Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1985 Oct;50(1):225–230. doi: 10.1128/iai.50.1.225-230.1985

Purification and vaccine potential of Klebsiella capsular polysaccharides.

S J Cryz Jr, E Fürer, R Germanier
PMCID: PMC262160  PMID: 4044036

Abstract

Capsular polysaccharide (CPS) from 18 Klebsiella strains of different capsular types was isolated and characterized. Purified CPSs were composed primarily of carbohydrate with trace quantities of protein, nucleic acids, and lipopolysaccharide. All CPSs were of a high molecular weight, possessing a Kd of 0.01 to 0.11 as determined by gel filtration over Sepharose CL-4B. Low levels of lipopolysaccharide present in all preparations were responsible for the highly pyrogenic nature of one-half of the CPS preparations. Treatment of capsular material with dilute NaOH in 95% ethanol markedly reduced the pyrogenicity of all preparations and had a negligible effect on their molecular weight. The immunogenicity of the various native CPSs for mice varied considerably from serotype to serotype, but all evoked an anticapsular immunoglobulin G response. Five of 18 NaOH-treated polysaccharides were significantly (P less than 0.05) less immunogenic than their native counterparts. Human immunoglobulin G prepared from volunteers immunized with either native or NaOH-treated KP1-0 capsular polysaccharide was equally effective at preventing experimental fatal Klebsiella pneumoniae burn wound sepsis in mice.

