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. 1987 Apr;25(4):751–753. doi: 10.1128/jcm.25.4.751-753.1987

Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea.

E J Kuijper, J H Oudbier, W N Stuifbergen, A Jansz, H C Zanen
PMCID: PMC266078  PMID: 3033017

Abstract

Two patients in one hospital room acquired pseudomembranous colitis, one shortly after the other. The DNA restriction patterns of isolates from the patients and of four isolates from the environment were indistinguishable from one another and differed from isolates of other patients. Restriction endonuclease digest analysis appears to be a useful method for studying the epidemiology of Clostridium difficile.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett J. G. Antibiotic-associated pseudomembranous colitis. Rev Infect Dis. 1979 May-Jun;1(3):530–539. doi: 10.1093/clinids/1.3.530. [DOI] [PubMed] [Google Scholar]
  2. Bender B. S., Bennett R., Laughon B. E., Greenough W. B., 3rd, Gaydos C., Sears S. D., Forman M. S., Bartlett J. G. Is Clostridium difficile endemic in chronic-care facilities? Lancet. 1986 Jul 5;2(8497):11–13. doi: 10.1016/s0140-6736(86)92559-6. [DOI] [PubMed] [Google Scholar]
  3. Bjorvatn B., Kristiansen B. E. Molecular epidemiology of bacterial infections. Clin Lab Med. 1985 Sep;5(3):437–445. [PubMed] [Google Scholar]
  4. Burdon D. W. Clostridium difficile: the epidemiology and prevention of hospital-acquired infection. Infection. 1982;10(4):203–204. doi: 10.1007/BF01666909. [DOI] [PubMed] [Google Scholar]
  5. Delmee M., Bulliard G., Simon G. Application of a technique for serogrouping Clostridium difficile in an outbreak of antibiotic-associated diarrhoea. J Infect. 1986 Jul;13(1):5–9. doi: 10.1016/s0163-4453(86)92095-5. [DOI] [PubMed] [Google Scholar]
  6. Fekety R., Kim K. H., Brown D., Batts D. H., Cudmore M., Silva J., Jr Epidemiology of antibiotic-associated colitis; isolation of Clostridium difficile from the hospital environment. Am J Med. 1981 Apr;70(4):906–908. doi: 10.1016/0002-9343(81)90553-2. [DOI] [PubMed] [Google Scholar]
  7. Gerding D. N., Olson M. M., Peterson L. R., Teasley D. G., Gebhard R. L., Schwartz M. L., Lee J. T., Jr Clostridium difficile-associated diarrhea and colitis in adults. A prospective case-controlled epidemiologic study. Arch Intern Med. 1986 Jan;146(1):95–100. [PubMed] [Google Scholar]
  8. Greenfield C., Burroughs A., Szawathowski M., Bass N., Noone P., Pounder R. Is pseudomembranous colitis infectious? Lancet. 1981 Feb 14;1(8216):371–372. doi: 10.1016/s0140-6736(81)91683-4. [DOI] [PubMed] [Google Scholar]
  9. Heard S. R., O'Farrell S., Holland D., Crook S., Barnett M. J., Tabaqchali S. The epidemiology of Clostridium difficile with use of a typing scheme: nosocomial acquisition and cross-infection among immunocompromised patients. J Infect Dis. 1986 Jan;153(1):159–162. doi: 10.1093/infdis/153.1.159. [DOI] [PubMed] [Google Scholar]
  10. Kim K. H., Fekety R., Batts D. H., Brown D., Cudmore M., Silva J., Jr, Waters D. Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis. J Infect Dis. 1981 Jan;143(1):42–50. doi: 10.1093/infdis/143.1.42. [DOI] [PubMed] [Google Scholar]
  11. Kuijper E. J., Merrelaar J. J., Stuifbergen W. N. Is pseudomembraneuze colitis besmettelijk? Ned Tijdschr Geneeskd. 1986 Oct 4;130(40):1805–1807. [PubMed] [Google Scholar]
  12. Larson H. E., Price A. B., Honour P., Borriello S. P. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet. 1978 May 20;1(8073):1063–1066. doi: 10.1016/s0140-6736(78)90912-1. [DOI] [PubMed] [Google Scholar]
  13. Loeschke K., Hauck R., Halbritter R., Pfaller P., Ruckdeschel G. Clostridium difficile und Antibiotika-assoziierte Colitis bei Risikopatienten: Zweimonatige epidemiologische Studie auf einer Intensiveinheit. Klin Wochenschr. 1983 Nov 2;61(21):1081–1087. doi: 10.1007/BF01496469. [DOI] [PubMed] [Google Scholar]
  14. Malamou-Ladas H., O'Farrell S., Nash J. Q., Tabaqchali S. Isolation of Clostridium difficile from patients and the environment of hospital wards. J Clin Pathol. 1983 Jan;36(1):88–92. doi: 10.1136/jcp.36.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Poxton I. R., Aronsson B., Möllby R., Nord C. E., Collee J. G. Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis and diarrhoea. J Med Microbiol. 1984 Jun;17(3):317–324. doi: 10.1099/00222615-17-3-317. [DOI] [PubMed] [Google Scholar]
  16. Rietra P. J., Meuwissen S. G., Zanen H. C. Postantibiotische pseudomembraneuze colitis en verwante vormen van diarree na gebruik van antibiotica. II. Microbiologische aspecten. Ned Tijdschr Geneeskd. 1979 Dec 29;123(52):2212–2217. [PubMed] [Google Scholar]
  17. Sell T. L., Schaberg D. R., Fekety F. R. Bacteriophage and bacteriocin typing scheme for Clostridium difficile. J Clin Microbiol. 1983 Jun;17(6):1148–1152. doi: 10.1128/jcm.17.6.1148-1152.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tabaqchali S., Holland D., O'Farrell S., Silman R. Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet. 1984 Apr 28;1(8383):935–938. doi: 10.1016/s0140-6736(84)92392-4. [DOI] [PubMed] [Google Scholar]
  19. Wexler H., Mulligan M. E., Finegold S. M. Polyacrylamide gel electrophoresis patterns produced by Clostridium difficile. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S229–S234. doi: 10.1093/clinids/6.supplement_1.s229. [DOI] [PubMed] [Google Scholar]

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