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. 1992 Mar;30(3):633–641. doi: 10.1128/jcm.30.3.633-641.1992

Multicenter evaluation of five commercial rubella virus immunoglobulin G kits which report in international units per milliliter.

W Dimech 1, A Bettoli 1, D Eckert 1, B Francis 1, J Hamblin 1, T Kerr 1, C Ryan 1, I Skurrie 1
PMCID: PMC265124  PMID: 1551980

Abstract

In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precision coefficients of variation ranging from 5.1 to 21.7% and from 9.5 to 51.0%, respectively. To compare the international units expressed, the results from 40 samples tested in duplicate were compared with the results of a reference enzyme immunoassay calibrated with World Health Organization international standard serum and a hemagglutination inhibition test. The results of the kits were plotted against those of the reference tests, and linear regression analysis was applied. The Pearson correlation coefficient ranged from 0.64 to 0.75 when the commercial kit results were compared with those of the reference enzyme immunoassay, indicating only a moderate degree of correlation. Therefore, the international units expressed by the commercial kits are insufficiently consistent to be of practical use in diagnostic clinical microbiology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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