Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1986 Jun;52(3):777–785. doi: 10.1128/iai.52.3.777-785.1986

Membrane glycolipid and phospholipid composition of lipopolysaccharide-responsive and -nonresponsive murine B lymphocytes.

R Chaby, M J Morelec, D Ensergueix, R Girard
PMCID: PMC260926  PMID: 3486829

Abstract

Neutral glycolipids, gangliosides, and phospholipids present on membranes of unstimulated or lipopolysaccharide (LPS)-stimulated B cells were analyzed in LPS-responsive C3H/HePAS and LPS-nonresponsive C3H/HeJ mice. In the set of neutral glycolipids, asialo GM1 reacted preferentially with galactose oxidase but was not detectable with monospecific antibodies during immunocytofluorescence analysis. Another, more polar, neutral glycolipid appeared exclusively after stimulation of responsive B cells. Among the membrane gangliosides 1 to 5 that were able to react with galactose oxidase on B cells, ganglioside 3 was not detected in the mutant strain, and its absence was counterbalanced by the presence of a larger amount of ganglioside 1. The biosynthesis of total membrane phospholipids and the balance between phosphatidylethanolamine and phosphatidylcholine were significantly different in the two mouse strains examined and were quantitatively and qualitatively modified during the mitogenic response to LPS.

Full text

PDF
785

Images in this article

779Image
on p.779
780Image
on p.780
781Image
on p.781
781Image
on p.781

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Besancon F., Ankel H. Binding of interferon to gangliosides. Nature. 1974 Dec 6;252(5483):478–480. doi: 10.1038/252478a0. [DOI] [PubMed] [Google Scholar]
  2. Chaby R., Metezeau P., Girard R. Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes. Cell Immunol. 1984 May;85(2):531–541. doi: 10.1016/0008-8749(84)90265-x. [DOI] [PubMed] [Google Scholar]
  3. Coulon-Morelec M. J., Faure M., Maréchal J. Propriétés sérologiques des glycosides lipidiques de l'acide glucuronique. Haptènes lipidiques. Ann Inst Pasteur (Paris) 1967 Jul;113(1):37–57. [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Finne J., Krusius T., Rauvala H. Occurrence of disialosyl groups in glycoproteins. Biochem Biophys Res Commun. 1977 Jan 24;74(2):405–410. doi: 10.1016/0006-291x(77)90318-7. [DOI] [PubMed] [Google Scholar]
  6. Forni L., Coutinho A. An antiserum which recognizes lipopolysaccharide-reactive B cells in the mouse. Eur J Immunol. 1978 Jan;8(1):56–62. doi: 10.1002/eji.1830080112. [DOI] [PubMed] [Google Scholar]
  7. Fredman P., Nilsson O., Tayot J. L., Svennerholm L. Separation of gangliosides on a new type of anion-exchange resin. Biochim Biophys Acta. 1980 Apr 18;618(1):42–52. doi: 10.1016/0005-2760(80)90052-1. [DOI] [PubMed] [Google Scholar]
  8. Ghidoni R., Sonnino S., Tettamanti G., Baumann N., Reuter G., Schauer R. Isolation and characterization of a trisialoganglioside from mouse brain, containing 9-O-acetyl-N-acetylneuraminic acid. J Biol Chem. 1980 Jul 25;255(14):6990–6995. [PubMed] [Google Scholar]
  9. Gruner K. R., van Eijk R. V., Mühlradt P. F. Structure elucidation of marker glycolipids of alloantigen-activated murine T lymphocytes. Biochemistry. 1981 Aug 4;20(16):4518–4522. doi: 10.1021/bi00519a002. [DOI] [PubMed] [Google Scholar]
  10. Habu S., Kasai M., Nagai Y., Tamaoki N., TAda T., Herzenberg L. A., Okumura K. The glycolipid asialo GM1 as a new differentiation antigen of fetal thymocytes. J Immunol. 1980 Nov;125(5):2284–2288. [PubMed] [Google Scholar]
  11. Hakomori S. I. Differential reactivities of fetal and adult human erythrocytes to antisera directed against glycolipids of human erythrocytes. Vox Sang. 1969 Jun;16(6):478–485. doi: 10.1111/j.1423-0410.1969.tb04775.x. [DOI] [PubMed] [Google Scholar]
  12. Haywood A. M. Characteristics of Sendai virus receptors in a model membrane. J Mol Biol. 1974 Mar 15;83(4):427–436. doi: 10.1016/0022-2836(74)90504-x. [DOI] [PubMed] [Google Scholar]
  13. Hirata F., Toyoshima S., Axelrod J., Waxdal M. J. Phospholipid methylation: a biochemical signal modulating lymphocyte mitogenesis. Proc Natl Acad Sci U S A. 1980 Feb;77(2):862–865. doi: 10.1073/pnas.77.2.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Irwin C. C., Irwin L. N. A simple rapid method for ganglioside isolation from small amounts of tissue. Anal Biochem. 1979 Apr 15;94(2):335–339. doi: 10.1016/0003-2697(79)90369-5. [DOI] [PubMed] [Google Scholar]
  15. Jakobovits A., Sharon N., Zan-Bar I. Acquisition of mitogenic responsiveness by nonresponding lymphocytes upon insertion of appropriate membrane components. J Exp Med. 1982 Oct 1;156(4):1274–1279. doi: 10.1084/jem.156.4.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kasai M., Iwamori M., Nagai Y., Okumura K., Tada T. A glycolipid on the surface of mouse natural killer cells. Eur J Immunol. 1980 Mar;10(3):175–180. doi: 10.1002/eji.1830100304. [DOI] [PubMed] [Google Scholar]
  17. Kundu S. K., Pleatman M. A., Redwine W. A., Boyd A. E., Marcus D. M. Binding of monoclonal antibody A2B5 to gangliosides. Biochem Biophys Res Commun. 1983 Nov 15;116(3):836–842. doi: 10.1016/s0006-291x(83)80218-6. [DOI] [PubMed] [Google Scholar]
  18. Ledeen R. W., Yu R. K., Eng L. F. Gangliosides of human myelin: sialosylgalactosylceramide (G7) as a major component. J Neurochem. 1973 Oct;21(4):829–839. doi: 10.1111/j.1471-4159.1973.tb07527.x. [DOI] [PubMed] [Google Scholar]
  19. Maxwell M. A., Williams J. P. The purification of lipid extracts using Sephadex LH-20. J Chromatogr. 1967 Nov;31(1):62–68. doi: 10.1016/s0021-9673(01)86025-8. [DOI] [PubMed] [Google Scholar]
  20. Mitchell R. N., Bowers W. E. Cell surface glycoproteins of rat lymphocytes. II. Protease-sensitive glycoproteins associated with mitogeni stimulation by periodate or neuraminidase plus galactose oxidase. J Immunol. 1980 Jun;124(6):2632–2640. [PubMed] [Google Scholar]
  21. Momoi T., Tokunaga T., Nagai Y. Specific interaction of peanut agglutinin with the glycolipid asialo GM1. FEBS Lett. 1982 May 3;141(1):6–10. doi: 10.1016/0014-5793(82)80003-3. [DOI] [PubMed] [Google Scholar]
  22. Nakahara K., Ohashi T., Oda T., Hirano T., Kasai M., Okumura K., Tada T. Asialo GM1 as a cell-surface marker detected in acute lymphoblastic leukemia. N Engl J Med. 1980 Mar 20;302(12):674–677. doi: 10.1056/NEJM198003203021208. [DOI] [PubMed] [Google Scholar]
  23. O'Keefe E., Cuatecasas P. Cholera toxin and membrane gangliosides: binding and adenylate cyclase activation in normal and transformed cells. J Membr Biol. 1978 Jul 21;42(1):61–79. doi: 10.1007/BF01870394. [DOI] [PubMed] [Google Scholar]
  24. Rosenfelder G., van Eijk R. V., Mühlradt P. F. Metabolic carbohydrate-labelling of glycolipids from mouse splenocytes. Mitogen-stimulated B and T cells show different labelling patterns. Eur J Biochem. 1979 Jun;97(1):229–237. doi: 10.1111/j.1432-1033.1979.tb13107.x. [DOI] [PubMed] [Google Scholar]
  25. Rothfield L., Romeo D. Role of lipids in the biosynthesis of the bacterial cell envelope. Bacteriol Rev. 1971 Mar;35(1):14–38. doi: 10.1128/br.35.1.14-38.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SVENNERHOLM L. Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957 Jun;24(3):604–611. doi: 10.1016/0006-3002(57)90254-8. [DOI] [PubMed] [Google Scholar]
