Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Jul;30(7):1642–1645. doi: 10.1128/jcm.30.7.1642-1645.1992

Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms.

S H Goh 1, S K Byrne 1, J L Zhang 1, A W Chow 1
PMCID: PMC265357  PMID: 1352784

Abstract

Staphylocoagulase, a major phenotypic determinant of Staphylococcus aureus, exists in multiple allelic forms, in part because of the existence of gene variants within the 3'-end coding region. This region contains a series of repeating 81-bp DNA sequences which differ both in the number of tandem repeats and the location of AluI restriction sites among different isolates. Utilizing this finding, we developed a novel typing method for S. aureus based on polymerase chain reaction amplification of the variable region of the coagulase gene followed by AluI restriction enzyme digestion and analysis of restriction fragment length polymorphism (RFLP). Among 30 S. aureus isolates studied initially, a total of 10 distinct RFLP patterns were observed. There was excellent correlation of the RFLP patterns with typing of these isolates by multilocus enzyme electrophoresis at 20 chromosomal loci. This coagulase RFLP method was used to analyze an additional 39 S. aureus isolates and successfully traced the source of an outbreak of methicillin-resistant S. aureus infections at a local hospital.

Full text

PDF
1645

Images in this article

1643Image
on p.1643
1643Image
on p.1643

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bjorvatn B., Lund V., Kristiansen B. E., Korsnes L., Spanne O., Lindqvist B. Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis. J Clin Microbiol. 1984 Jun;19(6):763–765. doi: 10.1128/jcm.19.6.763-765.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HENDERSON A., BRODIE J. INVESTIGATIONS ON STAPHYLOCOCCAL COAGULASE. Br J Exp Pathol. 1963 Oct;44:524–528. [PMC free article] [PubMed] [Google Scholar]
  3. Hall L. M., Jordens J. Z., Wang F. Methicillin-resistant Staphylococcus aureus from China characterized by digestion of total DNA with restriction enzymes. Epidemiol Infect. 1989 Aug;103(1):183–192. doi: 10.1017/s095026880003048x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ichiyama S., Ohta M., Shimokata K., Kato N., Takeuchi J. Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1991 Dec;29(12):2690–2695. doi: 10.1128/jcm.29.12.2690-2695.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaida S., Miyata T., Yoshizawa Y., Igarashi H., Iwanaga S. Nucleotide and deduced amino acid sequences of staphylocoagulase gene from Staphylococcus aureus strain 213. Nucleic Acids Res. 1989 Nov 11;17(21):8871–8871. doi: 10.1093/nar/17.21.8871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaida S., Miyata T., Yoshizawa Y., Kawabata S., Morita T., Igarashi H., Iwanaga S. Nucleotide sequence of the staphylocoagulase gene: its unique COOH-terminal 8 tandem repeats. J Biochem. 1987 Nov;102(5):1177–1186. doi: 10.1093/oxfordjournals.jbchem.a122156. [DOI] [PubMed] [Google Scholar]
  7. Kaneko S., Feinstone S. M., Miller R. H. Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique. J Clin Microbiol. 1989 Sep;27(9):1930–1933. doi: 10.1128/jcm.27.9.1930-1933.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kawabata S., Morita T., Miyata T., Iwanaga S., Igarashi H. Isolation and characterization of staphylocoagulase chymotryptic fragment. Localization of the procoagulant- and prothrombin-binding domain of this protein. J Biol Chem. 1986 Jan 25;261(3):1427–1433. [PubMed] [Google Scholar]
  9. Matthews R., Burnie J. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ. 1989 Feb 11;298(6670):354–357. doi: 10.1136/bmj.298.6670.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Musser J. M., Hauser A. R., Kim M. H., Schlievert P. M., Nelson K., Selander R. K. Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2668–2672. doi: 10.1073/pnas.88.7.2668. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Musser J. M., Schlievert P. M., Chow A. W., Ewan P., Kreiswirth B. N., Rosdahl V. T., Naidu A. S., Witte W., Selander R. K. A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. Proc Natl Acad Sci U S A. 1990 Jan;87(1):225–229. doi: 10.1073/pnas.87.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phonimdaeng P., O'Reilly M., Nowlan P., Bramley A. J., Foster T. J. The coagulase of Staphylococcus aureus 8325-4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol. 1990 Mar;4(3):393–404. doi: 10.1111/j.1365-2958.1990.tb00606.x. [DOI] [PubMed] [Google Scholar]
  13. Reeves M. W., Drummond M. C., Tager M. Partial purification and characterization of the multiple molecular forms of staphylococcal clotting activity (coagulase). J Bacteriol. 1981 Dec;148(3):861–868. doi: 10.1128/jb.148.3.861-868.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Renaud F., Freney J., Etienne J., Bes M., Brun Y., Barsotti O., Andre S., Fleurette J. Restriction endonuclease analysis of Staphylococcus epidermidis DNA may be a useful epidemiological marker. J Clin Microbiol. 1988 Sep;26(9):1729–1734. doi: 10.1128/jcm.26.9.1729-1734.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tompkins L. S., Troup N. J., Woods T., Bibb W., McKinney R. M. Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis. J Clin Microbiol. 1987 Oct;25(10):1875–1880. doi: 10.1128/jcm.25.10.1875-1880.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tveten Y., Kristiansen B. E., Ask E., Jenkins A., Hofstad T. DNA fingerprinting of isolates of Staphylococcus aureus from newborns and their contacts. J Clin Microbiol. 1991 Jun;29(6):1100–1105. doi: 10.1128/jcm.29.6.1100-1105.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES