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. 1985 Aug;49(2):298–304. doi: 10.1128/iai.49.2.298-304.1985

Dissociation between interleukin-1 and interleukin-2 production in proliferative response to microbial antigens: restorative effect of exogenous interleukin-2.

D Vismara, G Lombardi, E Piccolella, V Colizzi
PMCID: PMC262014  PMID: 3894232

Abstract

The relationship between the production of interleukin-1 (IL-1) and interleukin-2 (IL-2) after stimulation of human mononuclear cells within an antigenic extract from Candida albicans was analyzed in both responder and nonresponder donors. Culture supernatants from responders contained both IL-1 and IL-2 activity, whereas the supernatants from nonresponders contained only IL-1 and no appreciable IL-2. However, the addition of exogenous IL-2 to nonresponder cultures restored the normal proliferative response. Similar observations were made when cells from mice infected intravenously with high doses of Mycobacterium bovis BCG were cultured; these cells showed a marked impairment of the proliferative response to purified protein derivative. Spleen cells from BCG-induced unresponsive mice failed to produce IL-2 despite the fact that normal IL-1 activity was present in the culture. Again, the addition of exogenous IL-2 fully reversed the proliferative unresponsiveness. Thus, the presence of IL-1 does not necessarily induce production of IL-2, and the proliferative unresponsiveness is therefore due to a primary lack of IL-2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bettens F., Kristensen F., Walker C., Schwuléra U., Bonnard G. D., de Weck A. L. Lymphokine regulation of activated (G1) lymphocytes. II. Glucocorticoid and anti-Tac-induced inhibition of human T lymphocyte proliferation. J Immunol. 1984 Jan;132(1):261–265. [PubMed] [Google Scholar]
  2. Bettens F., Kristensen F., Walker C., de Weck A. L. Human lymphocyte proliferation. II. Formation of activated (G1) cells. Eur J Immunol. 1982 Nov;12(11):948–952. doi: 10.1002/eji.1830121110. [DOI] [PubMed] [Google Scholar]
  3. Colizzi V., Ferluga J., Garreau F., Malkovsky M., Asherson G. L. Suppressor cells induced by BCG release non-specific factors in vitro which inhibit DNA synthesis and interleukin-2 production. Immunology. 1984 Jan;51(1):65–71. [PMC free article] [PubMed] [Google Scholar]
  4. Colizzi V. In vivo and in vitro administration of interleukin 2-containing preparation reverses T-cell unresponsiveness in Mycobacterium bovis BCG-infected mice. Infect Immun. 1984 Jul;45(1):25–28. doi: 10.1128/iai.45.1.25-28.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Devos R., Plaetinck G., Fiers W. Induction of cytolytic cells by pure recombinant human interleukin 2. Eur J Immunol. 1984 Nov;14(11):1057–1060. doi: 10.1002/eji.1830141118. [DOI] [PubMed] [Google Scholar]
  6. Haregewoin A., Godal T., Mustafa A. S., Belehu A., Yemaneberhan T. T-cell conditioned media reverse T-cell unresponsiveness in lepromatous leprosy. Nature. 1983 May 26;303(5915):342–344. doi: 10.1038/303342a0. [DOI] [PubMed] [Google Scholar]
  7. Harel-Bellan A., Joskowicz M., Fradelizi D., Eisen H. Modification of T-cell proliferation and interleukin 2 production in mice infected with Trypanosoma cruzi. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3466–3469. doi: 10.1073/pnas.80.11.3466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoffenbach A., Lagrange P. H., Bach M. A. Deficit of interleukin 2 production associated with impaired T-cell proliferative responses in Mycobacterium lepraemurium infection. Infect Immun. 1983 Jan;39(1):109–116. doi: 10.1128/iai.39.1.109-116.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoffenbach A., Lagrange P. H., Bach M. A. Influence of dose and route of Mycobacterium lepraemurium inoculation on the production of interleukin 1 and interleukin 2 in C57BL/6 mice. Infect Immun. 1984 Jun;44(3):665–671. doi: 10.1128/iai.44.3.665-671.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lachman L. B. Human interleukin 1: purification and properties. Fed Proc. 1983 Jun;42(9):2639–2645. [PubMed] [Google Scholar]
  11. Linker-Israeli M., Bakke A. C., Kitridou R. C., Gendler S., Gillis S., Horwitz D. A. Defective production of interleukin 1 and interleukin 2 in patients with systemic lupus erythematosus (SLE). J Immunol. 1983 Jun;130(6):2651–2655. [PubMed] [Google Scholar]
  12. Lombardi G., Piccolella E., Vismara D., Colizzi V., Asherson G. L. Candida albicans polysaccharide extract (MPPS) and PPD stimulate the production of interleukin-1 and lymphocyte proliferation. Clin Exp Immunol. 1984 Dec;58(3):581–586. [PMC free article] [PubMed] [Google Scholar]
  13. Mizel S. B. Interleukin 1 and T cell activation. Immunol Rev. 1982;63:51–72. doi: 10.1111/j.1600-065x.1982.tb00411.x. [DOI] [PubMed] [Google Scholar]
  14. Piccolella E., Lombardi G., Morelli R. Generation of suppressor cells in the response of human lymphocytes to a polysaccharide from Candida albicans. J Immunol. 1981 Jun;126(6):2151–2155. [PubMed] [Google Scholar]
  15. Piccolella E., Lombardi G., Morelli R. Human lymphocyte-activating properties of a purified polysaccharide from Candida albicans: B and T cell cooperation in the mitogenic response. J Immunol. 1980 Nov;125(5):2082–2088. [PubMed] [Google Scholar]
  16. Piccolella E., Lombardi G., Morelli R. Mitogenic response of human peripheral blood lymphocytes to a purified C. albicans polysaccharide fraction: lack of helper activities is responsible for the in vitro unresponsiveness to a second antigenic challenge. J Immunol. 1981 Jun;126(6):2156–2160. [PubMed] [Google Scholar]
  17. Thoman M. L., Weigle W. O. Cell-mediated immunity in aged mice: an underlying lesion in IL 2 synthesis. J Immunol. 1982 May;128(5):2358–2361. [PubMed] [Google Scholar]
  18. Wagner H., Hardt C., Heeg K., Röllinghoff M., Pfizenmaier K. T-cell-derived helper factor allows in vivo induction of cytotoxic T cells in nu/nu mice. Nature. 1980 Mar 20;284(5753):278–278. doi: 10.1038/284278a0. [DOI] [PubMed] [Google Scholar]
  19. Watson S., Bullock W., Nelson K., Schauf V., Gelber R., Jacobson R. Interleukin 1 production by peripheral blood mononuclear cells from leprosy patients. Infect Immun. 1984 Sep;45(3):787–789. doi: 10.1128/iai.45.3.787-789.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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