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. 1994 Jul;32(7):1625–1628. doi: 10.1128/jcm.32.7.1625-1628.1994

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates.

M A Pfaller 1, Q Vu 1, M Lancaster 1, A Espinel-Ingroff 1, A Fothergill 1, C Grant 1, M R McGinnis 1, L Pasarell 1, M G Rinaldi 1, L Steele-Moore 1
PMCID: PMC263742  PMID: 7929747

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

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Selected References

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