Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Apr;31(4):1009–1012. doi: 10.1128/jcm.31.4.1009-1012.1993

Rapid and highly reproducible method for antifungal susceptibility testing of Aspergillus species.

H Yamada 1, S Kohno 1, S Maesaki 1, H Koga 1, M Kaku 1, K Hara 1, H Tanaka 1
PMCID: PMC263607  PMID: 8463380

Abstract

A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by a reference method.

Full text

PDF
1010

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Brandsberg J. W., French M. E. In vitro susceptibility of isolates of Aspergillus fumigatus and Sporothrix schenckii to amphotericin B. Antimicrob Agents Chemother. 1972 Nov;2(5):402–404. doi: 10.1128/aac.2.5.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denning D. W., Hanson L. H., Perlman A. M., Stevens D. A. In vitro susceptibility and synergy studies of Aspergillus species to conventional and new agents. Diagn Microbiol Infect Dis. 1992 Jan;15(1):21–34. doi: 10.1016/0732-8893(92)90053-v. [DOI] [PubMed] [Google Scholar]
  4. Denning D. W., Tucker R. M., Hanson L. H., Stevens D. A. Treatment of invasive aspergillosis with itraconazole. Am J Med. 1989 Jun;86(6 Pt 2):791–800. doi: 10.1016/0002-9343(89)90475-0. [DOI] [PubMed] [Google Scholar]
  5. Gordon M. A., Lapa E. W., Passero P. G. Improved method for azole antifungal susceptibility testing. J Clin Microbiol. 1988 Sep;26(9):1874–1877. doi: 10.1128/jcm.26.9.1874-1877.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kitahara M., Seth V. K., Medoff G., Kobayashi G. S. Antimicrobial susceptibility testing of six clinical isolates of Aspergillus. Antimicrob Agents Chemother. 1976 Jun;9(6):908–914. doi: 10.1128/aac.9.6.908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Martinez J., Torres J. M., Arteaga F., Foz-Tena A. Determination of the minimal inhibitory concentration of amphotericin B and miconazole for 21 strains of Aspergillus. Mycopathologia. 1978 Nov 10;64(3):147–151. doi: 10.1007/BF00576365. [DOI] [PubMed] [Google Scholar]
  8. Odds F. C., Cheesman S. L., Abbott A. B. Antifungal effects of fluconazole (UK 49858), a new triazole antifungal, in vitro. J Antimicrob Chemother. 1986 Oct;18(4):473–478. doi: 10.1093/jac/18.4.473. [DOI] [PubMed] [Google Scholar]
  9. Plempel M., Büchel K. H., Bartmann K., Regel E. Antimycotic properties of clotrimazole. Postgrad Med J. 1974 Jul;50 (Suppl 1):11–12. [PubMed] [Google Scholar]
  10. Saag M. S., Dismukes W. E. Azole antifungal agents: emphasis on new triazoles. Antimicrob Agents Chemother. 1988 Jan;32(1):1–8. doi: 10.1128/aac.32.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schmitt H. J., Bernard E. M., Andrade J., Edwards F., Schmitt B., Armstrong D. MIC and fungicidal activity of terbinafine against clinical isolates of Aspergillus spp. Antimicrob Agents Chemother. 1988 May;32(5):780–781. doi: 10.1128/aac.32.5.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shadomy S. In vitro antifungal activity of clotrimazole (Bay b 5097). Infect Immun. 1971 Aug;4(2):143–148. doi: 10.1128/iai.4.2.143-148.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Van Cutsem J., Van Gerven F., Janssen P. A. Activity of orally, topically, and parenterally administered itraconazole in the treatment of superficial and deep mycoses: animal models. Rev Infect Dis. 1987 Jan-Feb;9 (Suppl 1):S15–S32. doi: 10.1093/clinids/9.supplement_1.s15. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES