Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Dec;42(3):924–929. doi: 10.1128/iai.42.3.924-929.1983

Mapping of chromosomal genes that determine the El Tor biotype in Vibrio cholerae.

B A Green, J W Newland, R K Holmes
PMCID: PMC264387  PMID: 6315594

Abstract

The El Tor biotype of Vibrio cholerae has several characteristics that differentiate it from the classical biotype of V. cholerae. Among these are production of soluble hemolysin(s), a cell-associated hemagglutinin for chicken erythrocytes, resistance to polymyxin B, and resistance to bacteriophages of Mukerjee group IV. In the present study, we located the determinants for hemolysin (hly), chicken erythrocyte hemagglutinin (cha), and polymyxin B resistance (pmx) on the genetic map of V. cholerae. Transposon-facilitated recombination was used to perform conjugal matings between El Tor donor strains and classical recipient strains of V. cholerae. Recombinants were selected that had inherited specific nutritional markers from the donor strains and streptomycin resistance from the recipient strains. The recombinants were tested for the presence or absence of the unselected donor markers hly, cha, and pmx. These three El Tor biotype markers were found to be closely linked to each other and were located between the pyrA-201 and his-2 loci on the genetic map of V. cholerae.

Full text

PDF
927

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BHASKARAN K. Recombination of characters between mutant stocks of Vibrio cholerae, strain 162. J Gen Microbiol. 1960 Aug;23:47–54. doi: 10.1099/00221287-23-1-47. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bramucci M. G., Holmes R. K. Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae. J Clin Microbiol. 1978 Aug;8(2):252–255. doi: 10.1128/jcm.8.2.252-255.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Citarella R. V., Colwell R. R. Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J Bacteriol. 1970 Oct;104(1):434–442. doi: 10.1128/jb.104.1.434-442.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Finkelstein R. A., Atthasampunna P., Chulasamaya M., Charunmethee P. Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A. J Immunol. 1966 Mar;96(3):440–449. [PubMed] [Google Scholar]
  6. Holmgren J., Svennerholm A. M., Lindblad M. Receptor-like glycocompounds in human milk that inhibit classical and El Tor Vibrio cholerae cell adherence (hemagglutination). Infect Immun. 1983 Jan;39(1):147–154. doi: 10.1128/iai.39.1.147-154.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson S. R., Romig W. R. Transposon-facilitated recombination in Vibrio cholerae. Mol Gen Genet. 1979 Feb 16;170(1):93–101. doi: 10.1007/BF00268584. [DOI] [PubMed] [Google Scholar]
  8. Jones G. W., Abrams G. D., Freter R. Adhesive properties of Vibrio cholerae: adhesion to isolated rabbit brush border membranes and hemagglutinating activity. Infect Immun. 1976 Jul;14(1):232–239. doi: 10.1128/iai.14.1.232-239.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ogg J. E., Timme T. L., Alemohammad M. M. General Transduction in Vibrio cholerae. Infect Immun. 1981 Feb;31(2):737–741. doi: 10.1128/iai.31.2.737-741.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Parker C., Gauthier D., Tate A., Richardson K., Romig W. R. Expanded linkage map of Vibrio cholerae. Genetics. 1979 Feb;91(2):191–214. doi: 10.1093/genetics/91.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Parker C., Romig W. R. Self-transfer and genetic recombination mediated by P, the sex factor of Vibrio cholerae. J Bacteriol. 1972 Nov;112(2):707–714. doi: 10.1128/jb.112.2.707-714.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saunders D. W., Schanbacher K. J., Bramucci M. G. Mapping of a gene in Vibrio cholerae that determines the antigenic structure of cholera toxin. Infect Immun. 1982 Dec;38(3):1109–1116. doi: 10.1128/iai.38.3.1109-1116.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sublett R. D., Romig W. R. Transposon-facilitated recombination in classical biotypes of Vibrio cholerae. Infect Immun. 1981 Jun;32(3):1132–1138. doi: 10.1128/iai.32.3.1132-1138.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES