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. 1992 May;30(5):1120–1126. doi: 10.1128/jcm.30.5.1120-1126.1992

Identification of subgroups of bovine respiratory syncytial virus.

J C Baker 1, E G Wilson 1, G L McKay 1, R J Stanek 1, W J Underwood 1, L F Velicer 1, M A Mufson 1
PMCID: PMC265235  PMID: 1583108

Abstract

The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using MAbs and polyclonal antisera to HRSV and BRSV. Shared epitopes were demonstrated on four of five structural proteins between BRSV and both subgroups A and B of HRSV. The nine isolates of BRSV showed different patterns of reactivity in enzyme immunoassays with panels of MAbs to HRSV subgroups A and B. Major variations in the molecular weights of the P (phosphoprotein) and F (fusion protein) proteins were demonstrated among the BRSV isolates tested. These results suggest that BRSV belongs to a different antigenic grouping than HRSV and that BRSV is composed of two distinct subgroups.

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Selected References

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