Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Nov;31(11):2866–2872. doi: 10.1128/jcm.31.11.2866-2872.1993

Detection of antibody to recombinant Norwalk virus antigen in specimens from outbreaks of gastroenteritis.

S S Monroe 1, S E Stine 1, X Jiang 1, M K Estes 1, R I Glass 1
PMCID: PMC266146  PMID: 8263169

Abstract

Norwalk virus and other small round-structured viruses are commonly associated with outbreaks of gastroenteritis. We used a recently described recombinant-expressed Norwalk virus (rNV) capsid protein in enzyme immunoassays to quantitatively measure immunoglobulin G (IgG) and IgA to Norwalk virus in serum pairs from patients involved in outbreaks of gastroenteritis. The outbreaks previously were classified, on the basis of the results of a blocking antibody assay, as Norwalk virus negative, serologically intermediate, or Norwalk virus positive. The rNV IgG assay was more sensitive than the blocking assay for detecting IgG to Norwalk virus in serum from patients in all outbreak classes. There was 79% concordance between seroconversions detected by the blocking antibody assay and those detected by the rNV IgG assay. The rNV IgA assay detected seroconversions to Norwalk virus primarily in patients involved in outbreaks previously classified as Norwalk virus positive.

Full text

PDF
2869

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Leon R., Matsui S. M., Baric R. S., Herrmann J. E., Blacklow N. R., Greenberg H. B., Sobsey M. D. Detection of Norwalk virus in stool specimens by reverse transcriptase-polymerase chain reaction and nonradioactive oligoprobes. J Clin Microbiol. 1992 Dec;30(12):3151–3157. doi: 10.1128/jcm.30.12.3151-3157.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gary G. W., Jr, Kaplan J. E., Stine S. E., Anderson L. J. Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay. J Clin Microbiol. 1985 Aug;22(2):274–278. doi: 10.1128/jcm.22.2.274-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Green K. Y., Lew J. F., Jiang X., Kapikian A. Z., Estes M. K. Comparison of the reactivities of baculovirus-expressed recombinant Norwalk virus capsid antigen with those of the native Norwalk virus antigen in serologic assays and some epidemiologic observations. J Clin Microbiol. 1993 Aug;31(8):2185–2191. doi: 10.1128/jcm.31.8.2185-2191.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greenberg H. B., Wyatt R. G., Valdesuso J., Kalica A. R., London W. T., Chanock R. M., Kapikian A. Z. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. doi: 10.1002/jmv.1890020204. [DOI] [PubMed] [Google Scholar]
  5. Hayashi Y., Ando T., Utagawa E., Sekine S., Okada S., Yabuuchi K., Miki T., Ohashi M. Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo. J Clin Microbiol. 1989 Aug;27(8):1728–1733. doi: 10.1128/jcm.27.8.1728-1733.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jiang X., Wang J., Graham D. Y., Estes M. K. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2529–2534. doi: 10.1128/jcm.30.10.2529-2534.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jiang X., Wang M., Graham D. Y., Estes M. K. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. doi: 10.1128/jvi.66.11.6527-6532.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jiang X., Wang M., Wang K., Estes M. K. Sequence and genomic organization of Norwalk virus. Virology. 1993 Jul;195(1):51–61. doi: 10.1006/viro.1993.1345. [DOI] [PubMed] [Google Scholar]
  9. Kaplan J. E., Gary G. W., Baron R. C., Singh N., Schonberger L. B., Feldman R., Greenberg H. B. Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis. Ann Intern Med. 1982 Jun;96(6 Pt 1):756–761. doi: 10.7326/0003-4819-96-6-756. [DOI] [PubMed] [Google Scholar]
  10. Keswick B. H., Satterwhite T. K., Johnson P. C., DuPont H. L., Secor S. L., Bitsura J. A., Gary G. W., Hoff J. C. Inactivation of Norwalk virus in drinking water by chlorine. Appl Environ Microbiol. 1985 Aug;50(2):261–264. doi: 10.1128/aem.50.2.261-264.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lambden P. R., Caul E. O., Ashley C. R., Clarke I. N. Sequence and genome organization of a human small round-structured (Norwalk-like) virus. Science. 1993 Jan 22;259(5094):516–519. doi: 10.1126/science.8380940. [DOI] [PubMed] [Google Scholar]
  12. Lewis D. C. Three serotypes of Norwalk-like virus demonstrated by solid-phase immune electron microscopy. J Med Virol. 1990 Jan;30(1):77–81. doi: 10.1002/jmv.1890300117. [DOI] [PubMed] [Google Scholar]
  13. Madore H. P., Treanor J. J., Buja R., Dolin R. Antigenic relatedness among the Norwalk-like agents by serum antibody rises. J Med Virol. 1990 Oct;32(2):96–101. doi: 10.1002/jmv.1890320206. [DOI] [PubMed] [Google Scholar]
  14. Madore H. P., Treanor J. J., Pray K. A., Dolin R. Enzyme-linked immunosorbent assays for Snow Mountain and Norwalk agents of viral gastroenteritis. J Clin Microbiol. 1986 Sep;24(3):456–459. doi: 10.1128/jcm.24.3.456-459.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Matsui S. M., Kim J. P., Greenberg H. B., Su W., Sun Q., Johnson P. C., DuPont H. L., Oshiro L. S., Reyes G. R. The isolation and characterization of a Norwalk virus-specific cDNA. J Clin Invest. 1991 Apr;87(4):1456–1461. doi: 10.1172/JCI115152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okada S., Sekine S., Ando T., Hayashi Y., Murao M., Yabuuchi K., Miki T., Ohashi M. Antigenic characterization of small, round-structured viruses by immune electron microscopy. J Clin Microbiol. 1990 Jun;28(6):1244–1248. doi: 10.1128/jcm.28.6.1244-1248.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Plikaytis B. D., Turner S. H., Gheesling L. L., Carlone G. M. Comparisons of standard curve-fitting methods to quantitate Neisseria meningitidis group A polysaccharide antibody levels by enzyme-linked immunosorbent assay. J Clin Microbiol. 1991 Jul;29(7):1439–1446. doi: 10.1128/jcm.29.7.1439-1446.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Treanor J. J., Jiang X., Madore H. P., Estes M. K. Subclass-specific serum antibody responses to recombinant Norwalk virus capsid antigen (rNV) in adults infected with Norwalk, Snow Mountain, or Hawaii virus. J Clin Microbiol. 1993 Jun;31(6):1630–1634. doi: 10.1128/jcm.31.6.1630-1634.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Treanor J. J., Madore H. P., Dolin R. Development of an enzyme immunoassay for the Hawaii agent of viral gastroenteritis. J Virol Methods. 1988 Dec;22(2-3):207–214. doi: 10.1016/0166-0934(88)90103-6. [DOI] [PubMed] [Google Scholar]
  20. Xi J. N., Graham D. Y., Wang K. N., Estes M. K. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. doi: 10.1126/science.2177224. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES