Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Jun;30(6):1518–1524. doi: 10.1128/jcm.30.6.1518-1524.1992

Comparison of DNA fingerprints and somatic serotypes of serogroup B and E Pasteurella multocida isolates.

M A Wilson 1, R B Rimler 1, L J Hoffman 1
PMCID: PMC265321  PMID: 1378060

Abstract

The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. Fifty-four serogroup B isolates (isolated from classical cases of hemorrhagic septicemia) reacted with somatic serotype 2 or 5 antiserum and had DNA fingerprint profiles which resembled that of the serotype 2 reference strain. Seven DNA fingerprint profiles were found among 16 serogroup B strains representing other somatic serotypes. The DNA fingerprints of these isolates were different from the fingerprints of the 16 somatic reference serotype strains. All 13 serogroup E isolates had identical somatic serotypes and identical DNA fingerprint profiles when the HhaI endonuclease was used. The HhaI fingerprint profile of the serogroup E isolates did not match any fingerprint profile of the reference somatic serotype strains. Following DNA profiling with the HhaI endonuclease, the 13 serogroup E isolates were differentiated sequentially with HpaII restriction endonuclease. A descriptive identification epithet for P. multocida isolates was constructed. The descriptive epithet consists of serologic identification and sequential DNA profiles with restriction endonucleases HhaI and HpaII, respectively. DNA fingerprinting of P. multocida is a precise characterization method. In conjunction with serologic typing, it can further classify P. multocida isolates for epidemiologic studies.

Full text

PDF
1524

Images in this article

1521Image
on p.1521
1522Image
on p.1522
1522Image
on p.1522
1523Image
on p.1523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhasin J. L. Serological types of Pasteurella multocida isolated from turkeys and chickens in Canada. Can J Microbiol. 1982 Sep;28(9):1078–1080. doi: 10.1139/m82-160. [DOI] [PubMed] [Google Scholar]
  2. CARTER G. R. Studies on Pasteurella multocida. I. A hemagglutination test for the identification of serological types. Am J Vet Res. 1955 Jul;16(60):481–484. [PubMed] [Google Scholar]
  3. Harel J., Côté S., Jacques M. Restriction endonuclease analysis of porcine Pasteurella multocida isolates from Quebec. Can J Vet Res. 1990 Oct;54(4):422–426. [PMC free article] [PubMed] [Google Scholar]
  4. Heddleston K. L., Gallagher J. E., Rebers P. A. Fowl cholera: gel diffusion precipitin test for serotyping Pasteruella multocida from avian species. Avian Dis. 1972 Jul-Sep;16(4):925–936. [PubMed] [Google Scholar]
  5. Kim C. J., Nagaraja K. V. DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys. Am J Vet Res. 1990 Feb;51(2):207–210. [PubMed] [Google Scholar]
  6. Lu Y. S., Pakes S. P., Stefanu C. Capsular and somatic serotypes of Pasteurella multocida isolates recovered from healthy and diseased rabbits in Texas. J Clin Microbiol. 1983 Aug;18(2):292–295. doi: 10.1128/jcm.18.2.292-295.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pijoan C., Morrison R. B., Hilley H. D. Serotyping of Pasteurella multocida isolated from swine lungs collected at slaughter. J Clin Microbiol. 1983 Jun;17(6):1074–1076. doi: 10.1128/jcm.17.6.1074-1076.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rimler R. B., Brogden K. A. Pasteurella multocida isolated from rabbits and swine: serologic types and toxin production. Am J Vet Res. 1986 Apr;47(4):730–737. [PubMed] [Google Scholar]
  9. Rimler R. B. Comparisons of Pasteurella multocida lipopolysaccharides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine relationship between group B and E hemorrhagic septicemia strains and serologically related group A strains. J Clin Microbiol. 1990 Apr;28(4):654–659. doi: 10.1128/jcm.28.4.654-659.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rimler R. B., Rhoades K. R. Serogroup F, a new capsule serogroup of Pasteurella multocida. J Clin Microbiol. 1987 Apr;25(4):615–618. doi: 10.1128/jcm.25.4.615-618.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Snipes K. P., Hirsh D. C., Kasten R. W., Carpenter T. E., Hird D. W., McCapes R. H. Differentiation of field isolates of Pasteurella multocida serotype 3,4 from live vaccine strain by genotypic characterization. Avian Dis. 1990 Apr-Jun;34(2):419–424. [PubMed] [Google Scholar]
  12. Snipes K. P., Hirsh D. C., Kasten R. W., Carpenter T. E., Hird D. W., McCapes R. H. Homogeneity of characteristics of Pasteurella multocida isolated from turkeys and wildlife in California, 1985-88. Avian Dis. 1990 Apr-Jun;34(2):315–320. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES