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. 1985 Mar;47(3):827–830. doi: 10.1128/iai.47.3.827-830.1985

Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.

M A Taylor, K S Wise, M A McIntosh
PMCID: PMC261402  PMID: 3972456

Abstract

A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.

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Selected References

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  1. Amikam D., Glaser G., Razin S. Mycoplasmas (Mollicutes) have a low number of rRNA genes. J Bacteriol. 1984 Apr;158(1):376–378. doi: 10.1128/jb.158.1.376-378.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amikam D., Razin S., Glaser G. Ribosomal RNA genes in Mycoplasma. Nucleic Acids Res. 1982 Jul 24;10(14):4215–4222. doi: 10.1093/nar/10.14.4215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandsma J., Miller G. Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6851–6855. doi: 10.1073/pnas.77.11.6851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Freundt E. A. Present status of the medical importance of mycoplasmas. Pathol Microbiol (Basel) 1974;40(3):155–187. doi: 10.1159/000162521. [DOI] [PubMed] [Google Scholar]
  6. Glaser G., Amikam D., Razin S. Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum. Nucleic Acids Res. 1984 Mar 12;12(5):2421–2426. doi: 10.1093/nar/12.5.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leary J. J., Brigati D. J., Ward D. C. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045–4049. doi: 10.1073/pnas.80.13.4045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Razin S., Gross M., Wormser M., Pollack Y., Glaser G. Detection of mycoplasmas infecting cell cultures by DNA hybridization. In Vitro. 1984 May;20(5):404–408. doi: 10.1007/BF02619586. [DOI] [PubMed] [Google Scholar]
  9. Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reich P. R., Somerson N. L., Hybner C. J., Chanock R. M., Weissman S. M. Genetic differentiation by nucleic Acid homology I. Relationships among Mycoplasma species of man. J Bacteriol. 1966 Aug;92(2):302–310. doi: 10.1128/jb.92.2.302-310.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  12. Somerson N. L., Reich P. R., Walls B. E., Chanock R. M., Weissman S. M. Genetic Differentiation by Nucleic Acid Homology II. Genotypic Variations Within Two Mycoplasma Species. J Bacteriol. 1966 Aug;92(2):311–317. doi: 10.1128/jb.92.2.311-317.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Taylor M. A., McIntosh M. A., Robbins J., Wise K. S. Cloned genomic DNA sequences from Mycoplasma hyorhinis encoding antigens expressed in Escherichia coli. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4154–4158. doi: 10.1073/pnas.80.13.4154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wieslander L. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979 Oct 1;98(2):305–309. doi: 10.1016/0003-2697(79)90145-3. [DOI] [PubMed] [Google Scholar]
  16. Wise K. S., Cassell G. H., Action R. T. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4479–4483. doi: 10.1073/pnas.75.9.4479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wise K. S., Watson R. K. Mycoplasma hyorhinis GDL surface protein antigen p120 defined by monoclonal antibody. Infect Immun. 1983 Sep;41(3):1332–1339. doi: 10.1128/iai.41.3.1332-1339.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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