Abstract
Two modifications that simplify and shorten a method for adsorption of the antibodies against the antigens expressed on both blastospore and germ tube cell wall surfaces (methods 2 and 3) were compared with the original method of adsorption (method 1) to detect anti-Candida albicans germ tube antibodies in 154 serum specimens. Adsorption of the sera by both modified methods resulted in titers very similar to those obtained by the original method. Only 5.2% of serum specimens tested by method 2 and 5.8% of serum specimens tested by method 3 presented greater than one dilution discrepancies in the titers with respect to the titer observed by method 1. When a test based on method 2 was evaluated with sera from patients with invasive candidiasis, the best discriminatory results (sensitivity, 84.6%; specificity, 87.9%; positive predictive value, 75.9%; negative predictive value, 92.7%; efficiency, 86.9%) were obtained when a titer of > or = 1:160 was considered positive.
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- Edwards J. E., Jr Invasive candida infections--evolution of a fungal pathogen. N Engl J Med. 1991 Apr 11;324(15):1060–1062. doi: 10.1056/NEJM199104113241511. [DOI] [PubMed] [Google Scholar]
- Kozinn P. J., Taschdjian C. L., Goldberg P. K., Protzmann W. P., MacKenzie D. W., Remington J. S., Anderson S., Seelig M. S. Efficiency of serologic tests in the diagnosis of systemic candidiasis. Am J Clin Pathol. 1978 Dec;70(6):893–898. doi: 10.1093/ajcp/70.6.893. [DOI] [PubMed] [Google Scholar]
- Pfaller M. A. Laboratory aids in the diagnosis of invasive candidiasis. Mycopathologia. 1992 Nov;120(2):65–72. doi: 10.1007/BF00578290. [DOI] [PubMed] [Google Scholar]
- Ponton J., Jones J. M. Analysis of cell wall extracts of Candida albicans by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot techniques. Infect Immun. 1986 Sep;53(3):565–572. doi: 10.1128/iai.53.3.565-572.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quindós G., Pontón J., Cisterna R. Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis. Eur J Clin Microbiol. 1987 Apr;6(2):142–146. doi: 10.1007/BF02018195. [DOI] [PubMed] [Google Scholar]
- Quindós G., Pontón J., Cisterna R., Mackenzie D. W. Value of detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidosis. Eur J Clin Microbiol Infect Dis. 1990 Mar;9(3):178–183. doi: 10.1007/BF01963834. [DOI] [PubMed] [Google Scholar]
- Quindós G., Rowe I. F., Higgens C. S., Pontón J., Cisterna R., Mackenzie D. W. Candidal infection of bone. Assessment of serologic tests in diagnosis and management. Diagn Microbiol Infect Dis. 1990 Jul-Aug;13(4):297–302. doi: 10.1016/0732-8893(90)90020-v. [DOI] [PubMed] [Google Scholar]
- Villalba R., González A. I., Linares M. J., Casal M., Torres A. Detection of antibodies to Candida albicans germ tube as a possible aid in diagnosing systemic candidiasis in bone marrow transplant patients. Eur J Clin Microbiol Infect Dis. 1993 May;12(5):347–349. doi: 10.1007/BF01964431. [DOI] [PubMed] [Google Scholar]