Full text

PDF
229

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryan C. S., Reynolds K. L., Brenner E. R. Analysis of 1,186 episodes of gram-negative bacteremia in non-university hospitals: the effects of antimicrobial therapy. Rev Infect Dis. 1983 Jul-Aug;5(4):629–638. doi: 10.1093/clinids/5.4.629. [DOI] [PubMed] [Google Scholar]
  2. Cooper J. M., Rowley D. Resistance to Klebsiella pneumoniae and the importance of two bacterial antigens. Aust J Exp Biol Med Sci. 1982 Dec;60(6):629–641. doi: 10.1038/icb.1982.65. [DOI] [PubMed] [Google Scholar]
  3. Cross A., Allen J. R., Burke J., Ducel G., Harris A., John J., Johnson D., Lew M., MacMillan B., Meers P. Nosocomial infections due to Pseudomonas aeruginosa: review of recent trends. Rev Infect Dis. 1983 Nov-Dec;5 (Suppl 5):S837–S845. doi: 10.1093/clinids/5.supplement_5.s837. [DOI] [PubMed] [Google Scholar]
  4. Cryz S. J., Jr, Fürer E., Germanier R. Prevention of fatal experimental burn-wound sepsis due to Klebsiella pneumoniae KP1-O by immunization with homologous capsular polysaccharide. J Infect Dis. 1984 Dec;150(6):817–822. doi: 10.1093/infdis/150.6.817. [DOI] [PubMed] [Google Scholar]
  5. Cryz S. J., Jr, Fürer E., Germanier R. Prevention of fatal experimental burn-wound sepsis due to Klebsiella pneumoniae KP1-O by immunization with homologous capsular polysaccharide. J Infect Dis. 1984 Dec;150(6):817–822. doi: 10.1093/infdis/150.6.817. [DOI] [PubMed] [Google Scholar]
  6. Cryz S. J., Jr, Fürer E., Germanier R. Protection against fatal Klebsiella pneumoniae burn wound sepsis by passive transfer of anticapsular polysaccharide. Infect Immun. 1984 Jul;45(1):139–142. doi: 10.1128/iai.45.1.139-142.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cryz S. J., Jr, Fürer E., Germanier R. Protection against fatal Pseudomonas aeruginosa burn wound sepsis by immunization with lipopolysaccharide and high-molecular-weight polysaccharide. Infect Immun. 1984 Mar;43(3):795–799. doi: 10.1128/iai.43.3.795-799.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cryz S. J., Jr, Fürer F., Germanier R. Experimental Klebsiella pneumoniae burn wound sepsis: role of capsular polysaccharide. Infect Immun. 1984 Jan;43(1):440–441. doi: 10.1128/iai.43.1.440-441.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cryz S. J. Progress in immunization against Klebsiella infections. Eur J Clin Microbiol. 1983 Dec;2(6):523–528. doi: 10.1007/BF02016559. [DOI] [PubMed] [Google Scholar]
  10. Daschner F. Krankenhausinfektionen in einem Universitätsklinikum. Prospektive Analyse von 39 802 Patienten. Dtsch Med Wochenschr. 1981 Jan 23;106(4):101–105. doi: 10.1055/s-2008-1070266. [DOI] [PubMed] [Google Scholar]
  11. Domenico P., Johanson W. G., Jr, Straus D. C. Lobar pneumonia in rats produced by clinical isolates of Klebsiella pneumoniae. Infect Immun. 1982 Jul;37(1):327–335. doi: 10.1128/iai.37.1.327-335.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gotschlich E. C., Liu T. Y., Artenstein M. S. Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides. J Exp Med. 1969 Jun 1;129(6):1349–1365. doi: 10.1084/jem.129.6.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gotschlich E. C., Rey M., Triau R., Sparks K. J. Quantitative determination of the human immune response to immunization with meningococcal vaccines. J Clin Invest. 1972 Jan;51(1):89–96. doi: 10.1172/JCI106801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Graybill J. R., Marshall L. W., Charache P., Wallace C. K., Melvin V. B. Nosocomial pneumonia. A continuing major problem. Am Rev Respir Dis. 1973 Nov;108(5):1130–1140. doi: 10.1164/arrd.1973.108.5.1130. [DOI] [PubMed] [Google Scholar]
  15. Griffiss J. M., Brandt B. L., Altieri P. L., Pier G. B., Berman S. L. Safety and immunogenicity of group Y and group W135 meningococcal capsular polysaccharide vaccines in adults. Infect Immun. 1981 Dec;34(3):725–732. doi: 10.1128/iai.34.3.725-732.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Howard J. G., Zola H., Christie G. H., Courtenay B. M. Studies on immunological paralysis. V. The influence of molecular weight on the immunogenicity, tolerogenicity and antibody-neutralizing activity of the 3 pneumococcal polysaccharide. Immunology. 1971 Sep;21(3):535–546. [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. MAIZTEGUI J. I., BIEGELEISEN J. Z., Jr, CHERRY W. B., KASS E. H. BACTEREMIA DUE TO GRAM-NEGATIVE RODS. A CLINICAL, BACTERIOLOGIC, SEROLOGIC AND IMMUNOFLUORESCENT STUDY. N Engl J Med. 1965 Feb 4;272:222–229. doi: 10.1056/NEJM196502042720502. [DOI] [PubMed] [Google Scholar]
  19. Markowitz S. M., Veazey J. M., Jr, Macrina F. L., Mayhall C. G., Lamb V. A. Sequential outbreaks of infection due to Klebsiella pneumoniae in a neonatal intensive care unit: implication of a conjugative R plasmid. J Infect Dis. 1980 Jul;142(1):106–112. doi: 10.1093/infdis/142.1.106. [DOI] [PubMed] [Google Scholar]
  20. McGowan J. E., Jr, Barnes M. W., Finland M. Bacteremia at Boston City Hospital: Occurrence and mortality during 12 selected years (1935-1972), with special reference to hospital-acquired cases. J Infect Dis. 1975 Sep;132(3):316–335. doi: 10.1093/infdis/132.3.316. [DOI] [PubMed] [Google Scholar]
  21. Montgomerie J. Z., Ota J. K. Klebsiella bacteremia. Arch Intern Med. 1980 Apr;140(4):525–527. [PubMed] [Google Scholar]
  22. Nimmich W. Zur Isolierung und qualitativen Bausteinanalyse der K-Antigene von Klebsiellen. Z Med Mikrobiol Immunol. 1968;154(2):117–131. [PubMed] [Google Scholar]
  23. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Seid R. C., Jr, Sadoff J. C. Preparation and characterization of detoxified lipopolysaccharide-protein conjugates. J Biol Chem. 1981 Jul 25;256(14):7305–7310. [PubMed] [Google Scholar]
  25. Wong K. H., Barrera O., Sutton A., May J., Hochstein D. H., Robbins J. D., Robbins J. B., Parkman P. D., Seligmann E. B., Jr Standardization and control of meningococcal vaccines, group A and group C polysaccharides. J Biol Stand. 1977;5(3):197–215. doi: 10.1016/s0092-1157(77)80005-x. [DOI] [PubMed] [Google Scholar]
  26. Young L. S. Nosocomial infections in the immunocompromised adult. Am J Med. 1981 Feb;70(2):398–404. doi: 10.1016/0002-9343(81)90779-8. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES