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. 1993 Jul;31(7):1764–1769. doi: 10.1128/jcm.31.7.1764-1769.1993

Hepatitis C markers in hemodialysis patients.

C S Huang 1, M S Ho 1, C S Yang 1, C L Lee 1, C A Tan 1
PMCID: PMC265628  PMID: 7688754

Abstract

The prevalence of hepatitis C virus (HCV) infection among the patients of a hemodialysis unit in Taiwan was determined by an immunoblot and reverse transcriptase-polymerase chain reaction algorithm to be 58.8% (67 of 114 patients) after serological surveys with two advanced-generation enzyme-linked immunosorbent assays (ELISAs) for anti-HCV and a C 100-3 single-antigen test. The results of the second-generation ELISAs, the supplementary immunoblot test, and the test for HCV RNA were in good agreement with each other, from 86.0 to 98.2%. The first-generation C 100-3 test lacked the sensitivity of the four other systems. The two advanced-generation screening ELISAs for anti-HCV, a multiple-recombinant-antigen test, the Abbott second-generation ELISA, and a synthetic peptide multiple-antigen test, the UBI HCV EIA, provided reliable and virtually equivalent detection of potentially infected blood. Antibodies to capsid 1 and capsid 2 determinants of the Liatek immunoblot system were the most frequently detected reactivities to HCV in the HCV-infected hemodialysis patients. The percentage of HCV-infected patients with abnormal liver function (alanine aminotransferase level, greater than 100 IU/liter) was higher than that of the uninfected patients. The prevalence of HCV infection was correlated to the duration of hemodialysis treatment and the amount of blood transfused, and the most common transmission mode was thought to be patient-to-patient transmission through the dialysis equipment. Several means of reducing the frequency of transmission between hemodialysis patients are suggested.

