Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Apr;25(4):641–644. doi: 10.1128/jcm.25.4.641-644.1987

Immunospecific T-lymphocyte stimulation by membrane proteins from Francisella tularensis.

G Sandström, A Tärnvik, H Wolf-Watz
PMCID: PMC266051  PMID: 3494744

Abstract

Membranes from a capsule-deficient mutant of the live-vaccine strain of Francisella tularensis (F. tularensis LVS) were treated with N-lauroyl sarcosinate (Sarkosyl; CIBA-GEIGY Corp., Summit, N.J.). When the Sarkosyl-insoluble fraction was heated in the presence of sodium dodecyl sulfate and mercaptoethanol and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, several polypeptides were distinguished. Four major polypeptides were eluted from the gel, each of which stimulated lymphocytes from tularemia-vaccinated individuals but not from nonvaccinated individuals. The stimulation occurred mainly in T lymphocytes. Radioactive labeling of surface proteins of the capsule-deficient bacteria indicated that at least two of the four polypeptides originated from outer membrane proteins. The results suggest that several membrane proteins of F. tularensis LVS induce a specific T-lymphocyte response.

Full text

PDF
641

Images in this article

642Image
on p.642
643Image
on p.643

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen P. M., Strydom D. J., Unanue E. R. Processing of lysozyme by macrophages: identification of the determinant recognized by two T-cell hybridomas. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2489–2493. doi: 10.1073/pnas.81.8.2489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gabay J. E., Blake M., Niles W. D., Horwitz M. A. Purification of Legionella pneumophila major outer membrane protein and demonstration that it is a porin. J Bacteriol. 1985 Apr;162(1):85–91. doi: 10.1128/jb.162.1.85-91.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hager D. A., Burgess R. R. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem. 1980 Nov 15;109(1):76–86. doi: 10.1016/0003-2697(80)90013-5. [DOI] [PubMed] [Google Scholar]
  4. Hartzman R. J., Bach M. L., Bach F. H., Thurman G. B., Sell K. W. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor. Cell Immunol. 1972 Jun;4(2):182–186. doi: 10.1016/0008-8749(72)90018-4. [DOI] [PubMed] [Google Scholar]
  5. Hood A. M. Virulence factors of Francisella tularensis. J Hyg (Lond) 1977 Aug;79(1):47–60. doi: 10.1017/s0022172400052840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kostiala A. A., McGregor D. D., Logie P. S. Tularaemia in the rat. I. The cellular basis on host resistance to infection. Immunology. 1975 May;28(5):855–869. [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Nakae T., Ishii J., Tokunaga M. Subunit structure of functional porin oligomers that form permeability channels in the other membrane of Escherichia coli. J Biol Chem. 1979 Mar 10;254(5):1457–1461. [PubMed] [Google Scholar]
  10. Newhall W. J., Jones R. B. Disulfide-linked oligomers of the major outer membrane protein of chlamydiae. J Bacteriol. 1983 May;154(2):998–1001. doi: 10.1128/jb.154.2.998-1001.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ottenhoff T. H., Klatser P. R., Ivanyi J., Elferink D. G., de Wit M. Y., de Vries R. R. Mycobacterium leprae-specific protein antigens defined by cloned human helper T cells. Nature. 1986 Jan 2;319(6048):66–68. doi: 10.1038/319066a0. [DOI] [PubMed] [Google Scholar]
  12. Sandström G., Tärnvik A., Wolf-Watz H., Löfgren S. Antigen from Francisella tularensis: nonidentity between determinants participating in cell-mediated and humoral reactions. Infect Immun. 1984 Jul;45(1):101–106. doi: 10.1128/iai.45.1.101-106.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tärnvik A., Holm S. E. Stimulation of subpopulations of human lymphocytes by a vaccine strain of Francisella tularensis. Infect Immun. 1978 Jun;20(3):698–704. doi: 10.1128/iai.20.3.698-704.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tärnvik A., Löfgren M. L., Löfgren S., Sandström G., Wolf-Watz H. Long-lasting cell-mediated immunity induced by a live Francisella tularensis vaccine. J Clin Microbiol. 1985 Oct;22(4):527–530. doi: 10.1128/jcm.22.4.527-530.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tärnvik A., Löfgren S. Stimulation of human lymphocytes by a vaccine strain of Francisella tularensis. Infect Immun. 1975 Nov;12(5):951–957. doi: 10.1128/iai.12.5.951-957.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tärnvik A., Sandström G., Löfgren S. Time of lymphocyte response after onset of tularemia and after tularemia vaccination. J Clin Microbiol. 1979 Dec;10(6):854–860. doi: 10.1128/jcm.10.6.854-860.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van Eden W., de Vries R. R., Stanford J. L., Rook G. A. HLA-DR3 associated genetic control of response to multiple skin tests with new tuberculins. Clin Exp Immunol. 1983 May;52(2):287–292. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES