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. 1985 Jul;49(1):111–115. doi: 10.1128/iai.49.1.111-115.1985

Antibodies against the light chain of tetanus toxin in human sera.

C S Lin, W H Habig, M C Hardegree
PMCID: PMC262066  PMID: 2409006

Abstract

Tetanus toxoid elicits protective antibodies against tetanus toxin in humans and animals. It has been reported that antitoxin from immunized humans contains no anti-light chain antibodies, based on immunodiffusion and quantitative precipitin analyses. We confirmed the absence of precipitating anti-light chain antibodies in tetanus immune globulin. However, the presence of antibodies against the light chain of the toxin was shown by direct binding and inhibition analyses, using enzyme-linked immunosorbent assays. Using a neutralization inhibition test, we also found that about one-fourth of the neutralizing antibodies in tetanus immune globulin are directed against the light chain. These results suggest that the light chain of tetanus toxin contains immunogenic determinants and that antibodies directed against it may have a role in the prevention of tetanus or treatment of tetanus or both.

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Selected References

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  1. Ahnert-Hilger G., Bizzini B., Goretzki K., Müller H., Völckers C., Habermann E. Monoclonal antibodies against tetanus toxin and toxoid. Med Microbiol Immunol. 1983;172(2):123–135. doi: 10.1007/BF02124513. [DOI] [PubMed] [Google Scholar]
  2. Barile M. F., Hardegree M. C., Pittman M. Immunization against neonatal tetanus in New Guinea. 3. The toxin-neutralization test and the response of guinea-pigs to the toxoids as used in the immunization schedules in New Guinea. Bull World Health Organ. 1970;43(3):453–459. [PMC free article] [PubMed] [Google Scholar]
  3. Gigliotti F., Insel R. A. Protective human hybridoma antibody to tetanus toxin. J Clin Invest. 1982 Dec;70(6):1306–1309. doi: 10.1172/JCI110730. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Helting T. B., Nau H. H. Analysis of the immune response to papain digestion products of tetanus toxin. Acta Pathol Microbiol Immunol Scand C. 1984 Feb;92(1):59–63. doi: 10.1111/j.1699-0463.1984.tb00052.x. [DOI] [PubMed] [Google Scholar]
  5. Helting T. B., Parschat S., Engelhardt H. Structure of tetanus toxin. Demonstration and separation of a specific enzyme converting intracellular tetanus toxin to the extracellular form. J Biol Chem. 1979 Nov 10;254(21):10728–10733. [PubMed] [Google Scholar]
  6. Helting T. B., Zwisler O. Structure of tetanus toxin. I. Breakdown of the toxin molecule and discrimination between polypeptide fragments. J Biol Chem. 1977 Jan 10;252(1):187–193. [PubMed] [Google Scholar]
  7. Helting T. B., Zwisler O., Wiegandt H. Structure of tetanus toxin. II. Toxin binding to ganglioside. J Biol Chem. 1977 Jan 10;252(1):194–198. [PubMed] [Google Scholar]
  8. Kenimer J. G., Habig W. H., Hardegree M. C. Monoclonal antibodies as probes of tetanus toxin structure and function. Infect Immun. 1983 Dec;42(3):942–948. doi: 10.1128/iai.42.3.942-948.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Larrick J. W., Truitt K. E., Raubitschek A. A., Senyk G., Wang J. C. Characterization of human hybridomas secreting antibody to tetanus toxoid. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6376–6380. doi: 10.1073/pnas.80.20.6376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ledley F. D., Lee G., Kohn L. D., Habig W. H., Hardegree M. C. Tetanus toxin interactions with thyroid plasma membranes. Implications for structure and function of tetanus toxin receptors and potential pathophysiological significance. J Biol Chem. 1977 Jun 25;252(12):4049–4055. [PubMed] [Google Scholar]
  11. Matsuda M., Hara T., Yoneda M. Immunochemical studies on tetanus neurotoxin [proceedings]. Jpn J Med Sci Biol. 1978 Apr;31(2):215–217. [PubMed] [Google Scholar]
  12. Matsuda M., Makinaga G., Hirai T. Studies on the antibody composition and neutralizing activity of tetanus antitoxin sera from various species of animals in relation to the antigenic substructure of the tetanus toxin molecule. Biken J. 1983 Dec;26(4):133–143. [PubMed] [Google Scholar]
  13. Matsuda M., Yoneda M. Antigenic substructure of tetanus neurotoxin. Biochem Biophys Res Commun. 1977 Jul 11;77(1):268–274. doi: 10.1016/s0006-291x(77)80192-7. [DOI] [PubMed] [Google Scholar]
  14. Matsuda M., Yoneda M. Dissociation of tetanus neurotoxin into two polypeptide fragments. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1257–1262. doi: 10.1016/0006-291x(74)90831-6. [DOI] [PubMed] [Google Scholar]
  15. Mizuguchi J., Yoshida T., Sato Y., Nagaoka F., Kondo S., Matuhasi T. Requirement of at least two distinct monoclonal antibodies for efficient neutralization of tetanus toxin in vivo. Naturwissenschaften. 1982 Dec;69(12):597–598. doi: 10.1007/BF00396359. [DOI] [PubMed] [Google Scholar]
  16. Mizuguchi J., Yoshida T., Yamaya S., Matuhasi T. 'Clear line' formation by immunodiffusion of tetanus toxoid and mixtures of distinct monoclonal antibodies. Toxicon. 1984;22(6):975–979. doi: 10.1016/0041-0101(84)90189-2. [DOI] [PubMed] [Google Scholar]
  17. Morris N. P., Consiglio E., Kohn L. D., Habig W. H., Hardegree M. C., Helting T. B. Interaction of fragments B and C of tetanus toxin with neural and thyroid membranes and with gangliosides. J Biol Chem. 1980 Jul 10;255(13):6071–6076. [PubMed] [Google Scholar]
  18. Robinson J. P., Holladay L. A., Hash J. H., Puett D. Conformational and molecular weight studies of tetanus toxin and its major peptides. J Biol Chem. 1982 Jan 10;257(1):407–411. [PubMed] [Google Scholar]
  19. Sheppard A. J., Cussell D., Hughes M. Production and characterization of monoclonal antibodies to tetanus toxin. Infect Immun. 1984 Feb;43(2):710–714. doi: 10.1128/iai.43.2.710-714.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor C. F., Britton P., van Heyningen S. Similarities in the heavy and light chains of tetanus toxin suggested by their amino acid compositions. Biochem J. 1983 Mar 1;209(3):897–899. doi: 10.1042/bj2090897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Volk W. A., Bizzini B., Snyder R. M., Bernhard E., Wagner R. R. Neutralization of tetanus toxin by distinct monoclonal antibodies binding to multiple epitopes on the toxin molecule. Infect Immun. 1984 Sep;45(3):604–609. doi: 10.1128/iai.45.3.604-609.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wellhöner N. H. Tetanus neurotoxin. Rev Physiol Biochem Pharmacol. 1982;93:1–68. doi: 10.1007/BFb0032668. [DOI] [PubMed] [Google Scholar]

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