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. 2001 Jan-Feb;7(1):66–72. doi: 10.3201/eid0701.010111

Lack of evidence of endogenous avian leukosis virus and endogenous avian retrovirus transmission to measles, mumps, and rubella vaccine recipients.

A I Hussain 1, V Shanmugam 1, W M Switzer 1, S X Tsang 1, A Fadly 1, D Thea 1, R Helfand 1, W J Bellini 1, T M Folks 1, W Heneine 1
PMCID: PMC2631681  PMID: 11266296

Abstract

The identification of endogenous avian leukosis virus (ALV) and endogenous avian retrovirus (EAV) in chick cell-derived measles and mumps vaccines in current use has raised concern about transmission of these retroviruses to vaccine recipients. We used serologic and molecular methods to analyze specimens from 206 recipients of measles, mumps, and rubella (MMR) vaccine for evidence of infection with ALV and EAV. A Western blot assay for detecting antibodies to endogenous ALV was developed and validated. All serum samples were negative for antibodies to endogenous ALV by Western blot analysis. Peripheral blood lymphocyte samples from 100 vaccinees were further tested by polymerase chain reaction for both ALV and EAV proviral sequences; all were negative. Matching serum samples were tested by reverse transcriptase polymerase chain reaction for ALV and EAV RNA, and all 100 samples were negative, providing no evidence of viremia. These findings do not indicate the presence of either ALV or EAV infection in MMR vaccine recipients and provide support for current immunization policies.