  27. Saito T., Hakomori S. I. Quantitative isolation of total glycosphingolipids from animal cells. J Lipid Res. 1971 Mar;12(2):257–259. [PubMed] [Google Scholar]
  28. Schwarting G. A., Summers A. Gangliotetraosylceramide is a T cell differentiation antigen associated with natural cell-mediated cytotoxicity. J Immunol. 1980 Apr;124(4):1691–1694. [PubMed] [Google Scholar]
  29. Sela B. A., Raz A., Geiger B. Antibodies to ganglioside GM1 induce mitogenic stimulation and cap formation in rat thymocytes. Eur J Immunol. 1978 Apr;8(4):268–274. doi: 10.1002/eji.1830080410. [DOI] [PubMed] [Google Scholar]
  30. Seyfried T. N., Ando S., Yu R. K. Isolation and characterization of human liver hematoside. J Lipid Res. 1978 Jul;19(5):538–543. [PubMed] [Google Scholar]
  31. Shier W. T., Baldwin J. H., Nilsen-Hamilton M., Hamilton R. T., Thanassi N. M. Regulation of guanylate and adenylate cyclase activities by lysolecithin. Proc Natl Acad Sci U S A. 1976 May;73(5):1586–1590. doi: 10.1073/pnas.73.5.1586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Six H. R., Young W. W., Jr, Uemura K., Kinsky S. C. Effect of antibody-complement on multiple vs. single compartment liposomes. Application of a fluorometric assay for following changes in liposomal permeability. Biochemistry. 1974 Sep 10;13(19):4050–4058. doi: 10.1021/bi00716a037. [DOI] [PubMed] [Google Scholar]
  33. Spiegel S., Kassis S., Wilchek M., Fishman P. H. Direct visualization of redistribution and capping of fluorescent gangliosides on lymphocytes. J Cell Biol. 1984 Nov;99(5):1575–1581. doi: 10.1083/jcb.99.5.1575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Stein K. E., Schwarting G. A., Marcus D. M. Glycolipid markers of murine lymphocyte subpopulations. J Immunol. 1978 Feb;120(2):676–679. [PubMed] [Google Scholar]
  35. Suzuki Y., Suzuki K. Specific radioactive labeling of terminal n-acetylgalactosamine of glycosphingolipids by the galactose oxidase-sodium borohydride method. J Lipid Res. 1972 Sep;13(5):687–690. [PubMed] [Google Scholar]
  36. Tonegawa Y., Hakomori S. I. "Ganglioprotein and globoprotein": the glycoproteins reacting with anti-ganglioside and anti-globoside antibodies and the ganglioprotein change associated with transformation. Biochem Biophys Res Commun. 1977 May 9;76(1):9–17. doi: 10.1016/0006-291x(77)91661-8. [DOI] [PubMed] [Google Scholar]
  37. Vogel S. N., Madonna G. S., Wahl L. M., Rick P. D. In vitro stimulation of C3H/HeJ spleen cells and macrophages by a lipid A precursor molecule derived from Salmonella typhimurium. J Immunol. 1984 Jan;132(1):347–353. [PubMed] [Google Scholar]
  38. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Young W. W., Jr, Durdik J. M., Urdal D., Hakomori S., Henney C. S. Glycolipid expression in lymphoma cell variants: chemical quantity, immunologic reactivity, and correlations with susceptibility to NK cells. J Immunol. 1981 Jan;126(1):1–6. [PubMed] [Google Scholar]
  40. Young W. W., Jr, MacDonald E. M., Nowinski R. C., Hakomori S. I. Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (asialo GM2). J Exp Med. 1979 Oct 1;150(4):1008–1019. doi: 10.1084/jem.150.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. van den Bosch H. Phosphoglyceride metabolism. Annu Rev Biochem. 1974;43(0):243–277. doi: 10.1146/annurev.bi.43.070174.001331. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES