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. 1987 Feb;25(2):350–354. doi: 10.1128/jcm.25.2.350-354.1987

Comparison of TRACK XI fluorometric immunoassay system with other serologic tests for detection of serum antibody to Brucella abortus in cattle.

S M Hall, A W Confer
PMCID: PMC265898  PMID: 3102552

Abstract

The TRACK XI system (TRACK) is a commercially available fluorometric immunoassay system that has been adapted for the detection of antibodies to several antigens in various animal species. Serum antibodies to Brucella abortus were detected by TRACK and compared with those obtained from two primary binding assays (a fluorometric immunoassay [FIAX] and an enzyme-linked immunosorbent assay) and three standard serological tests (complement fixation, Rivanol precipitation, and CARD tests). A total of 298 serum samples were tested by each serological test. Of these serum samples, 134 were negative controls, 43 were from cattle 1 month after vaccination with B. abortus 19, and 121 were from cattle 10 to 12 weeks after a midgestational, intraconjunctival challenge with B. abortus 2308. The results of this study indicated that TRACK is both reproducible and accurate. The results compared favorably with those of other serological methods. TRACK is more rapid than either the enzyme-linked immunosorbent assay or FIAX system. TRACK was the most sensitive (96.3%) test, with a specificity of 100%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berman D. T., Wilson B. L., Moreno E., Angus R. D., Jones L. M. Characterization of Brucella abortus soluble antigen employed in immunoassay. J Clin Microbiol. 1980 Apr;11(4):355–362. doi: 10.1128/jcm.11.4.355-362.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Byrd J. W., Heck F. C., Hidalgo R. J. Evaluation of the enzyme-linked immunosorbent assay for detecting Brucella abortus antibodies. Am J Vet Res. 1979 Jun;40(6):896–898. [PubMed] [Google Scholar]
  3. Cochrane A. L., Holland W. W. Validation of screening procedures. Br Med Bull. 1971 Jan;27(1):3–8. doi: 10.1093/oxfordjournals.bmb.a070810. [DOI] [PubMed] [Google Scholar]
  4. Confer A. W., Hall S. M., Faulkner C. B., Espe B. H., Deyoe B. L., Morton R. J., Smith R. A. Effects of challenge dose on the clinical and immune responses of cattle vaccinated with reduced doses of Brucella abortus strain 19. Vet Microbiol. 1985 Dec;10(6):561–575. doi: 10.1016/0378-1135(85)90065-3. [DOI] [PubMed] [Google Scholar]
  5. Deyoe B. L., Dorsey T. A., Meredith K. B., Garrett L. Effect of reduced dosages of Brucella abortus strain 19 in cattle vaccinated as yearlings. Proc Annu Meet U S Anim Health Assoc. 1979;(83):92–104. [PubMed] [Google Scholar]
  6. Dolan L. A. Latent carriers of brucellosis. Vet Rec. 1980 Mar 15;106(11):241–243. doi: 10.1136/vr.106.11.241. [DOI] [PubMed] [Google Scholar]
  7. Hall S. M., Confer A. W., Tabatabai L. B., Deyoe B. L. Detection of serum antibody to Brucella abortus in cattle by use of a quantitative fluorometric immunoassay. J Clin Microbiol. 1984 Dec;20(6):1023–1027. doi: 10.1128/jcm.20.6.1023-1027.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacobson R. H., Downing D. R., Lynch T. J. Computer-assisted enzyme immunoassays and simplified immunofluorescence assays: applications for the diagnostic laboratory and the veterinarian's office. J Am Vet Med Assoc. 1982 Nov 15;181(10):1166–1168. [PubMed] [Google Scholar]
  9. Morgan W. J. The serological diagnosis of bovine brucellosis. Vet Rec. 1967 May 27;80(21):612–620. doi: 10.1136/vr.80.21.612. [DOI] [PubMed] [Google Scholar]
  10. Phillips W. C., Scott J. A., Blasczcynski G. Statistics for diagnostic procedures. I. How sensitive is "sensitivity"; how specific is "specificity"? AJR Am J Roentgenol. 1983 Jun;140(6):1265–1270. doi: 10.2214/ajr.140.6.1265. [DOI] [PubMed] [Google Scholar]
  11. Stemshorn B. W. Recent progress in the diagnosis of brucellosis. Dev Biol Stand. 1984;56:325–340. [PubMed] [Google Scholar]
  12. Tabatabai L. B., Deyoe B. L. Biochemical and biological properties of soluble protein preparations from Brucella abortus. Dev Biol Stand. 1984;56:199–211. [PubMed] [Google Scholar]
  13. Tabatabai L. B., Deyoe B. L. Specific enzyme-linked immunosorbent assay for detection of bovine antibody to Brucella abortus. J Clin Microbiol. 1984 Aug;20(2):209–213. doi: 10.1128/jcm.20.2.209-213.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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