Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1984 Jun;44(3):633–636. doi: 10.1128/iai.44.3.633-636.1984

Isotype of protective anti-Salmonella antibodies in experimental mouse salmonellosis.

H Saxén, O Mäkelä, S B Svenson
PMCID: PMC263652  PMID: 6202639

Abstract

Mice and a rabbit were immunized with heat-killed Salmonella typhimurium bacteria or with an O-4,12 antigen-specific octasaccharide-protein conjugate. Immunoglobulin isotypes of the antisera were tested for their capacity to protect mice against experimental salmonellosis. Antibodies of immunoglobulin M + A isotypes were more protective than the immunoglobulin G antibodies in each of the two pools of mouse sera. The same protective pattern was also seen with a rabbit antiserum elicited by the artificial octasaccharide-protein conjugate, i.e., with antibodies with the exclusive specificity for the O-4,12 antigen determinants of S. typhimurium.

Full text

PDF
634

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  2. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  3. Karch H., Gmeiner J., Nixdorff K. Alteration of the immunoglobulin G subclass responses in mice to lipopolysaccharide: effects of nonbacterial proteins and bacterial membrane phospholipids or outer membrane proteins of Proteus mirabilis. Infect Immun. 1983 Apr;40(1):157–165. doi: 10.1128/iai.40.1.157-165.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Karjalainen K., Mäkelä O. A mendelian idiotype is demonstrable in the heteroclitic anti-NP antibodies of the mouse. Eur J Immunol. 1978 Feb;8(2):105–111. doi: 10.1002/eji.1830080207. [DOI] [PubMed] [Google Scholar]
  5. Kuusi N., Nurminen M., Saxen H., Valtonen M., Mäkelä P. H. Immunization with major outer membrane proteins in experimental salmonellosis of mice. Infect Immun. 1979 Sep;25(3):857–862. doi: 10.1128/iai.25.3.857-862.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mackaness G. B., Blanden R. V., Collins F. M. Host-parasite relations in mouse typhoid. J Exp Med. 1966 Oct 1;124(4):573–583. doi: 10.1084/jem.124.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Metcalf E. S., O'Brien A. D. Characterization of murine antibody response to Salmonella typhimurium by a class-specific solid-phase radioimmunoassay. Infect Immun. 1981 Jan;31(1):33–41. doi: 10.1128/iai.31.1.33-41.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nikaido H., Levinthal M., Nikaido K., Nakane K. Extended deletions in the histidine-rough-B region of the Salmonella chromosome. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1825–1832. doi: 10.1073/pnas.57.6.1825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Péterfy F., Kuusela P., Mäkelä O. Affinity requirements for antibody assays mapped by monoclonal antibodies. J Immunol. 1983 Apr;130(4):1809–1813. [PubMed] [Google Scholar]
  10. Reed N. D., Manning J. K., Rudbach J. A. Immunologic responses of mice to lipopolysaccharide from Escherichia coli. J Infect Dis. 1973 Jul;128(Suppl):70–74. doi: 10.1093/infdis/128.supplement_1.s70. [DOI] [PubMed] [Google Scholar]
  11. Reif A. E. Batch preparation of rabbit gammaG globulin with deae-cellulose. Immunochemistry. 1969 Sep;6(5):723–731. doi: 10.1016/0019-2791(67)90136-x. [DOI] [PubMed] [Google Scholar]
  12. Rowley D., Turner K. J. Number of molecules of antibody required to promote phagocytosis of one bacterium. Nature. 1966 Apr 30;210(5035):496–498. doi: 10.1038/210496a0. [DOI] [PubMed] [Google Scholar]
  13. Saxen H., Mäkelä O. The protective capacity of immune sera in experimental mouse salmonellosis is mainly due to IgM antibodies. Immunol Lett. 1982 Nov;5(5):267–272. doi: 10.1016/0165-2478(82)90110-9. [DOI] [PubMed] [Google Scholar]
  14. Seppälä I., Sarvas H., Péterfy F., Mäkelä O. The four subclasses of IgG can be isolated from mouse serum by using Protein A-Sepharose. Scand J Immunol. 1981 Oct;14(4):335–342. doi: 10.1111/j.1365-3083.1981.tb00573.x. [DOI] [PubMed] [Google Scholar]
  15. Svenson S. B., Lindberg A. A. Coupling of acid labile Salmonella specific oligosaccharides to macromolecular carriers. J Immunol Methods. 1979;25(4):323–335. doi: 10.1016/0022-1759(79)90025-5. [DOI] [PubMed] [Google Scholar]
  16. Svenson S. B., Lindberg A. A. Protection against mouse typhoid by artificial Salmonella vaccines. Scand J Infect Dis Suppl. 1980;Suppl 24:210–215. [PubMed] [Google Scholar]
  17. Svenson S. B., Nurminen M., Lindberg A. A. Artificial Salmonella vaccines: O-antigenic oligosaccharide-protein conjugates induce protection against infection with Salmonella typhimurium. Infect Immun. 1979 Sep;25(3):863–872. doi: 10.1128/iai.25.3.863-872.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Valtonen V. V. Mouse virulence of Salmonella strains: the effect of different smooth-type O side-chains. J Gen Microbiol. 1970 Dec;64(3):255–268. doi: 10.1099/00221287-64-3-255. [DOI] [PubMed] [Google Scholar]
  19. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic respones to lipopolysaccharides. J Exp Med. 1974 Nov 1;140(5):1147–1161. doi: 10.1084/jem.140.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES