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. 1983 Dec;42(3):1191–1194. doi: 10.1128/iai.42.3.1191-1194.1983

Inhibition of keratinocyte proliferation by extracts of Actinobacillus actinomycetemcomitans.

P R Kamen
PMCID: PMC264425  PMID: 6196295

Abstract

A heat-labile sonic extract of a nonleukotoxic strain of Actinobacillus actinomycetemcomitans caused a dose-dependent inhibition of proliferation of human keratinocytes. Exposure of cells to heat-labile sonic extract for periods as brief as 10 min produced significant suppression of [3H]thymidine uptake 72 h later. I propose that alteration of epithelial barriers may be one pathogenic mechanism of this organism.

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Selected References

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  1. Baehni P., Tsai C. C., McArthur W. P., Hammond B. F., Taichman N. S. Interaction of inflammatory cells and oral microorganisms. VIII. Detection of leukotoxic activity of a plaque-derived gram-negative microorganism. Infect Immun. 1979 Apr;24(1):233–243. doi: 10.1128/iai.24.1.233-243.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Heijl L., Rifkin B. R., Zander H. A. Conversion of chronic gingivitis to periodontitis in squirrel monkeys. J Periodontol. 1976 Dec;47(12):710–716. doi: 10.1902/jop.1976.47.12.710. [DOI] [PubMed] [Google Scholar]
  3. Kamen P. R. The effects of bacterial sonicates on human keratinizing stratified squamous epithelium in vitro. J Periodontal Res. 1981 May;16(3):323–330. doi: 10.1111/j.1600-0765.1981.tb00981.x. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Nowotny A., Behling U. H., Hammond B., Lai C. H., Listgarten M., Pham P. H., Sanavi F. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun. 1982 Jul;37(1):151–154. doi: 10.1128/iai.37.1.151-154.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rheinwald J. G., Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975 Nov;6(3):331–343. doi: 10.1016/s0092-8674(75)80001-8. [DOI] [PubMed] [Google Scholar]
  7. Ryder M. I. Histological and ultrastructural characteristics of the periodontal syndrome in the rice rat. I. General light microscopic observations and ultrastructural observations of initial inflammatory changes. J Periodontal Res. 1980 Sep;15(5):502–515. doi: 10.1111/j.1600-0765.1980.tb00308.x. [DOI] [PubMed] [Google Scholar]
  8. SCHULTZ-HAUDT S., BIBBY B. G., BRUCE M. A. Tissue-destructive products of gingival bacteria from nonspecific gingivitis. J Dent Res. 1954 Oct;33(5):624–631. doi: 10.1177/00220345540330050601. [DOI] [PubMed] [Google Scholar]
  9. SCHULTZ-HAUDT S., DEWAR M., BIBBY B. G. Effects of hyaluronidase on human gingival epithelium. Science. 1953 Jun 12;117(3050):653–655. doi: 10.1126/science.117.3050.653. [DOI] [PubMed] [Google Scholar]
  10. Schroeder H. E., Lindhe J. Conversion of stable established gingivitis in the dog into destructive periodontitis. Arch Oral Biol. 1975 Dec;20(12):775–782. doi: 10.1016/0003-9969(75)90052-7. [DOI] [PubMed] [Google Scholar]
  11. Shenker B. J., Kushner M. E., Tsai C. C. Inhibition of fibroblast proliferation by Actinobacillus actinomycetemcomitans. Infect Immun. 1982 Dec;38(3):986–992. doi: 10.1128/iai.38.3.986-992.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shenker B. J., McArthur W. P., Tsai C. C. Immune suppression induced by Actinobacillus actinomycetemcomitans. I. Effects on human peripheral blood lymphocyte responses to mitogens and antigens. J Immunol. 1982 Jan;128(1):148–154. [PubMed] [Google Scholar]
  13. Thilander H. Epithelial changes in gingivitis. An electron microscopy study. J Periodontal Res. 1968;3(4):303–312. doi: 10.1111/j.1600-0765.1968.tb01936.x. [DOI] [PubMed] [Google Scholar]

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