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. 1984 Jan;43(1):66–71. doi: 10.1128/iai.43.1.66-71.1984

Effect of type A Pasteurella multocida fractions on bovine polymorphonuclear leukocyte functions.

H Ryu, M L Kaeberle, J A Roth, R W Griffith
PMCID: PMC263389  PMID: 6690417

Abstract

The effect of various Pasteurella multocida fractions on bovine polymorphonuclear leukocyte (PMN) functions was examined in vitro by using two encapsulated strains, P-2383 and P-1062 (both are Carter capsular type A and of bovine origin). The ability of PMNs to ingest Staphylococcus aureus and iodinate protein was significantly inhibited in the presence of live cells, heat-killed whole cells, or saline-extracted capsules but not in the presence of the decapsulated heat-killed cells. None of the fractions of the two strains inhibited nitroblue tetrazolium reduction by PMNs. The saline extract did not inhibit the binding of iodine to protein by a reaction involving xanthine, xanthine oxidase, and horseradish peroxidase. The PMN inhibitory factor was further characterized as a heat-stable capsular material of greater than 300,000 molecular weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belding M. E., Klebanoff S. J., Ray C. G. Peroxidase-mediated virucidal systems. Science. 1970 Jan 9;167(3915):195–196. doi: 10.1126/science.167.3915.195. [DOI] [PubMed] [Google Scholar]
  2. CARTER G. R., ANNAU E. Isolation of capsular polysaccharides from colonial variants of Pasteurella multocida. Am J Vet Res. 1953 Jul;14(52):475–478. [PubMed] [Google Scholar]
  3. Carter G. R. Pasteurellosis: Pasteurella multocida and Pasteurella hemolytica. Adv Vet Sci. 1967;11:321–379. [PubMed] [Google Scholar]
  4. Carter G. R., Rundell S. W. Identification of type A strains of P multocida using staphylococcal hyaluronidase. Vet Rec. 1975 Apr 12;96(15):343–343. doi: 10.1136/vr.96.15.343. [DOI] [PubMed] [Google Scholar]
  5. Collins F. M. Mechanisms of acquired resistance to Pasteurella multocida infection: a review. Cornell Vet. 1977 Jan;67(1):103–138. [PubMed] [Google Scholar]
  6. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  7. Gaunt G., Moffat R., Mukkur T. K. Fowl cholera: immunization of chickens with potassium thiocyanate (KSCN) extract of Pasteurella multocida serotype 3. Avian Dis. 1977 Oct-Dec;21(4):543–548. [PubMed] [Google Scholar]
  8. Klebanoff S. J., Clark R. A. Iodination by human polymorphonuclear leukocytes: a re-evaluation. J Lab Clin Med. 1977 Mar;89(3):675–686. [PubMed] [Google Scholar]
  9. Kodama H., Matsumoto M., Snow L. M. Immunogenicity of capsular antigens of Pasteurella multocida in turkeys. Am J Vet Res. 1981 Oct;42(10):1838–1841. [PubMed] [Google Scholar]
  10. Larson K. A., Schell K. R. Toxicity and antigenicity of shipping fever vaccines in calves. J Am Vet Med Assoc. 1969 Aug 1;155(3):495–499. [PubMed] [Google Scholar]
  11. Maheswaran S. K., Thies E. S. Influence of encapsulation on phagocytosis of Pasteurella multocida by bovine neutrophils. Infect Immun. 1979 Oct;26(1):76–81. doi: 10.1128/iai.26.1.76-81.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Markham R. J., Wilkie B. N. Interaction between Pasteurella haemolytica and bovine alveolar macrophages: cytotoxic effect on macrophages and impaired phagocytosis. Am J Vet Res. 1980 Jan;41(1):18–22. [PubMed] [Google Scholar]
  13. Mukkur T. K. Immunogenicity of a chaotropically extracted protective antigen(s) of Pasteurella multocida type A (bovine origin) against experimental pasteurellosis in mice. J Gen Microbiol. 1979 Jul;113(1):37–43. doi: 10.1099/00221287-113-1-37. [DOI] [PubMed] [Google Scholar]
  14. Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem. 1981 Sep 1;116(1):53–64. doi: 10.1016/0003-2697(81)90321-3. [DOI] [PubMed] [Google Scholar]
  15. Root R. K., Cohen M. S. The microbicidal mechanisms of human neutrophils and eosinophils. Rev Infect Dis. 1981 May-Jun;3(3):565–598. doi: 10.1093/clinids/3.3.565. [DOI] [PubMed] [Google Scholar]
  16. Roth J. A., Kaeberle M. L. Evaluation of bovine polymorphonuclear leukocyte function. Vet Immunol Immunopathol. 1981 Apr;2(2):157–174. doi: 10.1016/0165-2427(81)90047-7. [DOI] [PubMed] [Google Scholar]
  17. Simmons S. R., Karnovsky M. L. Iodinating ability of various leukocytes and their bactericidal activity. J Exp Med. 1973 Jul 1;138(1):44–63. doi: 10.1084/jem.138.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Syuto B., Matsumoto M. Purification of a protective antigen from a saline extract of Pasteurella multocida. Infect Immun. 1982 Sep;37(3):1218–1226. doi: 10.1128/iai.37.3.1218-1226.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yost F. J., Jr, Fridovich I. Superoxide radicals and phagocytosis. Arch Biochem Biophys. 1974 Apr 2;161(2):395–401. doi: 10.1016/0003-9861(74)90320-8. [DOI] [PubMed] [Google Scholar]

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