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Selected References

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  1. Aach R. D., Stevens C. E., Hollinger F. B., Mosley J. W., Peterson D. A., Taylor P. E., Johnson R. G., Barbosa L. H., Nemo G. J. Hepatitis C virus infection in post-transfusion hepatitis. An analysis with first- and second-generation assays. N Engl J Med. 1991 Nov 7;325(19):1325–1329. doi: 10.1056/NEJM199111073251901. [DOI] [PubMed] [Google Scholar]
  2. Abe K., Inchauspe G., Shikata T., Prince A. M. Three different patterns of hepatitis C virus infection in chimpanzees. Hepatology. 1992 Apr;15(4):690–695. doi: 10.1002/hep.1840150423. [DOI] [PubMed] [Google Scholar]
  3. Alonso M. C., Novoa D., Romero R., Arcocha V., Sanchez-Guisande D. Antibodies to hepatitis C virus in patients on haemodialysis. Nephron. 1991;57(2):247–247. doi: 10.1159/000186265. [DOI] [PubMed] [Google Scholar]
  4. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q. L., Kuo G. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N Engl J Med. 1989 Nov 30;321(22):1494–1500. doi: 10.1056/NEJM198911303212202. [DOI] [PubMed] [Google Scholar]
  5. Arici C., Gregis G. P., Marchesi D., Mingardi G., Mecca G., Bellavita P. Effectiveness of a preventive programme for non-A, non-B hepatitis in a large dialysis unit. Nephrol Dial Transplant. 1990;5(10):902–903. doi: 10.1093/ndt/5.10.902. [DOI] [PubMed] [Google Scholar]
  6. Boudart D., Lucas J. C., Adjou C., Muller J. Y. HCV confirmatory testing of blood donors. Lancet. 1992 Feb 8;339(8789):372–372. doi: 10.1016/0140-6736(92)91695-5. [DOI] [PubMed] [Google Scholar]
  7. Calabrese G., Vagelli G., Guaschino R., Gonella M. Transmission of anti-HCV within the household of haemodialysis patients. Lancet. 1991 Dec 7;338(8780):1466–1466. doi: 10.1016/0140-6736(91)92774-v. [DOI] [PubMed] [Google Scholar]
  8. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  9. Claeys H., Volckaerts A., De Beenhouwer H., Vermylen C. Association of hepatitis C virus carrier state with the occurrence of hepatitis C virus core antibodies. J Med Virol. 1992 Apr;36(4):259–264. doi: 10.1002/jmv.1890360405. [DOI] [PubMed] [Google Scholar]
  10. Esteban J. I., Esteban R., Viladomiu L., López-Talavera J. C., González A., Hernández J. M., Roget M., Vargas V., Genescà J., Buti M. Hepatitis C virus antibodies among risk groups in Spain. Lancet. 1989 Aug 5;2(8658):294–297. doi: 10.1016/s0140-6736(89)90485-6. [DOI] [PubMed] [Google Scholar]
  11. Farci P., Alter H. J., Wong D., Miller R. H., Shih J. W., Jett B., Purcell R. H. A long-term study of hepatitis C virus replication in non-A, non-B hepatitis. N Engl J Med. 1991 Jul 11;325(2):98–104. doi: 10.1056/NEJM199107113250205. [DOI] [PubMed] [Google Scholar]
  12. Gilli P., Moretti M., Soffritti S., Marchi N., Malacarne F., Bedani P. L., De Paoli Vitali E., Fiocchi O., Menini C. Non-A, non-B hepatitis and anti-HCV antibodies in dialysis patients. Int J Artif Organs. 1990 Nov;13(11):737–741. [PubMed] [Google Scholar]
  13. Ha S. K., Park J. H., Choi W. C., Kim K. H., Choi K. H., Lee H. Y., Han D. S., Song K. S., Kim H. O., Chung S. H. Hepatitis C infection in hemodialysis units. Korean J Intern Med. 1990 Jul;5(2):83–86. doi: 10.3904/kjim.1990.5.2.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jeffers L. J., Perez G. O., de Medina M. D., Ortiz-Interian C. J., Schiff E. R., Reddy K. R., Jimenez M., Bourgoignie J. J., Vaamonde C. A., Duncan R. Hepatitis C infection in two urban hemodialysis units. Kidney Int. 1990 Aug;38(2):320–322. doi: 10.1038/ki.1990.203. [DOI] [PubMed] [Google Scholar]
  15. Kaneko S., Unoura M., Kobayashi K., Kuno K., Murakami S., Hattori N. Detection of serum hepatitis C virus RNA. Lancet. 1990 Apr 21;335(8695):976–976. doi: 10.1016/0140-6736(90)91042-9. [DOI] [PubMed] [Google Scholar]
  16. Kudesia G., Chapman S., Makris M., Preston F. E. Need for second-generation anti-HCV testing in haemophilia. Lancet. 1992 Feb 22;339(8791):501–502. doi: 10.1016/0140-6736(92)91120-w. [DOI] [PubMed] [Google Scholar]
  17. Kuo G., Choo Q. L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21;244(4902):362–364. doi: 10.1126/science.2496467. [DOI] [PubMed] [Google Scholar]
  18. Lazizi Y., Elfassi E., Pillot J. Detection of hepatitis C virus sequences in sera with controversial serology by nested polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):931–934. doi: 10.1128/jcm.30.4.931-934.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lee S. D., Tsai Y. T., Hwang S. J., Wu J. C., Yung C. H., Cheng K. K., Lo K. J. A prospective study of post-transfusion non-A, non-B (type C) hepatitis following cardiovascular surgery in Taiwan. J Med Virol. 1991 Mar;33(3):188–192. doi: 10.1002/jmv.1890330309. [DOI] [PubMed] [Google Scholar]
  20. Lenzi M., Johnson P. J., McFarlane I. G., Ballardini G., Smith H. M., McFarlane B. M., Bridger C., Vergani D., Bianchi F. B., Williams R. Antibodies to hepatitis C virus in autoimmune liver disease: evidence for geographical heterogeneity. Lancet. 1991 Aug 3;338(8762):277–280. doi: 10.1016/0140-6736(91)90418-o. [DOI] [PubMed] [Google Scholar]
  21. Marchesi D., Arici C., Poletti E., Mingardi G., Minola E., Mecca G. Outbreak of non-A, non-B hepatitis in centre haemodialysis patients: a retrospective analysis. Nephrol Dial Transplant. 1988;3(6):795–799. [PubMed] [Google Scholar]
  22. McHutchison J. G., Person J. L., Govindarajan S., Valinluck B., Gore T., Lee S. R., Nelles M., Polito A., Chien D., DiNello R. Improved detection of hepatitis C virus antibodies in high-risk populations. Hepatology. 1992 Jan;15(1):19–25. doi: 10.1002/hep.1840150105. [DOI] [PubMed] [Google Scholar]
  23. Moroni G. A., Cori P., Marelli F., Del Prete M., Padovese P., Gallieni M., Anelli A., Brancaccio D. Indirect evidence for transfusion role in conditioning hepatitis C virus prevalence among dialysis patients. Nephron. 1991;57(3):371–372. doi: 10.1159/000186292. [DOI] [PubMed] [Google Scholar]
  24. Oguchi H., Terashima M., Tokunaga S., Kiyosawa K., Tanaka E., Furuta S., Kanno Y., Aizawa T., Nishioka K. Prevalence of anti-HCV in patients on long-term hemodialysis. Nihon Jinzo Gakkai Shi. 1990 Mar;32(3):313–317. [PubMed] [Google Scholar]
  25. Okamoto H., Okada S., Sugiyama Y., Tanaka T., Sugai Y., Akahane Y., Machida A., Mishiro S., Yoshizawa H., Miyakawa Y. Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region. Jpn J Exp Med. 1990 Aug;60(4):215–222. [PubMed] [Google Scholar]
  26. Ponz E., Campistol J. M., Bruguera M., Barrera J. M., Gil C., Pinto J. B., Andreu J. Hepatitis C virus infection among kidney transplant recipients. Kidney Int. 1991 Oct;40(4):748–751. doi: 10.1038/ki.1991.270. [DOI] [PubMed] [Google Scholar]
  27. Roger S. D., Crewe E., Cunningham A., Harris D. C. Hepatitis C virus infection in haemodialysis patients. Aust N Z J Med. 1991 Feb;21(1):22–24. doi: 10.1111/j.1445-5994.1991.tb02996.x. [DOI] [PubMed] [Google Scholar]
  28. Tsai S. J., Chen C. J., Hsieh Y. S., Yang C. S. Seroprevalence of anti-HCV among voluntary blood donors in Taiwan. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1991 Feb;24(1):61–70. [PubMed] [Google Scholar]
  29. Wang J. T., Wang T. H., Lin J. T., Sheu J. C., Sung J. L., Chen D. S. Hepatitis C virus in a prospective study of posttransfusion non-A, non-B hepatitis in Taiwan. J Med Virol. 1990 Oct;32(2):83–86. doi: 10.1002/jmv.1890320203. [DOI] [PubMed] [Google Scholar]
  30. Weiner A. J., Kuo G., Bradley D. W., Bonino F., Saracco G., Lee C., Rosenblatt J., Choo Q. L., Houghton M. Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet. 1990 Jan 6;335(8680):1–3. doi: 10.1016/0140-6736(90)90134-q. [DOI] [PubMed] [Google Scholar]
  31. Wu J. S., Chen C. H., Chiang Y. H., Lee Y. C., Lee M. H., Ko Y. C., Hu H. T. Hepatitis B virus infection in Taiwan with reference to anti-HBc versus HBsAg and anti-HBs. Taiwan Yi Xue Hui Za Zhi. 1980 Sep;79(9):760–767. [PubMed] [Google Scholar]
  32. Yoshida C. F., Takahashi C., Gaspar A. M., Schatzmayr H. G., Ruzany F. Hepatitis C virus in chronic hemodialysis patients with non-A, non-B hepatitis. Nephron. 1992;60(2):150–153. doi: 10.1159/000186731. [DOI] [PubMed] [Google Scholar]
  33. Zeldis J. B., Depner T. A., Kuramoto I. K., Gish R. G., Holland P. V. The prevalence of hepatitis C virus antibodies among hemodialysis patients. Ann Intern Med. 1990 Jun 15;112(12):958–960. doi: 10.7326/0003-4819-112-12-958. [DOI] [PubMed] [Google Scholar]
  34. van der Poel C. L., Reesink H. W., Lelie P. N., Leentvaar-Kuypers A., Choo Q. L., Kuo G., Houghton M. Anti-hepatitis C antibodies and non-A, non-B post-transfusion hepatitis in The Netherlands. Lancet. 1989 Aug 5;2(8658):297–298. doi: 10.1016/s0140-6736(89)90486-8. [DOI] [PubMed] [Google Scholar]

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