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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Apr;30(4):862–865. doi: 10.1128/jcm.30.4.862-865.1992

Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts.

P Kielstein 1, V J Rapp-Gabrielson 1
PMCID: PMC265175  PMID: 1572971

Abstract

Previous independent investigations of the serotyping of Haemophilus parasuis strains have led to the designation of serovars A to D, 1 to 7, Jena 6 to Jena 12, and ND1 to ND5. Heat-stable antigen preparations from the reference strains for these serovars were tested by immunodiffusion with rabbit hyperimmune antisera. The existence of 15 distinct serologic groups was apparent, for which we propose the designations serovars 1 to 15. Examination of 290 field isolates from swine in the former German Democratic Republic indicated a prevalence of serovars 4 and 5, which together accounted for 41% of the isolates examined. However, 26.2% of the isolates were nontypeable with this test procedure and available antisera. Intraperitoneal inoculation of specific-pathogen-free pigs with cells representing the 15 serovars indicated differences in virulence which may be serovar related. Cells of strains representing serovars 1, 5, 10, 12, 13, and 14 were the most virulent, causing death or moribundity in inoculated pigs. Cells of serovars 2, 4, 8, and 15 caused polyserositis, but not death, in inoculated pigs. However, inoculation of cells of strains representing serovars 3, 6, 7, 9, and 11 resulted in no clinical symptoms or lesions indicative of H. parasuis infection.

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Selected References

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  1. Baehler J. F., Burgisser H., de Meuron P. A., Nicolet J. Infection à Haemophilus parasuis chez le porc. Schweiz Arch Tierheilkd. 1974 Apr;116(4):183–188. [PubMed] [Google Scholar]
  2. Kielstein P., Rosner H., Müller W. Typing of heat-stable soluble Haemophilus parasuis antigen by means of agargel precipitation and the dot-blot procedure. Zentralbl Veterinarmed B. 1991 Jun;38(4):315–320. doi: 10.1111/j.1439-0450.1991.tb00877.x. [DOI] [PubMed] [Google Scholar]
  3. Miniats O. P., Smart N. L., Rosendal S. Cross protection among Haemophilus parasuis strains in immunized gnotobiotic pigs. Can J Vet Res. 1991 Jan;55(1):37–41. [PMC free article] [PubMed] [Google Scholar]
  4. Morozumi T., Nicolet J. Morphological variations of Haemophilus parasuis strains. J Clin Microbiol. 1986 Jan;23(1):138–142. doi: 10.1128/jcm.23.1.138-142.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morozumi T., Nicolet J. Some antigenic properties of Haemophilus parasuis and a proposal for serological classification. J Clin Microbiol. 1986 Jun;23(6):1022–1025. doi: 10.1128/jcm.23.6.1022-1025.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Møller K., Kilian M. V factor-dependent members of the family Pasteurellaceae in the porcine upper respiratory tract. J Clin Microbiol. 1990 Dec;28(12):2711–2716. doi: 10.1128/jcm.28.12.2711-2716.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nielsen R., Danielsen V. An outbreak of Glässer's disease. Studies on etiology, serology and the effect of vaccination. Nord Vet Med. 1975 Jan;27(1):20–25. [PubMed] [Google Scholar]
  8. Nielsen R. New diagnostic techniques: a review of the HAP group of bacteria. Can J Vet Res. 1990 Apr;54 (Suppl):S68–S72. [PubMed] [Google Scholar]
  9. Rosner H., Kielstein P., Müller W., Rohrmann B. Beziehungen zwischen Serotyp, Virulenz und SDS-PAGE-Proteinmustern von Haemophilus parasuis. Dtsch Tierarztl Wochenschr. 1991 Sep;98(9):327–330. [PubMed] [Google Scholar]
  10. Schimmel D., Kielstein P., Hass R. Zur serologische Typisierung von Haemophilus parasuis. Arch Exp Veterinarmed. 1985 Nov;39(6):944–947. [PubMed] [Google Scholar]
  11. Smart N. L., Miniats O. P., MacInnes J. I. Analysis of Haemophilus parasuis isolates from southern Ontario swine by restriction endonuclease fingerprinting. Can J Vet Res. 1988 Jul;52(3):319–324. [PMC free article] [PubMed] [Google Scholar]
  12. Smart N. L., Miniats O. P., Rosendal S., Friendship R. M. Glasser's disease and prevalence of subclinical infection with Haemophilus parasuis in swine in southern Ontario. Can Vet J. 1989 Apr;30(4):339–343. [PMC free article] [PubMed] [Google Scholar]

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