Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Sep;30(9):2279–2283. doi: 10.1128/jcm.30.9.2279-2283.1992

Development of oligonucleotide primers and probes against structural and regulatory genes of human immunodeficiency virus type 1 (HIV-1) and their use for amplification of HIV-1 provirus by using polymerase chain reaction.

M R Dawood 1, R Allan 1, K Fowke 1, J Embree 1, G W Hammond 1
PMCID: PMC265492  PMID: 1400991

Abstract

The polymerase chain reaction is a powerful technique for amplifying a few copies of double-stranded genetic material to millions of copies in a few hours. The sensitivity and specificity of the polymerase chain reaction technique depend to some extent on the nucleotide sequences of the oligonucleotide primer pair used in the amplification. We report new oligonucleotide primers and probes which can be used for the amplification and detection of human immunodeficiency virus type 1 provirus sequences of not only structural but also regulatory genes. These primers are very sensitive and specific and can be used for the detection of African and North American strains of human immunodeficiency virus type 1.

Full text

PDF
2280

Images in this article

2282Image
on p.2282
2282Image
on p.2282

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell J., Ratner L. Specificity of polymerase chain amplification reactions for human immunodeficiency virus type 1 DNA sequences. AIDS Res Hum Retroviruses. 1989 Feb;5(1):87–95. doi: 10.1089/aid.1989.5.87. [DOI] [PubMed] [Google Scholar]
  2. Chadwick E. G., Yogev R., Kwok S., Sninsky J. J., Kellogg D. E., Wolinsky S. M. Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pediatric patients. J Infect Dis. 1989 Dec;160(6):954–959. doi: 10.1093/infdis/160.6.954. [DOI] [PubMed] [Google Scholar]
  3. Chayt K. J., Harper M. E., Marselle L. M., Lewin E. B., Rose R. M., Oleske J. M., Epstein L. G., Wong-Staal F., Gallo R. C. Detection of HTLV-III RNA in lungs of patients with AIDS and pulmonary involvement. JAMA. 1986 Nov 7;256(17):2356–2359. [PubMed] [Google Scholar]
  4. Davis G. R., Blumeyer K., DiMichele L. J., Whitfield K. M., Chappelle H., Riggs N., Ghosh S. S., Kao P. M., Fahy E., Kwoh D. Y. Detection of human immunodeficiency virus type 1 in AIDS patients using amplification-mediated hybridization analyses: reproducibility and quantitative limitations. J Infect Dis. 1990 Jul;162(1):13–20. doi: 10.1093/infdis/162.1.13. [DOI] [PubMed] [Google Scholar]
  5. Edwards J. R., Ulrich P. P., Weintrub P. S., Cowan M. J., Levy J. A., Wara D. W., Vyas G. N. Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children. J Pediatr. 1989 Aug;115(2):200–203. doi: 10.1016/s0022-3476(89)80065-4. [DOI] [PubMed] [Google Scholar]
  6. Falk L. A., Jr, Paul D., Landay A., Kessler H. HIV isolation from plasma of HIV-infected persons. N Engl J Med. 1987 Jun 11;316(24):1547–1548. doi: 10.1056/NEJM198706113162413. [DOI] [PubMed] [Google Scholar]
  7. Folks T. M., Powell D., Lightfoote M., Koenig S., Fauci A. S., Benn S., Rabson A., Daugherty D., Gendelman H. E., Hoggan M. D. Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus. J Exp Med. 1986 Jul 1;164(1):280–290. doi: 10.1084/jem.164.1.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gallo R. C., Salahuddin S. Z., Popovic M., Shearer G. M., Kaplan M., Haynes B. F., Palker T. J., Redfield R., Oleske J., Safai B. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science. 1984 May 4;224(4648):500–503. doi: 10.1126/science.6200936. [DOI] [PubMed] [Google Scholar]
  9. Grankvist O., Gustafsson A., Bredberg-Rådén U., Pallangyo K., Mhalu F., Biberfeld P., Biberfeld G., Wadell G. Selection of primers of optimal sensitivity for the detection of HIV-1 from Africa and Europe by polymerase chain reaction. AIDS. 1991 May;5(5):575–578. [PubMed] [Google Scholar]
  10. Hart C., Schochetman G., Spira T., Lifson A., Moore J., Galphin J., Sninsky J., Ou C. Y. Direct detection of HIV RNA expression in seropositive subjects. Lancet. 1988 Sep 10;2(8611):596–599. doi: 10.1016/s0140-6736(88)90639-3. [DOI] [PubMed] [Google Scholar]
  11. Jehuda-Cohen T., Slade B. A., Powell J. D., Villinger F., De B., Folks T. M., McClure H. M., Sell K. W., Ahmed-Ansari A. Polyclonal B-cell activation reveals antibodies against human immunodeficiency virus type 1 (HIV-1) in HIV-1-seronegative individuals. Proc Natl Acad Sci U S A. 1990 May;87(10):3972–3976. doi: 10.1073/pnas.87.10.3972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koenig S., Gendelman H. E., Orenstein J. M., Dal Canto M. C., Pezeshkpour G. H., Yungbluth M., Janotta F., Aksamit A., Martin M. A., Fauci A. S. Detection of AIDS virus in macrophages in brain tissue from AIDS patients with encephalopathy. Science. 1986 Sep 5;233(4768):1089–1093. doi: 10.1126/science.3016903. [DOI] [PubMed] [Google Scholar]
  13. Kwok S., Mack D. H., Mullis K. B., Poiesz B., Ehrlich G., Blair D., Friedman-Kien A., Sninsky J. J. Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection. J Virol. 1987 May;61(5):1690–1694. doi: 10.1128/jvi.61.5.1690-1694.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lifson A. R., Stanley M., Pane J., O'Malley P. M., Wilber J. C., Stanley A., Jeffery B., Rutherford G. W., Sohmer P. R. Detection of human immunodeficiency virus DNA using the polymerase chain reaction in a well-characterized group of homosexual and bisexual men. J Infect Dis. 1990 Mar;161(3):436–439. doi: 10.1093/infdis/161.3.436. [DOI] [PubMed] [Google Scholar]
  15. Loche M., Mach B. Identification of HIV-infected seronegative individuals by a direct diagnostic test based on hybridisation to amplified viral DNA. Lancet. 1988 Aug 20;2(8608):418–421. doi: 10.1016/s0140-6736(88)90412-6. [DOI] [PubMed] [Google Scholar]
  16. Michaelis B. A., Levy J. A. Recovery of human immunodeficiency virus from serum. JAMA. 1987 Mar 13;257(10):1327–1327. doi: 10.1001/jama.1987.03390100065011. [DOI] [PubMed] [Google Scholar]
  17. Ou C. Y., Kwok S., Mitchell S. W., Mack D. H., Sninsky J. J., Krebs J. W., Feorino P., Warfield D., Schochetman G. DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science. 1988 Jan 15;239(4837):295–297. doi: 10.1126/science.3336784. [DOI] [PubMed] [Google Scholar]
  18. Rayfield M., De Cock K., Heyward W., Goldstein L., Krebs J., Kwok S., Lee S., McCormick J., Moreau J. M., Odehouri K. Mixed human immunodeficiency virus (HIV) infection in an individual: demonstration of both HIV type 1 and type 2 proviral sequences by using polymerase chain reaction. J Infect Dis. 1988 Dec;158(6):1170–1176. doi: 10.1093/infdis/158.6.1170. [DOI] [PubMed] [Google Scholar]
  19. Robert-Guroff M., Popovic M., Gartner S., Markham P., Gallo R. C., Reitz M. S. Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages. J Virol. 1990 Jul;64(7):3391–3398. doi: 10.1128/jvi.64.7.3391-3398.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rogers M. F., Ou C. Y., Rayfield M., Thomas P. A., Schoenbaum E. E., Abrams E., Krasinski K., Selwyn P. A., Moore J., Kaul A. Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group. N Engl J Med. 1989 Jun 22;320(25):1649–1654. doi: 10.1056/NEJM198906223202503. [DOI] [PubMed] [Google Scholar]
  21. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  22. Sheppard H. W., Ascher M. S., Busch M. P., Sohmer P. R., Stanley M., Luce M. C., Chimera J. A., Madej R., Rodgers G. C., Lynch C. A multicenter proficiency trial of gene amplification (PCR) for the detection of HIV-1. J Acquir Immune Defic Syndr. 1991;4(3):277–283. [PubMed] [Google Scholar]
  23. Wolinsky S. M., Rinaldo C. R., Kwok S., Sninsky J. J., Gupta P., Imagawa D., Farzadegan H., Jacobson L. P., Grovit K. S., Lee M. H. Human immunodeficiency virus type 1 (HIV-1) infection a median of 18 months before a diagnostic western blot. Evidence from a cohort of homosexual men. Ann Intern Med. 1989 Dec 15;111(12):961–972. doi: 10.7326/0003-4819-111-12-961. [DOI] [PubMed] [Google Scholar]
  24. Zagury D., Fouchard M., Vol J. C., Cattan A., Leibowitch J., Feldman M., Sarin P. S., Gallo R. C. Detection of infectious HTLV-III/LAV virus in cell-free plasma from AIDS patients. Lancet. 1985 Aug 31;2(8453):505–506. doi: 10.1016/s0140-6736(85)90442-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES