Abstract
Rapid immunofluorescence (FA) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of FA in a clinical laboratory setting. For respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). The sensitivity of FA for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza A virus, and adenoviruses ranged from 28 to 63%, but specificities for these viruses were uniformly 98 to 100%. The observations form a basis for consideration of selective reduction of routine culture procedures for specimens with initial positive rapid FA results; however, the possibility of dual viral infection in some situations must also be considered.
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- Hendry R. M., Pierik L. T., McIntosh K. Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. J Clin Microbiol. 1986 Feb;23(2):383–384. doi: 10.1128/jcm.23.2.383-384.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hyypiä T. Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes. J Clin Microbiol. 1985 May;21(5):730–733. doi: 10.1128/jcm.21.5.730-733.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kao C. L., McIntosh K., Fernie B., Talis A., Pierik L., Anderson L. Monoclonal antibodies for the rapid diagnosis of respiratory syncytial virus infection by immunofluorescence. Diagn Microbiol Infect Dis. 1984 Jun;2(3):199–206. doi: 10.1016/0732-8893(84)90031-2. [DOI] [PubMed] [Google Scholar]
- McQuillin J., Madeley C. R., Kendal A. P. Monoclonal antibodies for the rapid diagnosis of influenza A and B virus infections by immunofluorescence. Lancet. 1985 Oct 26;2(8461):911–914. doi: 10.1016/s0140-6736(85)90849-9. [DOI] [PubMed] [Google Scholar]
- Minnich L. L., Ray C. G. Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1982 May;15(5):969–970. doi: 10.1128/jcm.15.5.969-970.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vesikari T., Kuusela A. L., Sarkkinen H. K., Halonen P. E. Clinical evaluation of radioimmunoassay of nasopharyngeal secretions and serology for diagnosis of viral infections in children hospitalized for respiratory infections. Pediatr Infect Dis. 1982 Nov-Dec;1(6):391–394. doi: 10.1097/00006454-198211000-00006. [DOI] [PubMed] [Google Scholar]
- Waner J. L., Whitehurst N. J., Jonas S., Wall L., Shalaby H. Isolation of viruses from specimens submitted for direct immunofluorescence test for respiratory syncytial virus. J Pediatr. 1986 Feb;108(2):249–250. doi: 10.1016/s0022-3476(86)80993-3. [DOI] [PubMed] [Google Scholar]
- Wong D. T., Welliver R. C., Riddlesberger K. R., Sun M. S., Ogra P. L. Rapid diagnosis of parainfluenza virus infection in children. J Clin Microbiol. 1982 Jul;16(1):164–167. doi: 10.1128/jcm.16.1.164-167.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]