Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1983 Apr;40(1):177–183. doi: 10.1128/iai.40.1.177-183.1983

Differential activation of resident macrophage subsets with two sources of lymphokine preparations.

D R Tabor, P H Saluk
PMCID: PMC264833  PMID: 6219956

Abstract

Resident murine macrophages were separated into subsets by Percoll density gradient centrifugation before treatment with lipopolysaccharide-stimulated lymphocytes or different lymphokine preparations. The lymphokines used were culture supernatants from lymphocytes obtained from lipopolysaccharide-injected mice or from purified protein derivative-treated lymphocytes from mice bearing an active BCG infection. The macrophage subsets were activated by the stimulated lymphocytes or lymphokine preparations to express C3b receptor-mediated ingestion or to inhibit the intracellular replication of Toxoplasma gondii or both. The results showed that the macrophage subsets were heterogeneous with respect to ingestion and T. gondii inhibition when activated with lipopolysaccharide-stimulated lymphocytes or lipopolysaccharide-derived lymphokines but were all homogeneous when activated with lymphokines from purified protein derivative-stimulated lymphocytes. When the macrophage subsets were allowed to remain in vitro for various times before lymphokine treatment, the relative pattern of subset activation changed when treated with lipopolysaccharide-derived lymphokines. In contrast, the macrophage subsets remained equally activated throughout the in vitro period when treated with the lymphokines from purified protein derivative-stimulated lymphocytes. The results suggested that functional macrophage heterogeneity depends not only on the nature of the activating signal but also on a state of receptivity of that signal by the macrophage population.

Full text

PDF
177

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lee K. C., Wong M., McIntyre D. Characterization of macrophage subpopulations responsive to activation by endotoxin and lymphokines. J Immunol. 1981 Jun;126(6):2474–2479. [PubMed] [Google Scholar]
  3. McIntrye J., Rowley D., Jenkin C. R. The functional heterogeneity of macrophages at the single cell level. Aust J Exp Biol Med Sci. 1967 Dec;45(6):675–680. doi: 10.1038/icb.1967.67. [DOI] [PubMed] [Google Scholar]
  4. Miller G. A., Campbell M. W., Hudson J. L. Separation of rat peritoneal macrophages into functionally distinct subclasses by centrifugal elutriation. J Reticuloendothel Soc. 1980 Feb;27(2):167–174. [PubMed] [Google Scholar]
  5. Murray H. W., Cohn Z. A. Macrophage oxygen-dependent antimicrobial activity. I. Susceptibility of Toxoplasma gondii to oxygen intermediates. J Exp Med. 1979 Oct 1;150(4):938–949. doi: 10.1084/jem.150.4.938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PERKINS E. H., LEONARD M. R. Specificity of phagocytosis as it may relate to antibody formation. J Immunol. 1963 Feb;90:228–237. [PubMed] [Google Scholar]
  7. Rice S. G., Fishman M. Functional and morphological heterogeneity among rabbit peritoneal macrophages. Cell Immunol. 1974 Mar 30;11(1-3):130–145. doi: 10.1016/0008-8749(74)90014-8. [DOI] [PubMed] [Google Scholar]
  8. Serio C., Gandour D. M., Walker W. S. Macrophage functional heterogeneity: evidence for different antibody-dependent effector cell activities and expression of Fc-receptors among macrophage subpopulations. J Reticuloendothel Soc. 1979 Feb;25(2):197–206. [PubMed] [Google Scholar]
  9. Tabor D. R., Saluk P. H. The functional heterogeneity of murine-resident macrophages to a chemotactic signal and induction of C3b-receptor-mediated ingestion. Immunol Lett. 1981 Dec;3(6):371–376. doi: 10.1016/0165-2478(81)90069-9. [DOI] [PubMed] [Google Scholar]
  10. Wing E. J., Gardner I. D., Ryning F. W., Remington J. S. Dissociation of effector functions in populations of activated macrophages. Nature. 1977 Aug 18;268(5621):642–644. doi: 10.1038/268642a0. [DOI] [PubMed] [Google Scholar]
  11. Wing E. J., Krahenbuhl J. L., Remington J. S. Studies of macrophage function during Trichinella spiralis infection in mice. Immunology. 1979 Mar;36(3):479–485. [PMC free article] [PubMed] [Google Scholar]
  12. Wrigley D. M., Saluk P. H. Induction of C3b-mediated phagocytosis in macrophages by distinct populations of lipopolysaccharide-stimulated lymphocytes. Infect Immun. 1981 Dec;34(3):780–786. doi: 10.1128/iai.34.3.780-786.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES