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. 1993 Apr;31(4):994–997. doi: 10.1128/jcm.31.4.994-997.1993

Increased specificity of antibody detection in surgical patients with invasive candidiasis with cytoplasmic antigens depleted of mannan residues.

A J van Deventer 1, H J van Vliet 1, L Voogd 1, W C Hop 1, W H Goessens 1
PMCID: PMC263602  PMID: 8463408

Abstract

In this study, it was shown that the diagnostic accuracy of antibody detection by a counterimmunoelectrophoresis technique could be improved by using cytoplasmic antigens depleted of mannan residues. The specificity of the counterimmunoelectrophoresis increased from 28.6 to 78.6% when cytoplasmic antigens depleted of mannan were used, while the sensitivity slightly decreased from 80 to 70%.

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Selected References

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  1. Axelsen N. H., Kirkpatrick C. H., Buckley R. H. Precipitins to Candida albicans in chronic mucocutaneous candidiasis studied by crossed immunoelectrophoresis with intermediate gel. Correlation with clinical and immunological findings. Clin Exp Immunol. 1974 Jul;17(3):385–394. [PMC free article] [PubMed] [Google Scholar]
  2. Bailey J. W., Sada E., Brass C., Bennett J. E. Diagnosis of systemic candidiasis by latex agglutination for serum antigen. J Clin Microbiol. 1985 May;21(5):749–752. doi: 10.1128/jcm.21.5.749-752.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bisbe J., Miro J. M., Torres J. M., Latorre X., Alia C., Amaral M., Estivill D., Mallolas J., Trilla A., Soriano E. Diagnostic value of serum antibody and antigen detection in heroin addicts with systemic candidiasis. Rev Infect Dis. 1989 Mar-Apr;11(2):310–315. doi: 10.1093/clinids/11.2.310. [DOI] [PubMed] [Google Scholar]
  4. Burges G. E., Holley H. P., Jr, Burdash N. M., Virella G. Immunological diagnosis of systemic candidiasis by counterimmunoelectrophoresis and quantitative immunofluorescence using purified antigens. Diagn Immunol. 1983;1(4):295–302. [PubMed] [Google Scholar]
  5. Burnie J. P., Williams J. D. Evaluation of the Ramco latex agglutination test in the early diagnosis of systemic candidiasis. Eur J Clin Microbiol. 1985 Apr;4(2):98–101. doi: 10.1007/BF02013571. [DOI] [PubMed] [Google Scholar]
  6. Ellsworth J. H., Reiss E., Bradley R. L., Chmel H., Armstrong D. Comparative serological and cutaneous reactivity of candidal cytoplasmic proteins and mannan separated by affinity for concanavalin A. J Clin Microbiol. 1977 Jan;5(1):91–99. doi: 10.1128/jcm.5.1.91-99.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fisher J. F., Trincher R. C., Agel J. F., Buxton T. B., Walker C. A., Johnson D. H., Cormier R. E., Chew W. H., Rissing J. P. Disseminated candidiasis: a comparison of two immunologic techniques in the diagnosis. Am J Med Sci. 1985 Oct;290(4):135–142. doi: 10.1097/00000441-198510000-00001. [DOI] [PubMed] [Google Scholar]
  8. Fung J. C., Donta S. T., Tilton R. C. Candida detection system (CAND-TEC) to differentiate between Candida albicans colonization and disease. J Clin Microbiol. 1986 Oct;24(4):542–547. doi: 10.1128/jcm.24.4.542-547.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenfield R. A., Troutt D. L., Rickard R. C., Altmiller D. H. Comparison of antibody, antigen, and metabolite assays in rat models of systemic and gastrointestinal candidiasis. J Clin Microbiol. 1988 Mar;26(3):409–417. doi: 10.1128/jcm.26.3.409-417.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones G. R., Warnock D. W. Observations on the use of the double diffusion test in the diagnosis of vaginal candidiasis. J Clin Pathol. 1977 Mar;30(3):262–265. doi: 10.1136/jcp.30.3.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jones J. M. Laboratory diagnosis of invasive candidiasis. Clin Microbiol Rev. 1990 Jan;3(1):32–45. doi: 10.1128/cmr.3.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jones J. M. Quantitation of antibody against cell wall mannan and a major cytoplasmic antigen of Candida in rabbits, mice, and humans. Infect Immun. 1980 Oct;30(1):78–89. doi: 10.1128/iai.30.1.78-89.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kahn F. W., Jones J. M. Latex agglutination tests for detection of Candida antigens in sera of patients with invasive candidiasis. J Infect Dis. 1986 Mar;153(3):579–585. doi: 10.1093/infdis/153.3.579. [DOI] [PubMed] [Google Scholar]
