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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Mar;32(3):819–822. doi: 10.1128/jcm.32.3.819-822.1994

Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.

K Hashinaka 1, S Hashida 1, K Hirota 1, A Saitoh 1, A Nakata 1, H Shinagawa 1, S Oka 1, K Shimada 1, E Ishikawa 1
PMCID: PMC263130  PMID: 7515074

Abstract

Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.

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Selected References

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