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. 1993 Nov;31(11):2996–3000. doi: 10.1128/jcm.31.11.2996-3000.1993

Rapid detection of Streptococcus pyogenes in pediatric patient specimens by DNA probe.

L L Steed 1, E K Korgenski 1, J A Daly 1
PMCID: PMC266185  PMID: 8263185

Abstract

A chemiluminescent DNA probe test (Group A Streptococcus Direct Test; Gen-Probe, Inc., San Diego, Calif.) for rapid, direct detection of cRNA of Streptococcus pyogenes in throat swabs was compared with conventional culture and identification techniques. Throat swabs from 277 patients suspected of having streptococcal pharyngitis were examined. By DNA probe alone, 10 specimens were positive, 51 were positive by both assays, and 8 were positive by culture alone. Thus, DNA probe sensitivity, specificity, and positive and negative predictive values were 86, 95, 84, and 96%, respectively. Including an indeterminate category, sensitivity, specificity, and positive and negative predictive values were 89, 96, 86, and 97%, respectively. After discrepancy testing, these values for the raw data improved to 90, 98, 93, and 97%, respectively. None of the 24 specimens that grew non-S. pyogenes beta-hemolytic streptococci in culture were positive by the DNA probe. Because mucoid S. pyogenes strains are more virulent than nonmucoid strains, 24 isolates were retrospectively tested with the DNA probe to ensure that both types would be detected equally well. Isolates were examined in pure cultures as well as mixed with representative normal oral flora. There was no statistical difference in detection of any of the four groups. Group A Streptococcus Direct Test is a rapid, sensitive, and specific test for S. pyogenes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bisno A. L. Group A streptococcal infections and acute rheumatic fever. N Engl J Med. 1991 Sep 12;325(11):783–793. doi: 10.1056/NEJM199109123251106. [DOI] [PubMed] [Google Scholar]
  2. Daly J. A., Clifton N. L., Seskin K. C., Gooch W. M., 3rd Use of rapid, nonradioactive DNA probes in culture confirmation tests to detect Streptococcus agalactiae, Haemophilus influenzae, and Enterococcus spp. from pediatric patients with significant infections. J Clin Microbiol. 1991 Jan;29(1):80–82. doi: 10.1128/jcm.29.1.80-82.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Danjani A. S., Bisno A. L., Chung K. J., Durack D. T., Gerber M. A., Kaplan E. L., Millard H. D., Randolph M. F., Shulman S. T., Watanakunakorn C. Prevention of rheumatic fever. A statement for health professionals by the Committee on Rheumatic Fever, Endocarditis, and Kawasaki Disease of the Council on Cardiovascular Disease in the Young, the American Heart Association. Circulation. 1988 Oct;78(4):1082–1086. doi: 10.1161/01.cir.78.4.1082. [DOI] [PubMed] [Google Scholar]
  4. Gerber M. A. Comparison of throat cultures and rapid strep tests for diagnosis of streptococcal pharyngitis. Pediatr Infect Dis J. 1989 Nov;8(11):820–824. doi: 10.1097/00006454-198911000-00032. [DOI] [PubMed] [Google Scholar]
  5. Gerber M. A., Spadaccini L. J., Wright L. L., Deutsch L. Latex agglutination tests for rapid identification of group A streptococci directly from throat swabs. J Pediatr. 1984 Nov;105(5):702–705. doi: 10.1016/s0022-3476(84)80286-3. [DOI] [PubMed] [Google Scholar]
  6. Goto M., Oka S., Okuzumi K., Kimura S., Shimada K. Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture. J Clin Microbiol. 1991 Nov;29(11):2473–2476. doi: 10.1128/jcm.29.11.2473-2476.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoffmann S. Detection of group A streptococcal antigen from throat swabs with five diagnostic kits in general practice. Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):209–215. doi: 10.1016/0732-8893(90)90061-y. [DOI] [PubMed] [Google Scholar]
  8. Kellogg J. A. Suitability of throat culture procedures for detection of group A streptococci and as reference standards for evaluation of streptococcal antigen detection kits. J Clin Microbiol. 1990 Feb;28(2):165–169. doi: 10.1128/jcm.28.2.165-169.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lees M. I., Newnan D. M., Garland S. M. Comparison of a DNA probe assay with culture for the detection of Chlamydia trachomatis. J Med Microbiol. 1991 Sep;35(3):159–161. doi: 10.1099/00222615-35-3-159. [DOI] [PubMed] [Google Scholar]
  10. Mackenzie A. M., Li M. M., Chan F. T. Evaluation of a kit for rapid detection of group A streptococci in a pediatric emergency department. CMAJ. 1988 May 15;138(10):917–919. [PMC free article] [PubMed] [Google Scholar]
  11. Panke E. S., Yang L. I., Leist P. A., Magevney P., Fry R. J., Lee R. F. Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens. J Clin Microbiol. 1991 May;29(5):883–888. doi: 10.1128/jcm.29.5.883-888.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roddey O. F., Clegg H. W., Clardy L. T., Martin E. S., Swetenburg R. L. Comparison of a latex agglutination test and four culture methods for identification of group A streptococci in a pediatric office laboratory. J Pediatr. 1986 Mar;108(3):347–351. doi: 10.1016/s0022-3476(86)80871-x. [DOI] [PubMed] [Google Scholar]
  13. Roddey O. F., Jr Quantitation of group A beta-hemolytic streptococci in throat culture. J Clin Microbiol. 1991 Jun;29(6):1279–1280. doi: 10.1128/jcm.29.6.1279-1280.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwabe L. D., Gobbo A. F., Gottschall R. L., Randall E. L. Comparison of TestPack Plus Strep A with selective and nonselective culture media for detection of group-A streptococci. Diagn Microbiol Infect Dis. 1991 Sep-Oct;14(5):367–372. doi: 10.1016/0732-8893(91)90062-k. [DOI] [PubMed] [Google Scholar]
  15. Shulman S. T. Streptococcal pharyngitis: clinical and epidemiologic factors. Pediatr Infect Dis J. 1989 Nov;8(11):816–819. [PubMed] [Google Scholar]
  16. Stevens D. L., Tanner M. H., Winship J., Swarts R., Ries K. M., Schlievert P. M., Kaplan E. Severe group A streptococcal infections associated with a toxic shock-like syndrome and scarlet fever toxin A. N Engl J Med. 1989 Jul 6;321(1):1–7. doi: 10.1056/NEJM198907063210101. [DOI] [PubMed] [Google Scholar]
  17. Veasy L. G., Wiedmeier S. E., Orsmond G. S., Ruttenberg H. D., Boucek M. M., Roth S. J., Tait V. F., Thompson J. A., Daly J. A., Kaplan E. L. Resurgence of acute rheumatic fever in the intermountain area of the United States. N Engl J Med. 1987 Feb 19;316(8):421–427. doi: 10.1056/NEJM198702193160801. [DOI] [PubMed] [Google Scholar]

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