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. 1984 Aug;45(2):428–432. doi: 10.1128/iai.45.2.428-432.1984

Transposon-mediated mutagenesis and recombination in Vibrio cholerae.

J W Newland, B A Green, R K Holmes
PMCID: PMC263247  PMID: 6086528

Abstract

An efficient method for introducing transposons into the Vibrio cholerae chromosome or the P plasmid was developed by using F'tslac+ plasmids from Escherichia coli as suicide delivery vehicles. Hybrid P::Tn1, Tn5 and P::Tn1, Tn10 plasmids containing Tn5 or Tn10 in either of the two possible orientations were constructed. During conjugation, these hybrid plasmids mediated oriented transfer of markers from the sites of insertion of homologous transposons in the donor chromosome. The collection of donor strains described here permits oriented mobilization of all parts of the V. cholerae chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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