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. 1983 Sep;41(3):1296–1301. doi: 10.1128/iai.41.3.1296-1301.1983

Monoclonal antibodies against colonization factor antigen I pili from enterotoxigenic Escherichia coli.

E A Worobec, P Shastry, W Smart, R Bradley, B Singh, W Paranchych
PMCID: PMC264639  PMID: 6136463

Abstract

Hybridomas secreting monoclonal antibodies directed against intact colonization factor antigen I pili have been produced by the fusion of spleen cells from immunized BALB/c mice with NS1/SP2 myeloma cells. The four monoclones with the highest antibody titer, as detected by enzyme-linked immunosorbant assay (ELISA), were chosen for antibody amplification by production of mouse ascitic fluid. These four were examined for antibody specificity by ELISA and immunoblot assays, using six different pilus types. Three of the four monoclonal isolates were specific for only colonization factor antigen I pili in both assays, whereas the remaining isolate showed a distinct cross-reactivity with K99 pili in the ELISA assay but not in immunoblot analysis. These results indicate that this monoclone may be recognizing a common structural element between the two adhesive pilus types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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