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. 1986 Jun;52(3):714–717. doi: 10.1128/iai.52.3.714-717.1986

Binding of Staphylococcus aureus by human serum spreading factor in an in vitro assay.

J I Fuquay, D T Loo, D W Barnes
PMCID: PMC260916  PMID: 3710583

Abstract

Human serum spreading factor, an animal cell adhesion-promoting glycoprotein, bound Staphylococcus aureus in an assay in which association of bacteria to protein-precoated microtiter wells was quantitated. Interaction of human serum spreading factor with S. aureus suggests that this protein may play a role in host defenses and in the invasiveness and pathogenicity of microbial infections.

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Selected References

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  1. Barnes D. W., Foley T. P., Jr, Shaffer M. C., Silnutzer J. E. Human serum spreading factor: relationship to somatomedin B. J Clin Endocrinol Metab. 1984 Nov;59(5):1019–1021. doi: 10.1210/jcem-59-5-1019. [DOI] [PubMed] [Google Scholar]
  2. Barnes D. W., Reing J. E., Amos B. Heparin-binding properties of human serum spreading factor. J Biol Chem. 1985 Aug 5;260(16):9117–9122. [PubMed] [Google Scholar]
  3. Barnes D. W., Silnutzer J. Isolation of human serum spreading factor. J Biol Chem. 1983 Oct 25;258(20):12548–12552. [PubMed] [Google Scholar]
  4. Barnes D., Wolfe R., Serrero G., McClure D., Sato G. Effects of a serum spreading factor on growth and morphology of cells in serum-free medium. J Supramol Struct. 1980;14(1):47–63. doi: 10.1002/jss.400140106. [DOI] [PubMed] [Google Scholar]
  5. Hayman E. G., Pierschbacher M. D., Ohgren Y., Ruoslahti E. Serum spreading factor (vitronectin) is present at the cell surface and in tissues. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4003–4007. doi: 10.1073/pnas.80.13.4003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kessler S. W. Use of protein A-bearing staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods Enzymol. 1981;73(Pt B):442–459. doi: 10.1016/0076-6879(81)73084-2. [DOI] [PubMed] [Google Scholar]
  7. Kleinman H. K., Klebe R. J., Martin G. R. Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol. 1981 Mar;88(3):473–485. doi: 10.1083/jcb.88.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knox P., Griffiths S. A cell spreading factor in human serum that is not cold-insoluble globulin. Exp Cell Res. 1979 Oct 15;123(2):421–424. doi: 10.1016/0014-4827(79)90492-0. [DOI] [PubMed] [Google Scholar]
  9. Kuusela P. Fibronectin binds to Staphylococcus aureus. Nature. 1978 Dec 14;276(5689):718–720. doi: 10.1038/276718a0. [DOI] [PubMed] [Google Scholar]
  10. Lopes J. D., dos Reis M., Brentani R. R. Presence of laminin receptors in Staphylococcus aureus. Science. 1985 Jul 19;229(4710):275–277. doi: 10.1126/science.3160113. [DOI] [PubMed] [Google Scholar]
  11. McAbee D. D., Grinnell F. Binding and phagocytosis of fibronectin-coated beads by BHK cells: receptor specificity and dynamics. J Cell Physiol. 1985 Aug;124(2):240–246. doi: 10.1002/jcp.1041240211. [DOI] [PubMed] [Google Scholar]
  12. Mosher D. F., Proctor R. A. Binding and factor XIIIa-mediated cross-linking of a 27-kilodalton fragment of fibronectin to Staphylococcus aureus. Science. 1980 Aug 22;209(4459):927–929. doi: 10.1126/science.7403857. [DOI] [PubMed] [Google Scholar]
  13. Shaffer M. C., Foley T. P., Barnes D. W. Quantitation of spreading factor in human biologic fluids. J Lab Clin Med. 1984 May;103(5):783–791. [PubMed] [Google Scholar]
  14. Silnutzer J. E., Barnes D. W. Effects of fibronectin-related peptides on cell spreading. In Vitro Cell Dev Biol. 1985 Jan;21(1):73–78. doi: 10.1007/BF02620918. [DOI] [PubMed] [Google Scholar]
  15. Stenn K. S. Epibolin: a protein of human plasma that supports epithelial cell movement. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6907–6911. doi: 10.1073/pnas.78.11.6907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Verbrugh H. A., Peterson P. K., Smith D. E., Nguyen B. Y., Hoidal J. R., Wilkinson B. J., Verhoef J., Furcht L. T. Human fibronectin binding to staphylococcal surface protein and its relative inefficiency in promoting phagocytosis by human polymorphonuclear leukocytes, monocytes, and alveolar macrophages. Infect Immun. 1981 Sep;33(3):811–819. doi: 10.1128/iai.33.3.811-819.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamada K. M. Cell surface interactions with extracellular materials. Annu Rev Biochem. 1983;52:761–799. doi: 10.1146/annurev.bi.52.070183.003553. [DOI] [PubMed] [Google Scholar]

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