. Binding of T. musculi was also induced by fractions of serum from 28-day-infected mice obtained by protein A-Sepharose chromatography. Only the IgG1-rich fraction eluted at pH 6.0 and the IgG2a-rich fraction eluted at pH 4.5 promoted binding which could be almost completely inhibited by monoclonal IgG1 and IgG2a. These data indicate that IgG1 and IgG2a anti-T. musculi antibodies are responsible for the phagocytosis of T. musculi by mouse macrophages and both Fc gamma 2a and Fc gamma 1/gamma 2b receptors are involved. Such a mechanism is likely to account for the elimination of parasites in T. musculi-infected mice.

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Selected References

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  1. Albright J. W., Albright J. F. The decline of immunological resistance of aging mice to Trypanosoma musculi. Mech Ageing Dev. 1982 Dec;20(4):315–330. doi: 10.1016/0047-6374(82)90099-9. [DOI] [PubMed] [Google Scholar]
  2. Brooks B. O., Reed N. D. The effect of trypan blue on the early control of Trypanosoma musculi parasitemia in mice. J Reticuloendothel Soc. 1979 Mar;25(3):325–328. [PubMed] [Google Scholar]
  3. Chang S., Dusanic D. G. In vitro phagocytosis of Trypanosoma musculi by mouse macrophage. Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1976 Dec;9(3-4):73–82. [PubMed] [Google Scholar]
  4. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  5. Ferrante A., Jenkin C. R. Evidence implicating the mononuclear phagocytic system of the rat in immunity to infections with Trypanosoma lewisi. Aust J Exp Biol Med Sci. 1978 Apr;56(2):201–209. doi: 10.1038/icb.1978.22. [DOI] [PubMed] [Google Scholar]
  6. Ferrante A., Jenkin C. R., Reade P. C. Changes in the activity of the reticulo-endothelial system of rats during an infection with T. lewisi. Aust J Exp Biol Med Sci. 1978 Feb;56(1):47–59. doi: 10.1038/icb.1978.6. [DOI] [PubMed] [Google Scholar]
  7. Ferrante A., Jenkin C. R. The role of the macrophage in immunity to Trypanosoma lewisi infections in the rat. Cell Immunol. 1979 Feb;42(2):327–335. doi: 10.1016/0008-8749(79)90198-9. [DOI] [PubMed] [Google Scholar]
  8. Fierer J., Askonas B. A. Trypanosoma brucei infection stimulates receptor-mediated phagocytosis by murine peritoneal macrophages. Infect Immun. 1982 Sep;37(3):1282–1284. doi: 10.1128/iai.37.3.1282-1284.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenblatt H. C., Diggs C. L., Aikawa M. Antibody-dependent phagocytosis of Trypanosoma rhodesiense by murine macrophages. Am J Trop Med Hyg. 1983 Jan;32(1):34–45. doi: 10.4269/ajtmh.1983.32.34. [DOI] [PubMed] [Google Scholar]
  10. Jarvinen J. A., Dalmasso A. P. Trypanosoma musculi infections in normocomplementemic, C5-deficient, and C3-depleted mice. Infect Immun. 1977 May;16(2):557–563. doi: 10.1128/iai.16.2.557-563.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krampitz H. E. Verbreitung, Wirt-Parasit-Beziehungen und Vermehrung sizilianischer Stämme von Trypanosoma (H erpetosoma) duttoni Thiroux, 1905 (Protozoa, Trypanosomatidae) Z Parasitenkd. 1969;32(4):297–315. doi: 10.1007/BF00259643. [DOI] [PubMed] [Google Scholar]
  12. Lanham S. M. Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature. 1968 Jun 29;218(5148):1273–1274. doi: 10.1038/2181273a0. [DOI] [PubMed] [Google Scholar]
  13. Macaskill J. A., Holmes P. H., Whitelaw D. D., McConnell I., Jennings F. W., Urquhart G. M. Immunological clearance of 75Se-labelled Trypanosoma brucei in mice. II. Mechanisms in immune animals. Immunology. 1980 Aug;40(4):629–635. [PMC free article] [PubMed] [Google Scholar]
  14. Murray M., Murray P. K., Jennings F. W., Fisher E. W., Urquhart G. M. The pathology of Trypanosoma brucei infection in the rat. Res Vet Sci. 1974 Jan;16(1):77–84. [PubMed] [Google Scholar]
  15. Ralph P., Prichard J., Cohn M. Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. J Immunol. 1975 Feb;114(2 Pt 2):898–905. [PubMed] [Google Scholar]
  16. Takayanagi T., Nakatake Y., Enriquez G. L. Attachment and ingestion of Trypanosoma gambiense to the rat macrophage by specific antiserum. J Parasitol. 1974 Apr;60(2):336–339. [PubMed] [Google Scholar]
  17. Takayanagi T., Nakatke Y., Enriquez G. L. Tyrpanosoma gambiense: phagocytosis in vitro. Exp Parasitol. 1974 Aug;36(1):106–113. doi: 10.1016/0014-4894(74)90116-7. [DOI] [PubMed] [Google Scholar]
  18. Torpier G., Capron A., Ouaissi M. A. Receptor for IgG(Fc) and human beta2-microglobulin on S. mansoni schistosomula. Nature. 1979 Mar 29;278(5703):447–449. doi: 10.1038/278447a0. [DOI] [PubMed] [Google Scholar]
  19. Unkeless J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580–596. doi: 10.1084/jem.150.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vargas F. D., Viens P., Kongshavn P. A. Trypanosoma musculi infection in B-cell-deficient mice. Infect Immun. 1984 Apr;44(1):162–167. doi: 10.1128/iai.44.1.162-167.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Viens P., Dubois R., Kongshavn P. A. Platelet activity in immune lysis of Trypanosoma musculi. Int J Parasitol. 1983 Dec;13(6):527–530. doi: 10.1016/s0020-7519(83)80023-x. [DOI] [PubMed] [Google Scholar]
  22. Viens P., Targett G. A., Lumsden W. H. The immunological response of CBA mice to Trypanosoma musculi: mechanisms of protective immunity. Int J Parasitol. 1975 Apr;5(2):235–239. doi: 10.1016/0020-7519(75)90112-5. [DOI] [PubMed] [Google Scholar]
  23. Vincendeau P., Caristan A., Pautrizel R. Macrophage function during Trypanosoma musculi infection in mice. Infect Immun. 1981 Nov;34(2):378–381. doi: 10.1128/iai.34.2.378-381.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wechsler D. S., Kongshavn P. A. Characterization of antibodies mediating protection and cure of Trypanosoma musculi infection in mice. Infect Immun. 1985 Jun;48(3):787–794. doi: 10.1128/iai.48.3.787-794.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wechsler D. S., Kongshavn P. A. Cure of Trypanosoma musculi infection by heat-labile activity in immune plasma. Infect Immun. 1984 Jun;44(3):756–759. doi: 10.1128/iai.44.3.756-759.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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