Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Feb;30(2):411–417. doi: 10.1128/jcm.30.2.411-417.1992

Presence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids.

M P Hayette 1, G Strecker 1, C Faille 1, D Dive 1, D Camus 1, D W Mackenzie 1, D Poulain 1
PMCID: PMC265070  PMID: 1537911

Abstract

In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA) involving neoglycolipids constructed with these residues (NGLO). Oligomannosides O released by mild alkaline degradation of the C. albicans cell wall phosphopeptidomannan (PPM) contained one to seven mannose residues, among which the quantitatively major components, mannobiose and mannotriose, were shown by 1H nuclear magnetic resonance to contain exclusively alpha (1-2) linkages. The pool of oligomannosides was converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin-layer chromatography. We have tested against these neoantigens, coated on ELISA plates, 15 pairs of sera corresponding to individual seroconversions observed in 15 patients during the course of a mycological and serological survey of candidiasis. For all patients, seroconversions resulted in an increased level of antibodies against NGLO. A significant correlation was observed between the results of ELISA-NGLO, ELISA involving the original PPM molecule, and routine antibody detection tests, indirect immunofluorescence assay, and cocounterimmunoelectrophoresis. These results therefore demonstrate the synthesis of human antibodies reactive with oligomannosides O constitutive of the C. albicans mannan molecule which have been previously described as exhibiting an inhibitory effect on human lymphocytic proliferation.

