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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Feb;30(2):522–524. doi: 10.1128/jcm.30.2.522-524.1992

Comparison of four techniques for detection of antibodies to cytomegalovirus.

Y J Kraat 1, R M Hendrix 1, M P Landini 1, C A Bruggeman 1
PMCID: PMC265094  PMID: 1311337

Abstract

Four serological methods were compared and evaluated for use in detecting cytomegalovirus antibody in blood and organ donors. Western blotting (immunoblotting), latex agglutination, enzyme-linked immunosorbent assay, and a recent available microparticle enzyme immunosorbent assay were used. The microparticle enzyme immunoassay appears to compare favorably with each of the other three assays tested for screening blood and organ donors for a previous cytomegalovirus infection.

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Selected References

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  1. Adler S. P., McVoy M., Biro V. G., Britt W. J., Hider P., Marshall D. Detection of cytomegalovirus antibody with latex agglutination. J Clin Microbiol. 1985 Jul;22(1):68–70. doi: 10.1128/jcm.22.1.68-70.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adler S. P. Transfusion-associated cytomegalovirus infections. Rev Infect Dis. 1983 Nov-Dec;5(6):977–993. doi: 10.1093/clinids/5.6.977. [DOI] [PubMed] [Google Scholar]
  3. Barkholt L. M., Ericzon B. G., Ehrnst A., Forsgren M., Andersson J. P. Cytomegalovirus infections in liver transplant patients: incidence and outcome. Transplant Proc. 1990 Feb;22(1):235–237. [PubMed] [Google Scholar]
  4. Bowden R. A., Sayers M., Flournoy N., Newton B., Banaji M., Thomas E. D., Meyers J. D. Cytomegalovirus immune globulin and seronegative blood products to prevent primary cytomegalovirus infection after marrow transplantation. N Engl J Med. 1986 Apr 17;314(16):1006–1010. doi: 10.1056/NEJM198604173141602. [DOI] [PubMed] [Google Scholar]
  5. Braun D. K., Pereira L., Norrild B., Roizman B. Application of denatured, electrophoretically separated, and immobilized lysates of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins. J Virol. 1983 Apr;46(1):103–112. doi: 10.1128/jvi.46.1.103-112.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fox A. S., Tolpin M. D., Baker A. L., Broelsch C. E., Whittington P. F., Jackson T., Thistlethwaite J. R., Stuart F. P. Seropositivity in liver transplant recipients as a predictor of cytomegalovirus disease. J Infect Dis. 1988 Feb;157(2):383–385. doi: 10.1093/infdis/157.2.383. [DOI] [PubMed] [Google Scholar]
  7. Gray J. J., Alvey B., Smith D. J., Wreghitt T. G. Evaluation of a commercial latex agglutination test for detecting antibodies to cytomegalovirus in organ donors and transplant recipients. J Virol Methods. 1987 May;16(1-2):13–19. doi: 10.1016/0166-0934(87)90026-7. [DOI] [PubMed] [Google Scholar]
  8. Landini M. P., Mirolo G., Coppolecchia P., Re M. C., La Placa M. Serum antibodies to individual cytomegalovirus structural polypeptides in renal transplant recipients during viral infection. Microbiol Immunol. 1986;30(7):683–695. doi: 10.1111/j.1348-0421.1986.tb02994.x. [DOI] [PubMed] [Google Scholar]
  9. Landini M. P., Re M. C., Mirolo G., Baldassarri B., La Placa M. Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol. 1985 Dec;17(4):303–311. doi: 10.1002/jmv.1890170403. [DOI] [PubMed] [Google Scholar]
  10. McHugh T. M., Casavant C. H., Wilber J. C., Stites D. P. Comparison of six methods for the detection of antibody to cytomegalovirus. J Clin Microbiol. 1985 Dec;22(6):1014–1019. doi: 10.1128/jcm.22.6.1014-1019.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Metselaar H. J., Weimar W. Cytomegalovirus infection and renal transplantation. J Antimicrob Chemother. 1989 Jun;23 (Suppl E):37–47. doi: 10.1093/jac/23.suppl_e.37. [DOI] [PubMed] [Google Scholar]
  12. van Loon A. M., Heessen F. W., van der Logt J. T., van der Veen J. Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus. J Clin Microbiol. 1981 Mar;13(3):416–422. doi: 10.1128/jcm.13.3.416-422.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van Loon A. M., van der Logt J. T., van der Veen J. Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution. J Clin Pathol. 1981 Jun;34(6):665–669. doi: 10.1136/jcp.34.6.665. [DOI] [PMC free article] [PubMed] [Google Scholar]

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