Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Nov;42(2):675–682. doi: 10.1128/iai.42.2.675-682.1983

Molecular identification of surface protein antigens of Campylobacter jejuni.

S M Logan, T J Trust
PMCID: PMC264482  PMID: 6642648

Abstract

The technique of immunoblotting was used to identify the surface protein antigens of Campylobacter jejuni. Polyclonal antisera were raised in rabbits to formalinized cells of a typical human fecal isolate, C. jejuni VC74. Surface components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fractions analyzed included whole cell lysates, sarcosinate-extracted outer membranes, released outer membrane blebs (fragments), isolated flagella, 0.2 M glycine-hydrochloride (pH 2.2) extract, saline extract, and material released by osmotic shocking. The ability of the antisera to recognize corresponding antigens on other strains of thermophilic campylobacters and Campylobacter fetus was also determined. The results demonstrated that heat-labile antigenic specificity was conferred on C. jejuni VC74 by an outer membrane protein with an approximate molecular weight of 92,500. Both the major outer membrane protein and the flagella were immunogenic but did not confer either strain or species serospecificity on the strains tested. Another major antigen on thermophilic campylobacter cells was a surface protein with an approximate molecular weight of 31,000. This common antigen was preferentially removed by glycine extraction but was not detectable in outer membrane prepared by sarcosinate extraction.

Full text

PDF
678

Images in this article

677Image
on p.677
678Image
on p.678
679Image
on p.679
680Image
on p.680
680Image
on p.680

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butzler J. P., Dekeyser P., Detrain M., Dehaen F. Related vibrio in stools. J Pediatr. 1973 Mar;82(3):493–495. doi: 10.1016/s0022-3476(73)80131-3. [DOI] [PubMed] [Google Scholar]
  2. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Acta Paediatr Belg. 1979 Apr-Jun;32(2):89–94. [PubMed] [Google Scholar]
  3. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  4. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hofstra H., Dankert J. Antigenic cross-reactivity of major outer membrane proteins in enterobacteriaceae species. J Gen Microbiol. 1979 Apr;111(2):293–302. doi: 10.1099/00221287-111-2-293. [DOI] [PubMed] [Google Scholar]
  6. Hébert G. A., Hollis D. G., Weaver R. E., Steigerwalt A. G., McKinney R. M., Brenner D. J. Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence. J Clin Microbiol. 1983 Mar;17(3):529–538. doi: 10.1128/jcm.17.3.529-538.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jayaraman S., Muthukkaruppan V. R. Freund's complete adjuvant induces split tolerance to sheep erythrocytes in the lizard, Calotes versicolor. Dev Comp Immunol. 1982 Winter;6(1):175–180. doi: 10.1016/0145-305x(82)90020-9. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lauwers S., Vlaes L., Butzler J. P. Campylobacter serotyping a epidemiology. Lancet. 1981 Jan 17;1(8212):158–159. doi: 10.1016/s0140-6736(81)90748-0. [DOI] [PubMed] [Google Scholar]
  10. Lee D. R., Schnaitman C. A. Comparison of outer membrane porin proteins produced by Escherichia coli and Salmonella typhimurium. J Bacteriol. 1980 Jun;142(3):1019–1022. doi: 10.1128/jb.142.3.1019-1022.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lior H., Woodward D. L., Edgar J. A., LaRoche L. J. Serotyping by slide agglutination of Campylobacter jejuni and epidemiology. Lancet. 1981 Nov 14;2(8255):1103–1104. doi: 10.1016/s0140-6736(81)91293-9. [DOI] [PubMed] [Google Scholar]
  12. Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Logan S. M., Trust T. J. Outer membrane characteristics of Campylobacter jejuni. Infect Immun. 1982 Dec;38(3):898–906. doi: 10.1128/iai.38.3.898-906.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCoy E. C., Doyle D., Burda K., Corbeil L. B., Winter A. J. Superficial antigens of Campylobacter (Vibrio) fetus: characterization of antiphagocytic component. Infect Immun. 1975 Mar;11(3):517–525. doi: 10.1128/iai.11.3.517-525.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Overbeeke N., Van Scharrenburg G., Lugtenberg B. Antigenic relationships between pore proteins of Escherichia coli K12. Eur J Biochem. 1980 Sep;110(1):247–254. doi: 10.1111/j.1432-1033.1980.tb04862.x. [DOI] [PubMed] [Google Scholar]
  16. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rautelin H., Kosunen T. U. An acid extract as a common antigen in Campylobacter coli and Campylobacter jejuni strains. J Clin Microbiol. 1983 Apr;17(4):700–701. doi: 10.1128/jcm.17.4.700-701.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Skirrow M. B. Campylobacter enteritis - the first five years. J Hyg (Lond) 1982 Oct;89(2):175–184. doi: 10.1017/s0022172400070704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Syuto B., Matsumoto M. Purification of a protective antigen from a saline extract of Pasteurella multocida. Infect Immun. 1982 Sep;37(3):1218–1226. doi: 10.1128/iai.37.3.1218-1226.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES