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. 1984 Jan;43(1):391–396. doi: 10.1128/iai.43.1.391-396.1984

A small plasmid in Shigella dysenteriae 1 specifies one or more functions essential for O antigen production and bacterial virulence.

H Watanabe, K N Timmis
PMCID: PMC263439  PMID: 6360905

Abstract

The role of plasmids in the virulence of Shigella dysenteriae 1 W30864, which contains at least five species, was investigated. By means of a standard plasmid-curing procedure, that is, bacterial cultivation at an elevated temperature, five virulence-deficient derivatives were obtained. One of these lacked a small, 6-megadalton plasmid, designated pHW400, exhibited reduced invasiveness for HeLa cells, and failed to produce the somatic antigen. Transposon tagging of the pHW400 plasmid to produce pHW401 and the transfer of this derivative into a variant of strain W30864 lacking pHW400 confirmed the conclusion that the pHW400 plasmid encodes one or more functions involved in O antigen (lipopolysaccharide) biosynthesis and bacterial virulence. A plasmid of similar size was detected in all of the three other strains of S. dysenteriae 1 examined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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