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. 1993 Feb;31(2):467–469. doi: 10.1128/jcm.31.2.467-469.1993

Type 5 and 8 capsular polysaccharides are expressed by Staphylococcus aureus isolates from rabbits, poultry, pigs, and horses.

B Poutrel 1, L Sutra 1
PMCID: PMC262793  PMID: 8432841

Abstract

A total of 103 Staphylococcus aureus isolates from rabbits (n = 37), poultry (n = 33), pigs (n = 27), and horses (n = 6) and 14 Staphylococcus intermedius isolates from wild animals were serotyped for capsular polysaccharide types 5 and 8 by an enzyme-linked immunosorbent assay using polyclonal rabbit antibodies. About 98% of the S. aureus isolates were typeable. Type 5 was predominant in the poultry (75.8%) and pig (66.7%) isolates, whereas type 8 was more frequent among the isolates from rabbits (59.5%) and horses (83.3%). By contrast, none of the 14 S. intermedius isolates was typeable.

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Selected References

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  1. Arbeit R. D., Karakawa W. W., Vann W. F., Robbins J. B. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis. 1984 Apr;2(2):85–91. doi: 10.1016/0732-8893(84)90002-6. [DOI] [PubMed] [Google Scholar]
  2. Devriese L. A. A simplified system for biotyping Staphylococcus aureus strains isolated from animal species. J Appl Bacteriol. 1984 Apr;56(2):215–220. doi: 10.1111/j.1365-2672.1984.tb01341.x. [DOI] [PubMed] [Google Scholar]
  3. Fournier J. M., Hannon K., Moreau M., Karakawa W. W., Vann W. F. Isolation of type 5 capsular polysaccharide from Staphylococcus aureus. Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):561–567. doi: 10.1016/0769-2609(87)90041-x. [DOI] [PubMed] [Google Scholar]
  4. Fournier J. M., Vann W. F., Karakawa W. W. Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide. Infect Immun. 1984 Jul;45(1):87–93. doi: 10.1128/iai.45.1.87-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hochkeppel H. K., Braun D. G., Vischer W., Imm A., Sutter S., Staeubli U., Guggenheim R., Kaplan E. L., Boutonnier A., Fournier J. M. Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide types 5 and 8. J Clin Microbiol. 1987 Mar;25(3):526–530. doi: 10.1128/jcm.25.3.526-530.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Karakawa W. W., Fournier J. M., Vann W. F., Arbeit R., Schneerson R. S., Robbins J. B. Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1985 Sep;22(3):445–447. doi: 10.1128/jcm.22.3.445-447.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karakawa W. W., Sutton A., Schneerson R., Karpas A., Vann W. F. Capsular antibodies induce type-specific phagocytosis of capsulated Staphylococcus aureus by human polymorphonuclear leukocytes. Infect Immun. 1988 May;56(5):1090–1095. doi: 10.1128/iai.56.5.1090-1095.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Plommet M. G., Wilson J. B. Serological typing of Staphylococcus aureus from wild animals. J Comp Pathol. 1969 Oct;79(4):425–433. doi: 10.1016/0021-9975(69)90062-0. [DOI] [PubMed] [Google Scholar]
  9. Poutrel B., Boutonnier A., Sutra L., Fournier J. M. Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk. J Clin Microbiol. 1988 Jan;26(1):38–40. doi: 10.1128/jcm.26.1.38-40.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Poutrel B., Mendolia C., Sutra L., Fournier J. M. Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8. J Clin Microbiol. 1990 Feb;28(2):358–360. doi: 10.1128/jcm.28.2.358-360.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sheagren J. N. Staphylococcus aureus. The persistent pathogen (first of two parts). N Engl J Med. 1984 May 24;310(21):1368–1373. doi: 10.1056/NEJM198405243102107. [DOI] [PubMed] [Google Scholar]
  12. Sheagren J. N. Staphylococcus aureus. The persistent pathogen (second of two parts). N Engl J Med. 1984 May 31;310(22):1437–1442. doi: 10.1056/NEJM198405313102206. [DOI] [PubMed] [Google Scholar]
  13. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sutra L., Rainard P., Poutrel B. Phagocytosis of mastitis isolates of Staphylococcus aureus and expression of type 5 capsular polysaccharide are influenced by growth in the presence of milk. J Clin Microbiol. 1990 Oct;28(10):2253–2258. doi: 10.1128/jcm.28.10.2253-2258.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yoshida K. Demonstration of Serologically Different Capsular Types Among Strains of Staphylococcus aureus by the Serum-Soft Agar Technique. Infect Immun. 1971 Apr;3(4):535–539. doi: 10.1128/iai.3.4.535-539.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshida K. Isolation of an additional capsular-type strain of Staphylococcus aureus by the serum-soft agar technique. Infect Immun. 1972 May;5(5):833–834. doi: 10.1128/iai.5.5.833-834.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yoshida K., Nakamura A., Otomo T., Iwami S. Detection of capsular antigen production in unencapsulated strains of Staphylococcus aureus. Infect Immun. 1974 Apr;9(4):620–623. doi: 10.1128/iai.9.4.620-623.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoshida K., Takahashi M., Ohtomo T., Minegishi Y., Ichiman Y., Haga K., Kono E., San Clemente C. L. Application of fluorescent antibody for detecting capsular substances in Staphylococcus aureus. J Appl Bacteriol. 1979 Feb;46(1):147–152. doi: 10.1111/j.1365-2672.1979.tb02592.x. [DOI] [PubMed] [Google Scholar]

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