Full text

PDF
416

Images in this article

413Image
on p.413
415Image
on p.415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey J. W., Sada E., Brass C., Bennett J. E. Diagnosis of systemic candidiasis by latex agglutination for serum antigen. J Clin Microbiol. 1985 May;21(5):749–752. doi: 10.1128/jcm.21.5.749-752.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chew W. H., Theus T. L. Candida precipitins. J Immunol. 1967 Feb;98(2):220–224. [PubMed] [Google Scholar]
  3. Cohen R. E., Ballou C. E. Linkage and sequence analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopy. Biochemistry. 1980 Sep 2;19(18):4345–4358. doi: 10.1021/bi00559a031. [DOI] [PubMed] [Google Scholar]
  4. Domer J. E. Candida cell wall mannan: a polysaccharide with diverse immunologic properties. Crit Rev Microbiol. 1989;17(1):33–51. doi: 10.3109/10408418909105721. [DOI] [PubMed] [Google Scholar]
  5. Faille C., Michalski J. C., Strecker G., Mackenzie D. W., Camus D., Poulain D. Immunoreactivity of neoglycolipids constructed from oligomannosidic residues of the Candida albicans cell wall. Infect Immun. 1990 Nov;58(11):3537–3544. doi: 10.1128/iai.58.11.3537-3544.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fukazawa Y. Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser. 1989;47:37–62. [PubMed] [Google Scholar]
  7. Gentry L. O., Wilkinson I. D., Lea A. S., Price M. F. Latex agglutination test for detection of Candida antigen in patients with disseminated disease. Eur J Clin Microbiol. 1983 Apr;2(2):122–128. doi: 10.1007/BF02001577. [DOI] [PubMed] [Google Scholar]
  8. HASENCLEVER H. F., MITCHELL W. O. Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans. J Bacteriol. 1961 Oct;82:570–573. doi: 10.1128/jb.82.4.570-573.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kobayashi H., Shibata N., Mitobe H., Ohkubo Y., Suzuki S. Structural study of phosphomannan of yeast-form cells of Candida albicans J-1012 strain with special reference to application of mild acetolysis. Arch Biochem Biophys. 1989 Aug 1;272(2):364–375. doi: 10.1016/0003-9861(89)90230-0. [DOI] [PubMed] [Google Scholar]
  10. Kocourek J., Ballou C. E. Method for fingerprinting yeast cell wall mannans. J Bacteriol. 1969 Dec;100(3):1175–1181. doi: 10.1128/jb.100.3.1175-1181.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kogan G., Pavliak V., Masler L. Structural studies of mannans from the cell walls of the pathogenic yeasts Candida albicans serotypes A and B and Candida parapsilosis. Carbohydr Res. 1988 Feb 1;172(2):243–253. doi: 10.1016/s0008-6215(00)90858-9. [DOI] [PubMed] [Google Scholar]
  12. Lehmann P. F., Reiss E. Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients. Mycopathologia. 1980 Mar 17;70(2):89–93. doi: 10.1007/BF00443073. [DOI] [PubMed] [Google Scholar]
  13. Nakajima T., Ballou C. E. Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation. J Biol Chem. 1974 Dec 10;249(23):7679–7684. [PubMed] [Google Scholar]
  14. Okubo Y., Ichikawa T., Suzuki S. Relationship between phosphate content and immunochemical properties of subfractions of bakers' yeast mannan. J Bacteriol. 1978 Oct;136(1):63–68. doi: 10.1128/jb.136.1.63-68.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Podzorski R. P., Gray G. R., Nelson R. D. Different effects of native Candida albicans mannan and mannan-derived oligosaccharides on antigen-stimulated lymphoproliferation in vitro. J Immunol. 1990 Jan 15;144(2):707–716. [PubMed] [Google Scholar]
  16. Poulain D., Ayadi A., Fruit J. Détection d'un antigène témoin d'infection systémique à Candida à l'aide d'un anticorps monoclonal couplé à l' or colloïdal. Ann Biol Clin (Paris) 1987;45(5):565–572. [PubMed] [Google Scholar]
  17. Poulain D., Fruit J., Fournier L., Dei Cas E., Vernes A. Diagnosis of systemic candidiasis: application of co-counterimmunoelectrophoresis. Eur J Clin Microbiol. 1986 Aug;5(4):427–434. doi: 10.1007/BF02075699. [DOI] [PubMed] [Google Scholar]
  18. Poulain D., Hopwood V., Vernes A. Antigenic variability of Candida albicans. Crit Rev Microbiol. 1985;12(3):223–270. doi: 10.3109/10408418509104430. [DOI] [PubMed] [Google Scholar]
  19. Poulain D., Tronchin G., Dubremetz J. F., Biguet J. Ultrastructure of the cell wall of Candida albicans blastospores: study of its constitutive layers by the use of a cytochemical technique revealing polysaccharides. Ann Microbiol (Paris) 1978 Feb-Mar;129(2):141–153. [PubMed] [Google Scholar]
  20. Shibata N., Fukasawa S., Kobayashi H., Tojo M., Yonezu T., Ambo A., Ohkubo Y., Suzuki S. Structural analysis of phospho-D-mannan-protein complexes isolated from yeast and mold form cells of Candida albicans NIH A-207 serotype A strain. Carbohydr Res. 1989 Apr 15;187(2):239–253. doi: 10.1016/0008-6215(89)80006-0. [DOI] [PubMed] [Google Scholar]
  21. Shibata N., Ichikawa T., Tojo M., Takahashi M., Ito N., Okubo Y., Suzuki S. Immunochemical study on the mannans of Candida albicans NIH A-207, NIH B-792, and J-1012 strains prepared by fractional precipitation with cetyltrimethylammonium bromide. Arch Biochem Biophys. 1985 Dec;243(2):338–348. doi: 10.1016/0003-9861(85)90511-9. [DOI] [PubMed] [Google Scholar]
  22. Shibata N., Kobayashi H., Tojo M., Suzuki S. Characterization of phosphomannan-protein complexes isolated from viable cells of yeast and mycelial forms of Candida albicans NIH B-792 strain by the action of Zymolyase-100T. Arch Biochem Biophys. 1986 Dec;251(2):697–708. doi: 10.1016/0003-9861(86)90379-6. [DOI] [PubMed] [Google Scholar]
  23. Suzuki M., Fukazawa Y. Immunochemical characterization of Candida albicans cell wall antigens: specific determinant of Candida albicans serotype A mannan. Microbiol Immunol. 1982;26(5):387–402. doi: 10.1111/j.1348-0421.1982.tb00189.x. [DOI] [PubMed] [Google Scholar]
  24. Tsuchiya T., Fukazawa Y., Taguchi M., Nakase T., Shinoda T. Serologic aspects on yeast classification. Mycopathol Mycol Appl. 1974 Aug 30;53(1):77–91. doi: 10.1007/BF02127199. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES