Abstract
The adhesive fimbrial antigen F12 from a strain of uropathogenic Escherichia coli has been isolated and characterized. The antigen was purified by ammonium sulfate precipitation and gel chromatography. The protein subunit of the F12 fimbria has a molecular weight of 18,200; the N-terminal amino acid sequence of the subunit shows close resemblance to that of the subunits of other F fimbriae and the type 1 fimbriae. We identified in these proteins a pattern of alternating conserved and variable amino acid residues which could indicate a special structural and functional feature.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Axelsen N. H., Bock E., Kroll J. Comparison of antigens: the reaction of 'identity'. Scand J Immunol Suppl. 1973;1:91–94. doi: 10.1111/j.1365-3083.1973.tb03785.x. [DOI] [PubMed] [Google Scholar]
- Chou P. Y., Fasman G. D. Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978;47:45–148. doi: 10.1002/9780470122921.ch2. [DOI] [PubMed] [Google Scholar]
- Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fader R. C., Duffy L. K., Davis C. P., Kurosky A. Purification and chemical characterization of type 1 pili isolated from Klebsiella pneumoniae. J Biol Chem. 1982 Mar 25;257(6):3301–3305. [PubMed] [Google Scholar]
- Hagberg L., Jodal U., Korhonen T. K., Lidin-Janson G., Lindberg U., Svanborg Edén C. Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981 Feb;31(2):564–570. doi: 10.1128/iai.31.2.564-570.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klemm P., Orskov I., Orskov F. F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects. Infect Immun. 1982 May;36(2):462–468. doi: 10.1128/iai.36.2.462-468.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klemm P., Poulsen F., Harboe M. K., Foltmann B. N-terminal amino acid sequences of pepsinogens from dogfish and seal and of bovine pepsinogen B. Acta Chem Scand B. 1976;30(10):979–984. doi: 10.3891/acta.chem.scand.30b-0979. [DOI] [PubMed] [Google Scholar]
- Klemm P. Primary structure of the CFA1 fimbrial protein from human enterotoxigenic Escherichia coli strains. Eur J Biochem. 1982 May 17;124(2):339–348. doi: 10.1111/j.1432-1033.1982.tb06597.x. [DOI] [PubMed] [Google Scholar]
- Klemm P. The complete amino-acid sequence of the K88 antigen, a fimbrial protein from Escherichia coli. Eur J Biochem. 1981 Jul;117(3):617–627. doi: 10.1111/j.1432-1033.1981.tb06382.x. [DOI] [PubMed] [Google Scholar]
- Orskov I., Ferencz A., Orskov F. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriated Escherichia coli. Lancet. 1980 Apr 19;1(8173):887–887. doi: 10.1016/s0140-6736(80)91396-3. [DOI] [PubMed] [Google Scholar]
- Orskov I., Orskov F., Birch-Andersen A. Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infect Immun. 1980 Feb;27(2):657–666. doi: 10.1128/iai.27.2.657-666.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orskov I., Orskov F., Birch-Andersen A., Kanamori M., Svanborg-Edén C. O, K, H and fimbrial antigens in Escherichia coli serotypes associated with pyelonephritis and cystitis. Scand J Infect Dis Suppl. 1982;33:18–25. [PubMed] [Google Scholar]
- Svanborg Edén C., Gotschlich E. C., Korhonen T. K., Leffler H., Schoolnik G. Aspects on structure and function of pili on uropathogenic Escherichia coli. Prog Allergy. 1983;33:189–202. doi: 10.1159/000318330. [DOI] [PubMed] [Google Scholar]
- Svanborg-Edén C., Svennerholm A. M. Secretory immunoglobulin A and G antibodies prevent adhesion of Escherichia coli to human urinary tract epithelial cells. Infect Immun. 1978 Dec;22(3):790–797. doi: 10.1128/iai.22.3.790-797.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weeke B. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures. Scand J Immunol Suppl. 1973;1:15–35. doi: 10.1111/j.1365-3083.1973.tb03776.x. [DOI] [PubMed] [Google Scholar]
- Weeke B. Crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:47–56. doi: 10.1111/j.1365-3083.1973.tb03778.x. [DOI] [PubMed] [Google Scholar]