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. 1983 Sep;41(3):942–949. doi: 10.1128/iai.41.3.942-949.1983

Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain.

S Normark, D Lark, R Hull, M Norgren, M Båga, P O'Hanley, G Schoolnik, S Falkow
PMCID: PMC264592  PMID: 6136465

Abstract

The uropathogenic strain Escherichia coli J96 mediates mannose-resistant hemagglutination owing to production of a digalactoside-binding adhesin. A cosmid clone from this strain has been isolated that, when harbored in E. coli K-12, expressed Pap pili and this adhesin (R. Hull et al., Infect. Immun. 33:933-938, 1981). By transposon mutagenesis and by the construction of a number of hybrid plasmid derivatives, we have demonstrated that about 8.5 kilobases of DNA is required to generate a mannose-resistant hemagglutination-positive phenotype in E. coli K-12 strain P678-54. The structural gene for the Pap pili monomer, papA, has been identified and mapped close to the promotor-proximal end of the Pap operon. Although strain P678-54 that harbored a Tn5 insertion within papA showed a mannose-resistant hemagglutination-positive phenotype, it was negative in a competitive enzyme-linked immunosorbent assay with anti-Pap pilus serum. This could mean that a Pap adhesin is encoded by a region on the Pap operon that is distinct from papA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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