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. 1983 Nov;42(2):558–566. doi: 10.1128/iai.42.2.558-566.1983

In vitro and in vivo complementation of Streptococcus mutans mutants defective in adherence.

S Larrimore, H Murchison, T Shiota, S M Michalek, R Curtiss 3rd
PMCID: PMC264464  PMID: 6642643

Abstract

Previous studies have shown that adherence-defective mutants of Streptococcus mutans PS14, serotype c, can be grouped into several different phenotypic groups. In this study a method was developed to test for complementation between pairs of nonadhering mutants which possess different genotypic defects. Mutant strains UAB95 and a spectinomycin-resistant derivative of UAB95 (UAB516) were found to exhibit increased levels of adherence when grown together with UAB230 in media containing sucrose as compared to the adherence of each strain grown separately. An increase in caries was also observed in gnotobiotic rats mixedly infected with the two mutants as compared to either strain alone. Tests revealed that UAB95 produced more water-insoluble glucan than its parent strain but had a defect in glucan binding. UAB230 was found to produce levels of a defective glucan that could not be bound by mutant or wild-type cells. Our results suggest that UAB95 produces a water-insoluble glucan which is bound by UAB230, thus allowing complementation for adherence and caries production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Charlton G., Fitzgerald D. B., Keyes P. H. Hydrogen ion activity in dental plaques of hamsters during metabolism of sucrose, glucose and fructose. Arch Oral Biol. 1971 Jun;16(6):655–661. doi: 10.1016/0003-9969(71)90069-0. [DOI] [PubMed] [Google Scholar]
  2. Clark W. B., Bammann L. L., Gibbons R. J. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun. 1978 Mar;19(3):846–853. doi: 10.1128/iai.19.3.846-853.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark W. B., Gibbons R. J. Influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of Streptococcus mutans 6715 to hydroxyapatite surfaces. Infect Immun. 1977 Nov;18(2):514–523. doi: 10.1128/iai.18.2.514-523.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibbons R. J., Banghart S. Induction of dental caries in gnotobiotic rats with a levan-forming streptococcus and a streptococcus isolated from subacute bacterial endocarditis. Arch Oral Biol. 1968 Mar;13(3):297–308. doi: 10.1016/0003-9969(68)90128-3. [DOI] [PubMed] [Google Scholar]
  5. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  6. Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hillman J. D. Lactate dehydrogenase mutants of Streptococcus mutans: isolation and preliminary characterization. Infect Immun. 1978 Jul;21(1):206–212. doi: 10.1128/iai.21.1.206-212.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirasawa M., Kiyono H., Shiota T., Hull R. A., Curtiss R., 3rd, Michalek S. M., McGhee J. R. Virulence of Streptococcus mutans: restoration of pathogenesis of a glucosyltransferase-defective mutant (C4). Infect Immun. 1980 Mar;27(3):915–921. doi: 10.1128/iai.27.3.915-921.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ikeda T., Shiota T., McGhee J. R., Otake S., Michalek S. M., Ochiai K., Hirasawa M., Sugimoto K. Virulence of Streptococcus mutans: comparison of the effects of a coupling sugar and sucrose on certain metabolic activities and cariogenicity. Infect Immun. 1978 Feb;19(2):477–480. doi: 10.1128/iai.19.2.477-480.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Krasse B., Carlsson J. Various types of streptococci and experimental caries in hamsters. Arch Oral Biol. 1970 Jan;15(1):25–32. doi: 10.1016/0003-9969(70)90142-1. [DOI] [PubMed] [Google Scholar]
  11. Krasse B., Edwardsson S., Svensson I., Trell L. Implantation of caries-inducing streptococci in the human oral cavity. Arch Oral Biol. 1967 Feb;12(2):231–236. doi: 10.1016/0003-9969(67)90042-8. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Loesche W. J., Rowan J., Straffon L. H., Loos P. J. Association of Streptococcus mutants with human dental decay. Infect Immun. 1975 Jun;11(6):1252–1260. doi: 10.1128/iai.11.6.1252-1260.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCabe M. M., Hamelik R. M., Smith E. E. Purification of dextran-binding protein from cariogenic Streptococcus mutans. Biochem Biophys Res Commun. 1977 Sep 9;78(1):273–278. doi: 10.1016/0006-291x(77)91250-5. [DOI] [PubMed] [Google Scholar]
  15. Michalek S. M., McGhee J. R., Navia J. M. Virulence of Streptococcus mutans: a sensitive method for evaluating cariogenicity in young gnotobiotic rats. Infect Immun. 1975 Jul;12(1):69–75. doi: 10.1128/iai.12.1.69-75.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Michalek S. M., McGhee J. R., Shiota T., Devenyns D. Low sucrose levels promote extensive Streptococcus mutans-induced dental caries. Infect Immun. 1977 May;16(2):712–714. doi: 10.1128/iai.16.2.712-714.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Michalek S. M., Shiota T., Ikeda T., Navia J. M., McGhee J. R. Virulence of Streptococcus mutans: biochemical and pathogenic characteristics of mutant isolates. Proc Soc Exp Biol Med. 1975 Nov;150(2):498–502. doi: 10.3181/00379727-150-39064. [DOI] [PubMed] [Google Scholar]
  18. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun. 1973 Oct;8(4):555–562. doi: 10.1128/iai.8.4.555-562.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. II. Nature of the binding site and the adsorption of dextran-levan synthetase enzymes on the cell-wall surface of the streptococcus. Infect Immun. 1974 Feb;9(2):419–429. doi: 10.1128/iai.9.2.419-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murchison H., Larrimore S., Curtiss R., 3rd Isolation and characterization of Streptococcus mutans mutants defective in adherence and aggregation. Infect Immun. 1981 Dec;34(3):1044–1055. doi: 10.1128/iai.34.3.1044-1055.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Otake S., Hirasawa M., Brown T. A., Kawabata Y., Kiyono H., Michalek S. M., McGhee J. R., Shiota T. Virulence of Streptococcus mutans: characterization of a serotype g antigen-defective mutants and its revertants. Infect Immun. 1981 Jan;31(1):151–159. doi: 10.1128/iai.31.1.151-159.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Russell R. R. Glucan-binding proteins of Streptococcus mutans serotype c. J Gen Microbiol. 1979 May;112(1):197–201. doi: 10.1099/00221287-112-1-197. [DOI] [PubMed] [Google Scholar]
  23. Rölla G., Iversen O. J., Bonesvoll P. Lipoteichoic acid - the key to the adhesiveness of sucrose grown Streptococcus mutans. Adv Exp Med Biol. 1978;107:607–617. doi: 10.1007/978-1-4684-3369-2_69. [DOI] [PubMed] [Google Scholar]
  24. Staat R. H., Langley S. D., Doyle R. J. Streptococcus mutans adherence: presumptive evidence for protein-mediated attachment followed by glucan-dependent cellular accumulation. Infect Immun. 1980 Feb;27(2):675–681. doi: 10.1128/iai.27.2.675-681.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tanzer J. M., Chassy B. M., Krichevsky M. I. Sucrose metabolism by Streptococcus mutans, SL-I. Biochim Biophys Acta. 1971 Feb 28;261(2):379–387. doi: 10.1016/0304-4165(72)90062-1. [DOI] [PubMed] [Google Scholar]
  26. Yamada T., Carlsson J. Regulation of lactate dehydrogenase and change of fermentation products in streptococci. J Bacteriol. 1975 Oct;124(1):55–61. doi: 10.1128/jb.124.1.55-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. de Stoppelaar J. D., König K. G., Plasschaert A. J., van der Hoeven J. S. Decreased cariogenicity of a mutant of Streptococcus mutans. Arch Oral Biol. 1971 Aug;16(8):971–975. doi: 10.1016/0003-9969(71)90186-5. [DOI] [PubMed] [Google Scholar]

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