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. 1994 Oct;32(10):2350–2352. doi: 10.1128/jcm.32.10.2350-2352.1994

Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR.

M Domeika 1, M Bassiri 1, P A Mårdh 1
PMCID: PMC264064  PMID: 7814464

Abstract

An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compared with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis in first-void urine (FVU) from 184 men attending a skin and venereal disease clinic. The prevalence of C. trachomatis in the population studied was 18.5%. Discrepant results between Syva EIA and Roche PCR were retested by using major outer membrane protein primer-based PCR. After retesting, the sensitivity, the specificity, and the positive and negative predictive values for the Syva EIA were 85.3, 100, 100, and 77.5%, respectively, and those for the Roche PCR 100, 100, 100, and 100%, respectively. It was concluded that PCR provides a highly sensitive and specific noninvasive screening method for genital chlamydial infection in asymptomatic men.

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Selected References

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