  14. Kostiala I., Kostiala A. A., Larinkari U., Valtonen V. V., Miettinen A. Antibodies against antigens of Candida albicans in patients with fungaemia and bacteraemia, studied by ELISA, precipitation, passive haemagglutination and immunofluorescence techniques. J Med Microbiol. 1981 Nov;14(4):483–492. doi: 10.1099/00222615-14-4-483. [DOI] [PubMed] [Google Scholar]
  15. Lew M. A., Siber G. R., Donahue D. M., Maiorca F. Enhanced detection with an enzyme-linked immunosorbent assay of candida mannan in antibody-containing serum after heat extraction. J Infect Dis. 1982 Jan;145(1):45–56. doi: 10.1093/infdis/145.1.45. [DOI] [PubMed] [Google Scholar]
  16. Marier R., Andriole V. T. Comparison of continuous and discontinuous counterimmunoelectrophoresis with immunodiffusion in identification of candida antibody using HS antigen. J Clin Microbiol. 1978 Jul;8(1):12–14. doi: 10.1128/jcm.8.1.12-14.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Matthews R. C., Burnie J. P., Tabaqchali S. Immunoblot analysis of the serological response in systemic candidosis. Lancet. 1984 Dec 22;2(8417-8418):1415–1418. doi: 10.1016/s0140-6736(84)91618-0. [DOI] [PubMed] [Google Scholar]
  18. Matthews R., Burnie J. Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol. 1988 Mar;26(3):459–463. doi: 10.1128/jcm.26.3.459-463.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meckstroth K. L., Reiss E., Keller J. W., Kaufman L. Detection of antibodies and antigenemia in leukemic patients with candidiasis by enzyme-linked immunosorbent assay. J Infect Dis. 1981 Jul;144(1):24–32. doi: 10.1093/infdis/144.1.24. [DOI] [PubMed] [Google Scholar]
  20. Phillips P., Dowd A., Jewesson P., Radigan G., Tweeddale M. G., Clarke A., Geere I., Kelly M. Nonvalue of antigen detection immunoassays for diagnosis of candidemia. J Clin Microbiol. 1990 Oct;28(10):2320–2326. doi: 10.1128/jcm.28.10.2320-2326.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Platenkamp G. J., Van Duin A. M., Porsius J. C., Schouten H. J., Zondervan P. E., Michel M. F. Diagnosis of invasive candidiasis in patients with and without signs of immune deficiency: a comparison of six detection methods in human serum. J Clin Pathol. 1987 Oct;40(10):1162–1167. doi: 10.1136/jcp.40.10.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Porsius J. C., van Vliet H. J., van Zeijl J. H., Goessens W. H., Michel M. F. Detection of an antibody response in immunocompetent patients with systemic candidiasis or Candida albicans colonisation. Eur J Clin Microbiol Infect Dis. 1990 May;9(5):352–355. doi: 10.1007/BF01973744. [DOI] [PubMed] [Google Scholar]
  23. Syverson R. E., Buckley H. R., Gibian J. R. Increasing the predictive value positive of the precipitin test for the diagnosis of deep-seated candidiasis. Am J Clin Pathol. 1978 Nov;70(5):826–831. doi: 10.1093/ajcp/70.5.826. [DOI] [PubMed] [Google Scholar]
  24. Taschdjian C. L., Kozinn P. J., Fink H., Cuesta M. B., Caroline L., Kantrowitz A. B. Post mortem studies of systemic candidiasis: I. Diagnostic validity of precipitin reaction and probable origin of sensitization to cytoplasmic candidal antigens. Sabouraudia. 1969 Jun;7(2):110–117. [PubMed] [Google Scholar]
  25. Vanmali A. N., Lentnek A. L., Franco J., Ngwenya B. Z. Comparison of immunodiffusion and crossed-immunoelectrophoresis in the diagnosis of invasive candidiasis. Eur J Clin Microbiol. 1983 Jun;2(3):206–212. doi: 10.1007/BF02029517. [DOI] [PubMed] [Google Scholar]
  26. Zöller L., Krämer I., Kappe R., Sonntag H. G. Enzyme immunoassays for invasive Candida infections: reactivity of somatic antigens of Candida albicans. J Clin Microbiol. 1991 Sep;29(9):1860–1867. doi: 10.1128/jcm.29.9.1860-1867.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. de Repentigny L. Serological techniques for diagnosis of fungal infection. Eur J Clin Microbiol Infect Dis. 1989 Apr;8(4):362–375. doi: 10.1007/BF01963470. [DOI] [PubMed] [Google Scholar]

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