Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 May;32(5):1238–1245. doi: 10.1128/jcm.32.5.1238-1245.1994

Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice.

J G Fox 1, F E Dewhirst 1, J G Tully 1, B J Paster 1, L Yan 1, N S Taylor 1, M J Collins Jr 1, P L Gorelick 1, J M Ward 1
PMCID: PMC263656  PMID: 8051250

Abstract

A bacterium with a spiral shape and bipolar, single, sheathed flagella was isolated from the livers of mice with active, chronic hepatitis. The bacteria also colonized the cecal and colonic mucosae of mice. The bacterium grew at 37 degrees C under microaerophilic and anaerobic conditions, rapidly hydrolyzed urea, was catalase and oxidase positive, reduced nitrate to nitrite, and was resistant to cephalothin metronidazole. On the basis of 16S rRNA gene sequence analysis, the organism was classified as a novel helicobacter, Helicobacter hepaticus. This new helicobacter, like two other murine Helicobacter species, H. muridarum and "H. rappini," is an efficient colonizer of the gastrointestinal tract, but in addition, it has the pathogenic potential to elicit persistent hepatitis in mice.

Full text

PDF
1241

Images in this article

1240Image
on p.1240

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer J. R., Romero S., Ritchie A. E., Hamacher M. E., Steiner B. M., Bryner J. H., Schell R. F. Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol. 1988 Jan;26(1):101–105. doi: 10.1128/jcm.26.1.101-105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bronsdon M. A., Goodwin C. S., Sly L. I., Chilvers T., Schoenknecht F. D. Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina) Int J Syst Bacteriol. 1991 Jan;41(1):148–153. doi: 10.1099/00207713-41-1-148. [DOI] [PubMed] [Google Scholar]
  3. Bryner J. H., Ritchie A. E., Pollet L., Kirkbride C. A., Collins J. E. Experimental infection and abortion of pregnant guinea pigs with a unique spirillum-like bacterium isolated from aborted ovine fetuses. Am J Vet Res. 1987 Jan;48(1):91–95. [PubMed] [Google Scholar]
  4. Eaton K. A., Dewhirst F. E., Radin M. J., Fox J. G., Paster B. J., Krakowka S., Morgan D. R. Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int J Syst Bacteriol. 1993 Jan;43(1):99–106. doi: 10.1099/00207713-43-1-99. [DOI] [PubMed] [Google Scholar]
  5. Fennell C. L., Totten P. A., Quinn T. C., Patton D. L., Holmes K. K., Stamm W. E. Characterization of Campylobacter-like organisms isolated from homosexual men. J Infect Dis. 1984 Jan;149(1):58–66. doi: 10.1093/infdis/149.1.58. [DOI] [PubMed] [Google Scholar]
  6. Foster J. R. Bacterial infection of the common bile duct in chronic fascioliasis in the rat. J Comp Pathol. 1984 Apr;94(2):175–181. doi: 10.1016/0021-9975(84)90038-0. [DOI] [PubMed] [Google Scholar]
  7. Fox J. G., Blanco M., Murphy J. C., Taylor N. S., Lee A., Kabok Z., Pappo J. Local and systemic immune responses in murine Helicobacter felis active chronic gastritis. Infect Immun. 1993 Jun;61(6):2309–2315. doi: 10.1128/iai.61.6.2309-2315.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fox J. G., Correa P., Taylor N. S., Lee A., Otto G., Murphy J. C., Rose R. Helicobacter mustelae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans. Gastroenterology. 1990 Aug;99(2):352–361. doi: 10.1016/0016-5085(90)91016-y. [DOI] [PubMed] [Google Scholar]
  9. Fox J. G., Correa P., Taylor N. S., Zavala D., Fontham E., Janney F., Rodriguez E., Hunter F., Diavolitsis S. Campylobacter pylori-associated gastritis and immune response in a population at increased risk of gastric carcinoma. Am J Gastroenterol. 1989 Jul;84(7):775–781. [PubMed] [Google Scholar]
  10. Fox J. G., Edrise B. M., Cabot E. B., Beaucage C., Murphy J. C., Prostak K. S. Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res. 1986 Feb;47(2):236–239. [PubMed] [Google Scholar]
  11. Graham D. Y. Campylobacter pylori and peptic ulcer disease. Gastroenterology. 1989 Feb;96(2 Pt 2 Suppl):615–625. doi: 10.1016/s0016-5085(89)80057-5. [DOI] [PubMed] [Google Scholar]
  12. Kirkbride C. A., Gates C. E., Collins J. E., Ritchie A. E. Ovine abortion associated with an anaerobic bacterium. J Am Vet Med Assoc. 1985 Apr 15;186(8):789–791. [PubMed] [Google Scholar]
  13. Kita E., Katsui N., Nishi K., Emoto M., Yanagase Y., Kashiba S. Hepatic lesions in experimental Campylobacter jejuni infection of mice. J Gen Microbiol. 1986 Nov;132(11):3095–3103. doi: 10.1099/00221287-132-11-3095. [DOI] [PubMed] [Google Scholar]
