Abstract
A new medium for detection of urease activity and isolation of Helicobacter pylori is proposed. This medium, containing Columbia Agar Base, was supplemented with IsoVitaleX, hemin, urea, and phenol red (nonselective medium [NSM]). Both bacterial growth and color change were evaluated and compared with growth in the same medium supplemented with cefsulodin, vancomycin, polymyxin B sulfate, and amphotericin B (selective medium [SM]). Twenty-five recent clinical isolates and antral biopsy specimens from 33 patients who underwent endoscopy were examined. The isolates showed a rapid color change and good growth at 5 days of incubation with NSM and SM. H. pylori-positive biopsies revealed a color change within 36 h, and bacterial growth was better appreciated in NSM, but with more contaminating flora than in SM.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coudron P. E., Kirby D. F. Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori. J Clin Microbiol. 1989 Jul;27(7):1527–1530. doi: 10.1128/jcm.27.7.1527-1530.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dent J. C., McNulty C. A. Evaluation of a new selective medium for Campylobacter pylori. Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):555–558. doi: 10.1007/BF01962615. [DOI] [PubMed] [Google Scholar]
- Drumm B., Sherman P. Long-term storage of Campylobacter pylori. J Clin Microbiol. 1989 Jul;27(7):1655–1656. doi: 10.1128/jcm.27.7.1655-1656.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldie J., Veldhuyzen van Zanten S. J., Jalali S., Hollingsworth J., Riddell R. H., Richardson H., Hunt R. H. Optimization of a medium for the rapid urease test for detection of Campylobacter pylori in gastric antral biopsies. J Clin Microbiol. 1989 Sep;27(9):2080–2082. doi: 10.1128/jcm.27.9.2080-2082.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Megraud F., Bonnet F., Garnier M., Lamouliatte H. Characterization of "Campylobacter pyloridis" by culture, enzymatic profile, and protein content. J Clin Microbiol. 1985 Dec;22(6):1007–1010. doi: 10.1128/jcm.22.6.1007-1010.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ormand J. E., Talley N. J., Shorter R. G., Conley C. R., Carpenter H. A., Fich A., Wilson W. R., Phillips S. F. Prevalence of Helicobacter pylori in specific forms of gastritis. Further evidence supporting a pathogenic role for H. pylori in chronic nonspecific gastritis. Dig Dis Sci. 1991 Feb;36(2):142–145. doi: 10.1007/BF01300747. [DOI] [PubMed] [Google Scholar]
- Peterson W. L. Helicobacter pylori and peptic ulcer disease. N Engl J Med. 1991 Apr 11;324(15):1043–1048. doi: 10.1056/NEJM199104113241507. [DOI] [PubMed] [Google Scholar]
- Sjöstedt S. The upper gastrointestinal microflora in relation to gastric diseases and gastric surgery. Acta Chir Scand Suppl. 1989;551:1–57. [PubMed] [Google Scholar]