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Selected References

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  1. Adkins H. B., Brojatsch J., Naughton J., Rolls M. M., Pesola J. M., Young J. A. Identification of a cellular receptor for subgroup E avian leukosis virus. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11617–11622. doi: 10.1073/pnas.94.21.11617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyce-Jacino M. T., O'Donoghue K., Faras A. J. Multiple complex families of endogenous retroviruses are highly conserved in the genus Gallus. J Virol. 1992 Aug;66(8):4919–4929. doi: 10.1128/jvi.66.8.4919-4929.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Böni J., Stalder J., Reigel F., Schüpbach J. Detectionof reverse transcriptase activity in live attenuated virus vaccines. Clin Diagn Virol. 1996 Feb;5(1):43–53. doi: 10.1016/0928-0197(95)00159-x. [DOI] [PubMed] [Google Scholar]
  4. Coffin J. M., Tsichlis P. N., Conklin K. F., Senior A., Robinson H. L. Genomes of endogenous and exogenous avian retroviruses. Virology. 1983 Apr 15;126(1):51–72. doi: 10.1016/0042-6822(83)90461-0. [DOI] [PubMed] [Google Scholar]
  5. Dunwiddie C., Faras A. J. Presence of retrovirus reverse transcriptase-related gene sequences in avian cells lacking endogenous avian leukosis viruses. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5097–5101. doi: 10.1073/pnas.82.15.5097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fadly A. M. Avian retroviruses. Vet Clin North Am Food Anim Pract. 1997 Mar;13(1):71–85. doi: 10.1016/s0749-0720(15)30365-0. [DOI] [PubMed] [Google Scholar]
  7. Fisher S. G., Weber L., Carbone M. Cancer risk associated with simian virus 40 contaminated polio vaccine. Anticancer Res. 1999 May-Jun;19(3B):2173–2180. [PubMed] [Google Scholar]
  8. Harris R. J., Dougherty R. M., Biggs P. M., Payne L. N., Goffe A. P., Churchill A. E., Mortimer R. Contaminant viruses in two live virus vaccines produced in chick cells. J Hyg (Lond) 1966 Mar;64(1):1–7. doi: 10.1017/s0022172400040286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Helfand R. F., Gary H. E., Jr, Atkinson W. L., Nordin J. D., Keyserling H. L., Bellini W. J. Decline of measles-specific immunoglobulin M antibodies after primary measles, mumps, and rubella vaccination. Clin Diagn Lab Immunol. 1998 Mar;5(2):135–138. doi: 10.1128/cdli.5.2.135-138.1998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MARKHAM F. S., LEVINE S. THE ABSENCE OF SEROLOGIC RESPONSES BY CHILDREN AND ADULTS TO AVIAN LEUKOSIS VIRUS IN MEASLES VACCINE. Arch Gesamte Virusforsch. 1965;16:305–310. doi: 10.1007/BF01253827. [DOI] [PubMed] [Google Scholar]
  11. Maudru T., Peden K. W. Analysis of a coded panel of licensed vaccines by polymerase chain reaction-based reverse transcriptase assays: a collaborative study [seecomments]. J Clin Virol. 1998 Jul 24;11(1):19–28. doi: 10.1016/s0928-0197(98)00044-0. [DOI] [PubMed] [Google Scholar]
  12. Mulder J., McKinney N., Christopherson C., Sninsky J., Greenfield L., Kwok S. Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection. J Clin Microbiol. 1994 Feb;32(2):292–300. doi: 10.1128/jcm.32.2.292-300.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Parkman P. D. Safety of biopharmaceuticals: a current perspective. Dev Biol Stand. 1996;88:5–7. [PubMed] [Google Scholar]
  14. Paul D. A., Falk L. A., Kessler H. A., Chase R. M., Blaauw B., Chudwin D. S., Landay A. L. Correlation of serum HIV antigen and antibody with clinical status in HIV-infected patients. J Med Virol. 1987 Aug;22(4):357–363. doi: 10.1002/jmv.1890220408. [DOI] [PubMed] [Google Scholar]
  15. Resnick R. M., Boyce-Jacino M. T., Fu Q., Faras A. J. Phylogenetic distribution of the novel avian endogenous provirus family EAV-0. J Virol. 1990 Oct;64(10):4640–4653. doi: 10.1128/jvi.64.10.4640-4653.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richman A. V., Aulisio C. G., Jahnes W. G., Tauraso N. M. Avian leukosis antibody response in individuals given chicken embryo derived vaccines. Proc Soc Exp Biol Med. 1972 Jan;139(1):235–237. doi: 10.3181/00379727-139-36117. [DOI] [PubMed] [Google Scholar]
  17. Robertson J. S., Nicolson C., Riley A. M., Bentley M., Dunn G., Corcoran T., Schild G. C., Minor P. Assessing the significance of reverse transcriptase activity in chick cell-derived vaccines. Biologicals. 1997 Dec;25(4):403–414. doi: 10.1006/biol.1997.0111. [DOI] [PubMed] [Google Scholar]
  18. Thomas P. A., Weedon J., Krasinski K., Abrams E., Shaffer N., Matheson P., Bamji M., Kaul A., Hutson D., Grimm K. T. Maternal predictors of perinatal human immunodeficiency virus transmission. The New York City Perinatal HIV Transmission Collaborative Study Group. Pediatr Infect Dis J. 1994 Jun;13(6):489–495. doi: 10.1097/00006454-199406000-00005. [DOI] [PubMed] [Google Scholar]
  19. Tsang S. X., Switzer W. M., Shanmugam V., Johnson J. A., Goldsmith C., Wright A., Fadly A., Thea D., Jaffe H., Folks T. M. Evidence of avian leukosis virus subgroup E and endogenous avian virus in measles and mumps vaccines derived from chicken cells: investigation of transmission to vaccine recipients. J Virol. 1999 Jul;73(7):5843–5851. doi: 10.1128/jvi.73.7.5843-5851.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Waters T. D., Anderson P. S., Jr, Beebe G. W., Miller R. W. Yellow fever vaccination, avian leukosis virus, and cancer risk in man. Science. 1972 Jul 7;177(4043):76–77. doi: 10.1126/science.177.4043.76. [DOI] [PubMed] [Google Scholar]
  21. Weissmahr R. N., Schüpbach J., Böni J. Reverse transcriptase activity in chicken embryo fibroblast culture supernatants is associated with particles containing endogenous avian retrovirus EAV-0 RNA. J Virol. 1997 Apr;71(4):3005–3012. doi: 10.1128/jvi.71.4.3005-3012.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Welsh R. M., Jr, Cooper N. R., Jensen F. C., Oldstone M. B. Human serum lyses RNA tumour viruses. Nature. 1975 Oct 16;257(5527):612–614. doi: 10.1038/257612a0. [DOI] [PubMed] [Google Scholar]

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