  14. Kita E., Nishikawa F., Kamikaidou N., Nakano A., Katsui N., Kashiba S. Mononuclear cell response in the liver of mice infected with hepatotoxigenic Campylobacter jejuni. J Med Microbiol. 1992 Nov;37(5):326–331. doi: 10.1099/00222615-37-5-326. [DOI] [PubMed] [Google Scholar]
  15. Kita E., Oku D., Hamuro A., Nishikawa F., Emoto M., Yagyu Y., Katsui N., Kashiba S. Hepatotoxic activity of Campylobacter jejuni. J Med Microbiol. 1990 Nov;33(3):171–182. doi: 10.1099/00222615-33-3-171. [DOI] [PubMed] [Google Scholar]
  16. Kusukawa N., Uemori T., Asada K., Kato I. Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. Biotechniques. 1990 Jul;9(1):66-8, 70, 72. [PubMed] [Google Scholar]
  17. Lee A., Fox J., Hazell S. Pathogenicity of Helicobacter pylori: a perspective. Infect Immun. 1993 May;61(5):1601–1610. doi: 10.1128/iai.61.5.1601-1610.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lee A., Hazell S. L., O'Rourke J., Kouprach S. Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun. 1988 Nov;56(11):2843–2850. doi: 10.1128/iai.56.11.2843-2850.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lee A., Phillips M. W., O'Rourke J. L., Paster B. J., Dewhirst F. E., Fraser G. J., Fox J. G., Sly L. I., Romaniuk P. J., Trust T. J. Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol. 1992 Jan;42(1):27–36. doi: 10.1099/00207713-42-1-27. [DOI] [PubMed] [Google Scholar]
  20. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. doi: 10.1016/s0140-6736(84)91816-6. [DOI] [PubMed] [Google Scholar]
  21. Nomura A., Stemmermann G. N., Chyou P. H., Kato I., Perez-Perez G. I., Blaser M. J. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med. 1991 Oct 17;325(16):1132–1136. doi: 10.1056/NEJM199110173251604. [DOI] [PubMed] [Google Scholar]
  22. Olsen G. J., Overbeek R., Larsen N., Marsh T. L., McCaughey M. J., Maciukenas M. A., Kuan W. M., Macke T. J., Xing Y., Woese C. R. The Ribosomal Database Project. Nucleic Acids Res. 1992 May 11;20 (Suppl):2199–2200. doi: 10.1093/nar/20.suppl.2199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. doi: 10.1056/NEJM199110173251603. [DOI] [PubMed] [Google Scholar]
  24. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O'Rourke J. L., Taylor N. S., Ferrero R. Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int J Syst Bacteriol. 1991 Jan;41(1):31–38. doi: 10.1099/00207713-41-1-31. [DOI] [PubMed] [Google Scholar]
  25. Phillips M. W., Lee A. Isolation and characterization of a spiral bacterium from the crypts of rodent gastrointestinal tracts. Appl Environ Microbiol. 1983 Feb;45(2):675–683. doi: 10.1128/aem.45.2.675-683.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Queiroz D. M., Contigli C., Coimbra R. S., Nogueira A. M., Mendes E. N., Rocha G. A., Moura S. B. Spiral bacterium associated with gastric, ileal and caecal mucosa of mice. Lab Anim. 1992 Oct;26(4):288–294. doi: 10.1258/002367792780745760. [DOI] [PubMed] [Google Scholar]
  27. Romero S., Archer J. R., Hamacher M. E., Bologna S. M., Schell R. F. Case report of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol. 1988 Jan;26(1):142–143. doi: 10.1128/jcm.26.1.142-143.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
  29. Schauer D. B., Ghori N., Falkow S. Isolation and characterization of "Flexispira rappini" from laboratory mice. J Clin Microbiol. 1993 Oct;31(10):2709–2714. doi: 10.1128/jcm.31.10.2709-2714.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stanley J., Linton D., Burnens A. P., Dewhirst F. E., Owen R. J., Porter A., On S. L., Costas M. Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype. J Gen Microbiol. 1993 Oct;139(10):2495–2504. doi: 10.1099/00221287-139-10-2495. [DOI] [PubMed] [Google Scholar]
  31. Studier J. A., Keppler K. J. A note on the neighbor-joining algorithm of Saitou and Nei. Mol Biol Evol. 1988 Nov;5(6):729–731. doi: 10.1093/oxfordjournals.molbev.a040527. [DOI] [PubMed] [Google Scholar]
  32. Totten P. A., Fennell C. L., Tenover F. C., Wezenberg J. M., Perine P. L., Stamm W. E., Holmes K. K. Campylobacter cinaedi (sp. nov.) and Campylobacter fennelliae (sp. nov.): two new Campylobacter species associated with enteric disease in homosexual men. J Infect Dis. 1985 Jan;151(1):131–139. doi: 10.1093/infdis/151.1.131. [DOI] [PubMed] [Google Scholar]
  33. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991 Jan;41(1):88–103. doi: 10.1099/00207713-41-1-88. [DOI] [PubMed] [Google Scholar]
  34. Wotherspoon A. C., Doglioni C., Diss T. C., Pan L., Moschini A., de Boni M., Isaacson P. G. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet. 1993 Sep 4;342(8871):575–577. doi: 10.1016/0140-6736(93)91409-f. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES